LOCUS       Exported     2930 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2930
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      943..990
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2818..2834
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2835..2866
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /note="galU: UTP--glucose-1-phosphate uridylyltransferase"
                     /translation="MTIRKAVIPCAGFGTRFLPFTKSQAKEMLPIIDTPTIEYIVKEAIDSGIKEILIVLNDKKSEIMNYFSRNIELERFLYNKDKINELEQIKTKYDADIHYIMQDEPLGLGHAISLCKGFINNESFAVLLGDDLFKCQTPAIKQLMDLYEEKHSTILGTILIDKKDCKKYGICKTESSNDNVYKVCSVVEKPDEQNAPSNVAIAGRYILTPEIFNYLDLQLKGETGEIELTDSILRTIKDVDCYAKVIDGKRYDIGNKLGYLEAFVDFASNRDDTKNEFIKIVKKLNEEETL"
                     /label="MMSYN1_0115"
     primer_bind     complement(995..1011)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(1078..1666)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(1837..2697)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(2698..2802)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /translation="MTIRKAVIPCAGFGTRFLPFTKSQAKEMLPIIDTPTIEYIVKEAIDSGIKEILIVLNDKKSEIMNYFSRNIELERFLYNKDKINELEQIKTKYDADIHYIMQDEPLGLGHAISLCKGFINNESFAVLLGDDLFKCQTPAIKQLMDLYEEKHSTILGTILIDKKDCKKYGICKTESSNDNVYKVCSVVEKPDEQNAPSNVAIAGRYILTPEIFNYLDLQLKGETGEIELTDSILRTIKDVDCYAKVIDGKRYDIGNKLGYLEAFVDFASNRDDTKNEFIKIVKKLNEEETL"
                     /label="MMSYN1_0115"
                     /note="galU: UTP--glucose-1-phosphate uridylyltransferase"
ORIGIN
        1 ATGACTATTC GCAAAGCAGT AATTCCTTGT GCAGGGTTTG GGACCCGTTT TTTACCTTTT
       61 ACAAAGAGTC AGGCGAAAGA GATGTTGCCT ATTATTGACA CCCCAACAAT CGAGTACATT
      121 GTGAAAGAGG CAATTGACTC GGGGATTAAA GAGATTCTGA TTGTCCTGAA TGACAAGAAA
      181 TCTGAGATCA TGAACTATTT TAGCCGCAAC ATTGAATTGG AGCGCTTCCT GTATAACAAG
      241 GATAAAATCA ATGAATTAGA ACAAATTAAG ACAAAGTACG ACGCAGATAT TCACTATATT
      301 ATGCAGGACG AGCCCTTGGG TCTGGGCCAT GCGATCTCCT TATGTAAGGG CTTTATCAAT
      361 AATGAATCTT TCGCAGTGTT ACTGGGCGAC GATTTATTCA AGTGCCAAAC TCCAGCAATC
      421 AAGCAGTTGA TGGATCTTTA CGAAGAGAAG CATTCGACCA TTTTAGGGAC GATCTTAATC
      481 GATAAAAAGG ATTGCAAGAA GTATGGTATT TGCAAAACTG AGTCCTCTAA TGACAACGTC
      541 TATAAAGTCT GCTCAGTAGT GGAGAAACCG GACGAGCAGA ATGCACCCAG CAACGTGGCA
      601 ATTGCAGGCC GTTACATTTT GACGCCTGAG ATTTTCAACT ACTTGGATTT GCAACTTAAA
      661 GGAGAAACCG GCGAAATTGA GCTTACAGAT AGTATCTTGC GTACTATCAA GGATGTTGAT
      721 TGCTACGCCA AAGTAATCGA TGGAAAACGT TATGATATTG GGAATAAGTT GGGGTATTTA
      781 GAAGCATTTG TGGATTTCGC GAGTAACCGT GATGATACCA AAAATGAGTT CATCAAAATC
      841 GTAAAAAAGT TAAATGAAGA AGAGACACTG TGAAGAGCTT AGAGACCGGT ACTAGTTCCT
      901 GCAGGGCTCG AGGTGTCAAT CGTCGGAGCC GCTGAGCAAT AACTAGCATA ACCCCTTGGG
      961 GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CATGGTCATA GCTGTTTCCT GAGAGCTTGG
     1021 CAGGTGATGA CACACATTAA CAAATTTCGT GAGGAGTCTC CAGAAGAATG CCATTAATTT
     1081 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG
     1141 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC
     1201 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT
     1261 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA
     1321 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA
     1381 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA
     1441 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA
     1501 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT
     1561 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT
     1621 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT
     1681 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT
     1741 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC
     1801 AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC
     1861 ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA
     1921 GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA
     1981 ACCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG
     2041 CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC
     2101 TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT
     2161 CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG
     2221 GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT
     2281 CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA
     2341 TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA
     2401 GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA
     2461 TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG
     2521 GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC
     2581 ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG
     2641 AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT
     2701 CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT
     2761 ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACCTGCA CCAGTCAGTA
     2821 AAACGACGGC CAGTAGTCAA AAGCCTCCGA CCGGAGGCTT TTGACTTGGT CAGGTGGAGT
     2881 GAATTCGCGG CCGCTTCTAG AGGCTCTTCG TAATTACGAA GAGGTCTCTA
//