LOCUS       Exported     3268 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3268
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             2010..3254
                     /translation="MFAYTKNKKKLAKHKPKKNRSFAIVIPAHNESMVVGKLIDSIKAQKYDGVIDIYLVADNCTDNRKTYNVGIEKGITVLERFHSTLKGGNFAIRHGWRYIRDNNLLDKYDCFCSFDADNLLDENWVYEVNKTFDFYNDIEVVTTYRNSKNYADNWISSACSIQFLKESDIINKGRATLNHSSYVNGTGFCITRKIFEQTNWWDFNSLSHDIEFTQWLMLNKIKTGYAPNACFYDEQPIDFKNSWKQRMRWCVGFKQVWKIYRSEMIKNMFTFKINKIKLWVNFTMIFPAVITLLINLLFWLITFGLIISNYIVNYLNSSLVIIEQANNYLRDLIIYCTTTPIVIFGIIFINYLLWGFIIVARNKKSIQATSWQKFKSIFTYPFFMLTYIPISFLALFKSTYSTTPVARKAQLVQN"
                     /label="MMSYN1_0113"
                     /note="glycosyltransferase, group 2 family protein"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGTTTGCTTA TACCAAAAAC AAGAAAAAGT
     2041 TGGCGAAACA TAAGCCAAAG AAGAACCGCT CTTTTGCCAT CGTTATTCCG GCGCACAATG
     2101 AATCGATGGT AGTGGGTAAG TTGATTGACT CTATTAAGGC GCAGAAGTAT GACGGCGTCA
     2161 TCGATATTTA CTTAGTCGCT GATAATTGTA CAGATAACCG CAAGACGTAC AACGTGGGAA
     2221 TTGAAAAAGG CATCACTGTT TTAGAACGTT TTCACAGCAC CTTAAAGGGG GGTAACTTCG
     2281 CAATTCGTCA CGGCTGGCGT TACATTCGTG ACAATAACTT GTTGGATAAG TATGATTGTT
     2341 TTTGCTCCTT TGATGCCGAT AACCTTCTTG ACGAAAATTG GGTGTACGAA GTCAACAAAA
     2401 CATTCGACTT CTATAACGAT ATCGAGGTAG TAACTACATA CCGTAACAGC AAGAACTACG
     2461 CCGATAATTG GATCTCAAGC GCATGTAGTA TCCAGTTTTT AAAAGAATCA GATATCATTA
     2521 ATAAGGGGCG TGCTACCTTG AACCATTCCT CTTACGTGAA CGGAACGGGC TTTTGTATCA
     2581 CCCGTAAGAT CTTTGAGCAA ACTAATTGGT GGGATTTCAA CTCCCTTTCT CACGACATCG
     2641 AGTTCACTCA ATGGCTGATG TTGAATAAGA TTAAAACCGG TTATGCCCCA AATGCTTGCT
     2701 TCTACGACGA GCAGCCCATC GATTTCAAGA ATTCATGGAA ACAACGCATG CGCTGGTGTG
     2761 TTGGGTTCAA ACAGGTCTGG AAAATCTATC GCTCCGAGAT GATTAAGAAT ATGTTTACCT
     2821 TTAAAATTAA CAAAATCAAG CTGTGGGTAA ATTTTACTAT GATCTTCCCG GCGGTTATCA
     2881 CCTTACTGAT TAACCTTTTG TTCTGGCTGA TTACATTCGG CTTAATTATC TCTAATTACA
     2941 TCGTCAACTA TCTTAACAGC TCTCTTGTTA TCATCGAGCA AGCTAACAAT TATCTTCGCG
     3001 ATCTTATTAT TTATTGTACC ACTACTCCTA TTGTTATTTT CGGGATTATT TTCATTAATT
     3061 ACCTTCTTTG GGGTTTCATT ATTGTGGCCC GTAATAAGAA ATCGATCCAG GCTACATCGT
     3121 GGCAGAAATT CAAATCAATT TTCACATACC CCTTCTTCAT GTTGACGTAT ATCCCGATCA
     3181 GTTTCTTGGC GTTGTTTAAA TCGACTTACA GCACGACCCC CGTAGCGCGT AAAGCGCAAT
     3241 TAGTTCAAAA CTGAAGAGCT TAGAGACC
//