LOCUS       Exported     3656 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3656
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1669..1716
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3544..3560
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3561..3592
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0129"
                     /note="PyrG: CTP synthase"
                     /translation="MAKFIFVTGGVVSGLGKGITASSIGALLKASGLKVFMQKFDPYLNVDPGTMSPYQHGEVFVTKDGGETDLDLGHYERFIDEELTKLSSTTSGKIYLSVIQGERKGVNSGKTIQVVPHITDAIKQKVYQAAEQSQADVIISEIGGTVGDIESQPFIEAIRQIRLEQGKENVMFVHVVLLLWLAASKEYKTKPIQNSVKAMASLGIQPDVIVCRSDSSSPKDIKEKISLFCNVPITNIIDAIDQDSIYRVPLALAKQNIQDIIIEQLQLKAKNIDLSLWKQFNKKIDSSTEEIEISFVGKYIELQDAYLSVLESLKIAGWEFNKKIKIRWVQADKLDESNYKEILKNSQGILVPGGFGKRGIEGMMLASRYARENDIPYLGICLGMQIATISIARDLLNWTDADSTEFNKNTTHPIFDYIKGIDRDNIGGTLRLGTMVTKLEKDSLVSKLYNSDVALERHRHRYEFNNEYKKDLESVGLRFSGIYEEKNLVEVVEMPSLKFFVASQFHPEFTSRPNKPTPLFRGFIKAIVENNK"
     primer_bind     complement(1721..1737)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1804..2392)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(2563..3423)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3424..3528)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /translation="MAKFIFVTGGVVSGLGKGITASSIGALLKASGLKVFMQKFDPYLNVDPGTMSPYQHGEVFVTKDGGETDLDLGHYERFIDEELTKLSSTTSGKIYLSVIQGERKGVNSGKTIQVVPHITDAIKQKVYQAAEQSQADVIISEIGGTVGDIESQPFIEAIRQIRLEQGKENVMFVHVVLLLWLAASKEYKTKPIQNSVKAMASLGIQPDVIVCRSDSSSPKDIKEKISLFCNVPITNIIDAIDQDSIYRVPLALAKQNIQDIIIEQLQLKAKNIDLSLWKQFNKKIDSSTEEIEISFVGKYIELQDAYLSVLESLKIAGWEFNKKIKIRWVQADKLDESNYKEILKNSQGILVPGGFGKRGIEGMMLASRYARENDIPYLGICLGMQIATISIARDLLNWTDADSTEFNKNTTHPIFDYIKGIDRDNIGGTLRLGTMVTKLEKDSLVSKLYNSDVALERHRHRYEFNNEYKKDLESVGLRFSGIYEEKNLVEVVEMPSLKFFVASQFHPEFTSRPNKPTPLFRGFIKAIVENNK"
                     /label="MMSYN1_0129"
                     /note="PyrG: CTP synthase"
ORIGIN
        1 ATGGCAAAAT TCATTTTTGT CACTGGTGGG GTTGTCTCAG GGTTAGGTAA AGGTATTACC
       61 GCTAGTTCTA TCGGGGCTTT GTTGAAGGCG AGCGGCTTAA AAGTTTTCAT GCAAAAGTTT
      121 GATCCATACC TTAACGTCGA CCCAGGAACC ATGAGCCCTT ACCAACACGG GGAAGTATTT
      181 GTTACCAAGG ATGGAGGGGA AACGGATTTG GATTTAGGAC ATTACGAACG TTTCATCGAT
      241 GAAGAACTTA CTAAACTTAG TTCTACCACA TCCGGGAAGA TTTATCTGTC TGTAATTCAG
      301 GGTGAACGCA AAGGGGTAAA TTCAGGTAAG ACCATCCAAG TAGTGCCTCA TATTACCGAC
      361 GCCATCAAAC AGAAGGTTTA CCAGGCGGCT GAACAATCTC AGGCAGACGT CATCATCTCG
      421 GAAATTGGGG GAACAGTAGG CGATATTGAG AGTCAACCCT TTATCGAGGC GATTCGCCAG
      481 ATTCGCTTAG AGCAGGGTAA AGAAAACGTG ATGTTCGTTC ACGTTGTGTT GTTGTTATGG
      541 TTAGCCGCTT CGAAAGAGTA TAAAACTAAG CCCATCCAGA ACTCCGTAAA GGCAATGGCA
      601 TCTCTTGGGA TTCAGCCCGA TGTAATTGTA TGCCGTAGTG ATTCTTCTTC TCCGAAGGAT
      661 ATTAAGGAGA AAATTAGCCT GTTTTGTAAC GTGCCAATTA CAAACATCAT CGACGCGATT
      721 GACCAGGATA GTATCTATCG TGTGCCGCTG GCCCTTGCCA AGCAGAACAT TCAAGACATT
      781 ATCATCGAGC AACTTCAGCT GAAGGCCAAG AACATTGATT TATCTCTGTG GAAACAATTC
      841 AACAAAAAGA TCGATAGCTC AACTGAGGAA ATTGAGATCT CCTTTGTAGG CAAGTACATT
      901 GAACTGCAAG ACGCGTATCT TTCCGTGCTG GAGAGCCTGA AGATCGCAGG GTGGGAGTTC
