LOCUS       Exported     2498 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2498
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      511..558
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2386..2402
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      2403..2434
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0116"
                     /note=""
                     /translation="MQNKSGLILLKEVFINNYSNKIDFLKTVFSDKQINELESITNIKELLTNLKELLDNQILIHQNKIKEYQLELKKTNKKILNKLWLWWLLPIIGMFIFFIIYNTRLQNPYYANQLVDIKVKITDLDIKNIYIDKLLEEINSSVKLKF"
     primer_bind     complement(563..579)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(646..1234)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1405..2265)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2266..2370)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0116"
                     /note=""
                     /translation="MQNKSGLILLKEVFINNYSNKIDFLKTVFSDKQINELESITNIKELLTNLKELLDNQILIHQNKIKEYQLELKKTNKKILNKLWLWWLLPIIGMFIFFIIYNTRLQNPYYANQLVDIKVKITDLDIKNIYIDKLLEEINSSVKLKF"
ORIGIN
        1 ATGCAAAACA AGTCCGGCTT GATTCTGTTA AAGGAAGTAT TCATTAATAA CTATAGCAAC
       61 AAAATCGACT TCCTTAAAAC AGTGTTCTCT GACAAGCAAA TCAATGAATT GGAGTCGATT
      121 ACAAACATCA AGGAATTGTT AACCAATCTG AAAGAATTGT TAGACAACCA GATCCTTATC
      181 CATCAGAATA AAATCAAAGA ATATCAACTG GAACTGAAAA AGACCAATAA GAAAATCCTG
      241 AATAAGTTGT GGTTATGGTG GTTACTGCCT ATCATTGGGA TGTTTATCTT TTTCATCATT
      301 TATAACACCC GTCTGCAGAA TCCGTATTAC GCGAACCAGT TGGTAGATAT CAAGGTAAAG
      361 ATTACCGATT TGGATATTAA GAACATCTAC ATCGACAAAC TGCTTGAGGA GATTAACTCA
      421 TCTGTAAAAT TGAAATTTTG AAGAGCTTAG AGACCGGTAC TAGTTCCTGC AGGGCTCGAG
      481 GTGTCAATCG TCGGAGCCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG
      541 GTCTTGAGGG GTTTTTTGCA TGGTCATAGC TGTTTCCTGA GAGCTTGGCA GGTGATGACA
      601 CACATTAACA AATTTCGTGA GGAGTCTCCA GAAGAATGCC ATTAATTTCC ATAGGCTCCG
      661 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG
      721 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC
      781 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA
      841 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT
      901 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC
      961 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG
     1021 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC
     1081 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT
     1141 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA
     1201 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG
     1261 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA
     1321 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT
     1381 ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC
     1441 GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT
     1501 ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGAAC CACGCTCACC
     1561 GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC
     1621 TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG
     1681 TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG
     1741 CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG
     1801 ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG
     1861 TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT
     1921 CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA
     1981 ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC
     2041 ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC
     2101 AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC
     2161 TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC
     2221 CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA
     2281 ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT
     2341 TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACCTGCACC AGTCAGTAAA ACGACGGCCA
     2401 GTAGTCAAAA GCCTCCGACC GGAGGCTTTT GACTTGGTCA GGTGGAGTGA ATTCGCGGCC
     2461 GCTTCTAGAG GCTCTTCGTA ATTACGAAGA GGTCTCTA
//