LOCUS       Exported     2951 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2951
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      964..1011
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2839..2855
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2856..2887
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0131"
                     /note=""
                     /translation="MPKLYHKKLVNAKQMVIDAHRHRYAIGHFNINNLEWTKAILEAAEASKTPVIIATSEGAIKYMGGVNTVVGMVNGLLDYLNITVPVALHLDHGQSLEMAKKCILAGYSSVMFDGSHFPYAENLEMTKELIKFAEEYEVSVEAEIGSIGGEEDGVIGQGELGDPLQAEEISKTGITMLAAGIGNIHGKYPSWWQSLSFDTLERLQKACKMPMVLHGGSGIPQEQVKKAISMGIAKINVNTELQLAFRDATRKYVEERKDLDDAKKGFDPRKLLKPGYDALKTTFLELTNWFGCQGKAK"
     primer_bind     complement(1016..1032)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1099..1687)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1858..2718)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2719..2823)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0131"
                     /note=""
                     /translation="MPKLYHKKLVNAKQMVIDAHRHRYAIGHFNINNLEWTKAILEAAEASKTPVIIATSEGAIKYMGGVNTVVGMVNGLLDYLNITVPVALHLDHGQSLEMAKKCILAGYSSVMFDGSHFPYAENLEMTKELIKFAEEYEVSVEAEIGSIGGEEDGVIGQGELGDPLQAEEISKTGITMLAAGIGNIHGKYPSWWQSLSFDTLERLQKACKMPMVLHGGSGIPQEQVKKAISMGIAKINVNTELQLAFRDATRKYVEERKDLDDAKKGFDPRKLLKPGYDALKTTFLELTNWFGCQGKAK"
ORIGIN
        1 ATGCCGAAAC TGTATCATAA AAAACTGGTG AACGCGAAAC AGATGGTGAT TGATGCGCAC
       61 CGCCACCGCT ATGCGATTGG CCATTTTAAC ATTAACAACC TGGAATGGAC CAAAGCGATT
      121 CTGGAAGCGG CGGAAGCGAG CAAAACCCCG GTGATTATTG CGACCAGCGA AGGCGCGATT
      181 AAATATATGG GCGGCGTGAA CACCGTGGTG GGCATGGTGA ACGGCCTGCT GGATTATCTG
      241 AACATTACCG TGCCGGTGGC GCTGCATCTG GATCATGGCC AGAGCCTGGA AATGGCGAAA
      301 AAATGCATTC TGGCGGGCTA TAGCAGCGTG ATGTTTGATG GCAGCCATTT TCCGTATGCG
      361 GAAAACCTGG AAATGACCAA AGAACTGATT AAATTTGCGG AAGAATATGA AGTGAGCGTG
      421 GAAGCGGAAA TTGGCAGCAT TGGCGGCGAA GAAGATGGCG TGATTGGCCA GGGCGAACTG
      481 GGCGATCCGC TGCAGGCGGA AGAAATTAGC AAAACCGGCA TTACCATGCT GGCGGCGGGC
      541 ATTGGCAACA TTCATGGCAA ATATCCGAGC TGGTGGCAGA GCCTGAGCTT TGATACCCTG
      601 GAACGCCTGC AGAAAGCGTG CAAAATGCCG ATGGTGCTGC ATGGCGGCAG CGGCATTCCG
      661 CAGGAACAGG TGAAAAAAGC GATTAGCATG GGCATTGCGA AAATTAACGT GAACACCGAA
      721 CTGCAGCTGG CGTTTCGGGA TGCGACCCGC AAATATGTGG AAGAACGCAA AGATCTGGAT
      781 GATGCGAAAA AAGGCTTTGA TCCGCGCAAA CTGCTGAAAC CGGGCTATGA TGCGCTGAAA
      841 ACCACCTTTC TGGAACTGAC CAACTGGTTT GGCTGCCAGG GCAAAGCGAA GTGAAGAGCT
      901 TAGAGACCGG TACTAGTTCC TGCAGGGCTC GAGGTGTCAA TCGTCGGAGC CGCTGAGCAA
      961 TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCATGGTCAT
     1021 AGCTGTTTCC TGAGAGCTTG GCAGGTGATG ACACACATTA ACAAATTTCG TGAGGAGTCT
     1081 CCAGAAGAAT GCCATTAATT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG
     1141 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC
     1201 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC
     1261 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC
     1321 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG
     1381 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC
     1441 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA
     1501 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC
     1561 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC
     1621 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG
     1681 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC
     1741 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA
     1801 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA
     1861 CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT
     1921 TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG
     1981 TGCTGCAATG ATACCGCGAG AACCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA
     2041 GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC
     2101 TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT
     2161 TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG
     2221 CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT
     2281 TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT
     2341 GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT
     2401 GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC
     2461 TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT
     2521 CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG
     2581 TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT
     2641 TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG
     2701 GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA
     2761 TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC
     2821 GCGCACCTGC ACCAGTCAGT AAAACGACGG CCAGTAGTCA AAAGCCTCCG ACCGGAGGCT
     2881 TTTGACTTGG TCAGGTGGAG TGAATTCGCG GCCGCTTCTA GAGGCTCTTC GTAATTACGA
     2941 AGAGGTCTCT A
//