      961 AACAAGAAAA TTAAAATTCG CTGGGTGCAA GCTGACAAAT TAGACGAATC AAACTACAAG
     1021 GAAATTTTGA AAAACAGCCA AGGGATTTTG GTACCCGGCG GGTTTGGAAA GCGCGGTATC
     1081 GAGGGAATGA TGTTAGCTTC TCGTTATGCT CGCGAGAATG ACATTCCATA TCTTGGGATT
     1141 TGCTTGGGCA TGCAGATTGC AACGATTAGC ATTGCGCGTG ACCTGTTGAA TTGGACAGAC
     1201 GCCGATAGTA CCGAATTTAA CAAAAACACT ACTCACCCAA TTTTTGATTA CATCAAAGGT
     1261 ATTGATCGTG ACAACATTGG AGGGACGCTT CGCTTGGGCA CGATGGTTAC AAAGCTTGAG
     1321 AAGGATAGCT TAGTCTCAAA ATTGTATAAC AGCGACGTTG CATTAGAACG CCACCGTCAT
     1381 CGTTATGAGT TCAACAATGA GTATAAGAAA GATTTGGAGT CAGTTGGGTT ACGCTTTAGC
     1441 GGGATCTACG AAGAGAAAAA CTTAGTAGAG GTAGTTGAAA TGCCCAGTTT AAAATTTTTC
     1501 GTCGCGAGCC AGTTTCATCC TGAGTTTACT TCTCGCCCGA ATAAACCCAC TCCACTTTTT
     1561 CGTGGTTTTA TCAAAGCTAT TGTAGAGAAT AACAAGTGAA GAGCTTAGAG ACCGGTACTA
     1621 GTTCCTGCAG GGCTCGAGGT GTCAATCGTC GGAGCCGCTG AGCAATAACT AGCATAACCC
     1681 CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCATG GTCATAGCTG TTTCCTGAGA
     1741 GCTTGGCAGG TGATGACACA CATTAACAAA TTTCGTGAGG AGTCTCCAGA AGAATGCCAT
     1801 TAATTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG
     1861 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT
     1921 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG
     1981 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG
     2041 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG
     2101 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC
     2161 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG
     2221 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT
     2281 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG
     2341 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC
     2401 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT
     2461 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT
     2521 TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG
     2581 TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT
     2641 CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC
     2701 GCGAGAACCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC
     2761 CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG
     2821 GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC
     2881 AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG
     2941 ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC
     3001 TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT
     3061 GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC
     3121 AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT
     3181 ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC
     3241 TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC
     3301 TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA
     3361 AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT
     3421 CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG
     3481 ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CCTGCACCAG
     3541 TCAGTAAAAC GACGGCCAGT AGTCAAAAGC CTCCGACCGG AGGCTTTTGA CTTGGTCAGG
     3601 TGGAGTGAAT TCGCGGCCGC TTCTAGAGGC TCTTCGTAAT TACGAAGAGG TCTCTA
//