LOCUS Exported 2819 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..2819 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 832..879 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 2707..2723 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 2724..2755 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 1..0 /translation="MHYLGIIIASVIGYFLGSISWSIIIVKKVGNIDIRTVGSGNPGATNTVRALGKRWGLVVAFLDALKVVFTAISAILLSMIPNDLFSQTSYFIPCIFALIGHCYPIYYKFKGGKAVSCFLGLLFVVNVLYLIIFLIIWFISVAISRKVSVASMFSALIILLIMWIPYLSGVSYFIWEWNGLEKFSFAWKNYILFSLLNSFHYWLSNTWASGILEGNIIILIGGLILAWRHSQNIKRIKNKTEPDTFPKKVKPVR" /label="MMSYN1_0117" /note="Catalyzes the transfer of an acyl group from acyl-phosphate (acyl-PO(4)) to glycerol-3-phosphate (G3P) to form lysophosphatidic acid (LPA). This enzyme utilizes acyl-phosphate as fatty acyl donor, but not acyl-CoA or acyl-ACP." primer_bind complement(884..900) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(967..1555) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1726..2586) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(2587..2691) /label="bla promoter" /note="ampicillin resistance gene promoter" CDS complement(1..0) /note="Catalyzes the transfer of an acyl group from acyl-phosphate (acyl-PO(4)) to glycerol-3-phosphate (G3P) to form lysophosphatidic acid (LPA). This enzyme utilizes acyl-phosphate as fatty acyl donor, but not acyl-CoA or acyl-ACP." /translation="MHYLGIIIASVIGYFLGSISWSIIIVKKVGNIDIRTVGSGNPGATNTVRALGKRWGLVVAFLDALKVVFTAISAILLSMIPNDLFSQTSYFIPCIFALIGHCYPIYYKFKGGKAVSCFLGLLFVVNVLYLIIFLIIWFISVAISRKVSVASMFSALIILLIMWIPYLSGVSYFIWEWNGLEKFSFAWKNYILFSLLNSFHYWLSNTWASGILEGNIIILIGGLILAWRHSQNIKRIKNKTEPDTFPKKVKPVR" /label="MMSYN1_0117" ORIGIN 1 ATGCATTACT TGGGGATTAT CATTGCTAGC GTCATCGGCT ACTTCCTTGG ATCGATTTCA 61 TGGAGTATTA TCATCGTTAA AAAAGTTGGT AACATTGATA TTCGCACGGT TGGCTCTGGT 121 AACCCCGGCG CAACGAACAC AGTTCGCGCC TTGGGTAAGC GCTGGGGTCT TGTGGTAGCA 181 TTCTTAGACG CACTGAAAGT TGTGTTTACC GCAATCAGTG CAATTCTTTT AAGTATGATT 241 CCAAACGACC TGTTCAGTCA AACCAGCTAT TTCATTCCGT GCATCTTCGC GTTAATCGGG 301 CACTGTTACC CTATCTACTA CAAGTTCAAA GGGGGCAAGG CCGTGTCTTG CTTTCTTGGT 361 TTGTTATTTG TTGTTAACGT CTTATATTTG ATCATTTTCT TGATCATCTG GTTCATCTCT 421 GTCGCTATTT CCCGCAAAGT CTCTGTGGCA TCAATGTTTT CGGCCTTGAT CATTCTTTTA 481 ATTATGTGGA TTCCTTACTT AAGCGGGGTG TCATACTTCA TTTGGGAGTG GAACGGTCTT 541 GAGAAGTTTT CATTTGCGTG GAAGAACTAC ATTCTTTTCA GCTTACTTAA CTCTTTCCAT 601 TATTGGCTTA GCAACACTTG GGCCTCAGGT ATTTTAGAAG GGAATATCAT TATTCTTATT 661 GGTGGACTTA TCCTTGCATG GCGTCACTCC CAAAACATTA AACGTATTAA GAACAAAACT 721 GAACCGGATA CATTCCCCAA GAAGGTTAAG CCTGTACGCT GAAGAGCTTA GAGACCGGTA 781 CTAGTTCCTG CAGGGCTCGA GGTGTCAATC GTCGGAGCCG CTGAGCAATA ACTAGCATAA 841 CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC ATGGTCATAG CTGTTTCCTG 901 AGAGCTTGGC AGGTGATGAC ACACATTAAC AAATTTCGTG AGGAGTCTCC AGAAGAATGC 961 CATTAATTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 1021 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 1081 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 1141 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 1201 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 1261 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 1321 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 1381 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 1441 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 1501 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 1561 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 1621 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 1681 TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT 1741 CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC 1801 CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT 1861 ACCGCGAGAA CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG 1921 GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG 1981 CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC 2041 TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA 2101 ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG 2161 TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC 2221 ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA 2281 CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC 2341 AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG 2401 TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC 2461 CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC 2521 AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT 2581 ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG 2641 CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACCTGCAC 2701 CAGTCAGTAA AACGACGGCC AGTAGTCAAA AGCCTCCGAC CGGAGGCTTT TGACTTGGTC 2761 AGGTGGAGTG AATTCGCGGC CGCTTCTAGA GGCTCTTCGT AATTACGAAG AGGTCTCTA //