LOCUS       Exported     3509 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3509
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1522..1569
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3397..3413
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3414..3445
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0126"
                     /note="Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)."
                     /translation="MSFRLRYAPSPTGFLHIGNTRTALMNYLFAKHYNGSFIVRIEDTDLTRNVDGAIESQFENLNWLGIFPDESIFNVGDQKYGKYMQSQKFDRYKQLAEQLVDQNKAYRCFCTSEELEKDYEEQTSKGIIATKYSQKCLFLTQDQIQKNLENKKEYSIRFKVPENKTWTINDIVRGDVSFDSKDLGDFVILKSNGVATYNFAVVIDDYDMQITHVLRGEEHISNTPRQMMIYDAFNWNYPKFGHLTLIVDNTGKKLSKRSGNALFFIEQYKKQGYLSQAIFNYIALLGWSPPGEQEILSQNELIKIFDEKRFSKSPSTFDMVKMKWINSVYMKKLDDDKYLEFVKSFINTNKFDITSKSEAWLNHLLLLYKKELEYAEQINDHLDLFFNKNTLDNNTIDVLNNLTNYKNVVEIFKNQINDLKDWTIENIKQIIKNTSTLANVKGKDLFMPIRIFATKSEHGPSLADVIYLLGKTTVLNNINSLER"
     primer_bind     complement(1574..1590)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1657..2245)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2416..3276)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(3277..3381)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0126"
                     /note="Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)."
                     /translation="MSFRLRYAPSPTGFLHIGNTRTALMNYLFAKHYNGSFIVRIEDTDLTRNVDGAIESQFENLNWLGIFPDESIFNVGDQKYGKYMQSQKFDRYKQLAEQLVDQNKAYRCFCTSEELEKDYEEQTSKGIIATKYSQKCLFLTQDQIQKNLENKKEYSIRFKVPENKTWTINDIVRGDVSFDSKDLGDFVILKSNGVATYNFAVVIDDYDMQITHVLRGEEHISNTPRQMMIYDAFNWNYPKFGHLTLIVDNTGKKLSKRSGNALFFIEQYKKQGYLSQAIFNYIALLGWSPPGEQEILSQNELIKIFDEKRFSKSPSTFDMVKMKWINSVYMKKLDDDKYLEFVKSFINTNKFDITSKSEAWLNHLLLLYKKELEYAEQINDHLDLFFNKNTLDNNTIDVLNNLTNYKNVVEIFKNQINDLKDWTIENIKQIIKNTSTLANVKGKDLFMPIRIFATKSEHGPSLADVIYLLGKTTVLNNINSLER"
ORIGIN
        1 ATGAGCTTCA GATTGCGTTA CGCGCCATCT CCTACAGGAT TTCTTCATAT CGGAAACACT
       61 CGGACCGCAT TGATGAACTA CCTGTTTGCA AAGCACTACA ATGGTTCGTT TATTGTGCGC
      121 ATTGAGGATA CGGACCTGAC GAGAAATGTC GATGGTGCCA TTGAATCTCA ATTCGAGAAT
      181 CTTAACTGGT TAGGCATATT CCCGGATGAG TCAATCTTTA ATGTCGGGGA CCAGAAATAC
      241 GGGAAGTATA TGCAATCCCA GAAGTTTGAC AGATATAAAC AGTTGGCGGA ACAATTAGTT
      301 GACCAAAACA AAGCCTACAG ATGTTTCTGT ACATCCGAGG AGCTGGAGAA GGACTATGAG
      361 GAACAAACCT CTAAGGGTAT TATTGCCACG AAGTATTCGC AGAAATGTTT GTTCCTGACC
      421 CAAGATCAAA TACAGAAAAA CCTTGAGAAC AAGAAAGAAT ACTCCATCCG CTTCAAGGTG
      481 CCCGAGAACA AGACTTGGAC GATCAATGAC ATAGTAAGAG GCGACGTTAG CTTTGACTCA
      541 AAAGACCTGG GAGACTTCGT AATTTTAAAG AGCAATGGGG TAGCCACCTA TAATTTTGCC
      601 GTGGTTATTG ATGACTATGA TATGCAGATC ACTCACGTAT TAAGAGGCGA GGAACACATA
      661 TCAAACACCC CACGGCAAAT GATGATATAT GACGCTTTTA ATTGGAATTA TCCCAAGTTC
      721 GGCCACCTGA CGCTGATTGT CGATAACACC GGCAAAAAAC TGTCCAAGAG ATCAGGAAAT
      781 GCTCTGTTTT TCATAGAGCA ATACAAAAAG CAAGGATACC TGAGTCAAGC GATCTTCAAC
      841 TACATAGCAT TGTTGGGATG GAGCCCACCG GGCGAACAGG AAATACTGTC CCAAAACGAA
      901 CTTATTAAAA TCTTTGATGA AAAACGGTTT TCTAAATCCC CGTCTACCTT TGATATGGTT
      961 AAAATGAAGT GGATTAACTC TGTGTATATG AAGAAATTAG ATGACGACAA GTACCTTGAG
     1021 TTTGTCAAGT CATTTATTAA TACAAATAAG TTTGACATAA CGTCAAAAAG CGAGGCCTGG
     1081 CTTAATCACC TGTTACTGTT GTACAAAAAA GAGCTGGAGT ATGCTGAACA AATCAATGAT
     1141 CACCTGGATT TATTTTTTAA TAAGAACACT TTGGACAATA ACACCATTGA CGTGCTGAAC
     1201 AATCTTACCA ACTATAAGAA CGTCGTTGAG ATTTTTAAGA ATCAGATCAA CGACCTTAAA
     1261 GACTGGACGA TCGAAAACAT AAAGCAAATA ATAAAAAACA CCTCGACGTT GGCGAATGTC
     1321 AAAGGAAAAG ACTTATTCAT GCCGATACGC ATTTTTGCTA CGAAATCCGA GCACGGCCCT
     1381 AGCCTGGCTG ATGTTATATA CTTGTTAGGT AAGACAACCG TATTGAATAA CATAAATTCG
     1441 CTGGAGCGCT GAAGAGCTTA GAGACCGGTA CTAGTTCCTG CAGGGCTCGA GGTGTCAATC
     1501 GTCGGAGCCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG
     1561 GGTTTTTTGC ATGGTCATAG CTGTTTCCTG AGAGCTTGGC AGGTGATGAC ACACATTAAC
     1621 AAATTTCGTG AGGAGTCTCC AGAAGAATGC CATTAATTTC CATAGGCTCC GCCCCCCTGA
     1681 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG
     1741 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT
     1801 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG
     1861 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC
     1921 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT
     1981 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA
     2041 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC
     2101 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC
     2161 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT
     2221 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC
     2281 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT
     2341 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA
     2401 AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT
     2461 ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG
     2521 CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAA CCACGCTCAC CGGCTCCAGA
     2581 TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT
     2641 ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT
     2701 TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT
     2761 TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT
     2821 GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC
     2881 CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC
     2941 CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT
     3001 GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG
     3061 AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT
     3121 ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC
     3181 TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA
     3241 GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG
     3301 AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA
     3361 TAAACAAATA GGGGTTCCGC GCACCTGCAC CAGTCAGTAA AACGACGGCC AGTAGTCAAA
     3421 AGCCTCCGAC CGGAGGCTTT TGACTTGGTC AGGTGGAGTG AATTCGCGGC CGCTTCTAGA
     3481 GGCTCTTCGT AATTACGAAG AGGTCTCTA
//