LOCUS       Exported     2819 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2819
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      832..879
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2707..2723
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2724..2755
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /translation="MHYLGIIIASVIGYFLGSISWSIIIVKKVGNIDIRTVGSGNPGATNTVRALGKRWGLVVAFLDALKVVFTAISAILLSMIPNDLFSQTSYFIPCIFALIGHCYPIYYKFKGGKAVSCFLGLLFVVNVLYLIIFLIIWFISVAISRKVSVASMFSALIILLIMWIPYLSGVSYFIWEWNGLEKFSFAWKNYILFSLLNSFHYWLSNTWASGILEGNIIILIGGLILAWRHSQNIKRIKNKTEPDTFPKKVKPVR"
                     /label="MMSYN1_0117"
                     /note="Catalyzes the transfer of an acyl group from acyl-phosphate (acyl-PO(4)) to glycerol-3-phosphate (G3P) to form lysophosphatidic acid (LPA). This enzyme utilizes acyl-phosphate as fatty acyl donor, but not acyl-CoA or acyl-ACP."
     primer_bind     complement(884..900)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(967..1555)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1726..2586)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2587..2691)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /note="Catalyzes the transfer of an acyl group from acyl-phosphate (acyl-PO(4)) to glycerol-3-phosphate (G3P) to form lysophosphatidic acid (LPA). This enzyme utilizes acyl-phosphate as fatty acyl donor, but not acyl-CoA or acyl-ACP."
                     /translation="MHYLGIIIASVIGYFLGSISWSIIIVKKVGNIDIRTVGSGNPGATNTVRALGKRWGLVVAFLDALKVVFTAISAILLSMIPNDLFSQTSYFIPCIFALIGHCYPIYYKFKGGKAVSCFLGLLFVVNVLYLIIFLIIWFISVAISRKVSVASMFSALIILLIMWIPYLSGVSYFIWEWNGLEKFSFAWKNYILFSLLNSFHYWLSNTWASGILEGNIIILIGGLILAWRHSQNIKRIKNKTEPDTFPKKVKPVR"
                     /label="MMSYN1_0117"
ORIGIN
        1 ATGCATTACT TGGGGATTAT CATTGCTAGC GTCATCGGCT ACTTCCTTGG ATCGATTTCA
       61 TGGAGTATTA TCATCGTTAA AAAAGTTGGT AACATTGATA TTCGCACGGT TGGCTCTGGT
      121 AACCCCGGCG CAACGAACAC AGTTCGCGCC TTGGGTAAGC GCTGGGGTCT TGTGGTAGCA
      181 TTCTTAGACG CACTGAAAGT TGTGTTTACC GCAATCAGTG CAATTCTTTT AAGTATGATT
      241 CCAAACGACC TGTTCAGTCA AACCAGCTAT TTCATTCCGT GCATCTTCGC GTTAATCGGG
      301 CACTGTTACC CTATCTACTA CAAGTTCAAA GGGGGCAAGG CCGTGTCTTG CTTTCTTGGT
      361 TTGTTATTTG TTGTTAACGT CTTATATTTG ATCATTTTCT TGATCATCTG GTTCATCTCT
      421 GTCGCTATTT CCCGCAAAGT CTCTGTGGCA TCAATGTTTT CGGCCTTGAT CATTCTTTTA
      481 ATTATGTGGA TTCCTTACTT AAGCGGGGTG TCATACTTCA TTTGGGAGTG GAACGGTCTT
      541 GAGAAGTTTT CATTTGCGTG GAAGAACTAC ATTCTTTTCA GCTTACTTAA CTCTTTCCAT
      601 TATTGGCTTA GCAACACTTG GGCCTCAGGT ATTTTAGAAG GGAATATCAT TATTCTTATT
      661 GGTGGACTTA TCCTTGCATG GCGTCACTCC CAAAACATTA AACGTATTAA GAACAAAACT
      721 GAACCGGATA CATTCCCCAA GAAGGTTAAG CCTGTACGCT GAAGAGCTTA GAGACCGGTA
      781 CTAGTTCCTG CAGGGCTCGA GGTGTCAATC GTCGGAGCCG CTGAGCAATA ACTAGCATAA
      841 CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC ATGGTCATAG CTGTTTCCTG
      901 AGAGCTTGGC AGGTGATGAC ACACATTAAC AAATTTCGTG AGGAGTCTCC AGAAGAATGC
      961 CATTAATTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA
     1021 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT
     1081 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC
     1141 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT
     1201 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC
     1261 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC
     1321 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG
     1381 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC
     1441 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG
     1501 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA
     1561 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT
     1621 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG
     1681 TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT
     1741 CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC
     1801 CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT
     1861 ACCGCGAGAA CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG
     1921 GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG
     1981 CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC
     2041 TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA
     2101 ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG
     2161 TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC
     2221 ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA
     2281 CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC
     2341 AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG
     2401 TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC
     2461 CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC
     2521 AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT
     2581 ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG
     2641 CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACCTGCAC
     2701 CAGTCAGTAA AACGACGGCC AGTAGTCAAA AGCCTCCGAC CGGAGGCTTT TGACTTGGTC
     2761 AGGTGGAGTG AATTCGCGGC CGCTTCTAGA GGCTCTTCGT AATTACGAAG AGGTCTCTA
//