LOCUS       Exported     4297 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4297
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2010..4283
                     /label="MMSYN1_0133"
                     /note=""
                     /translation="MNNLIVLKGKFEPGKNTKKPNSPQIPKTSIIKLEDCYRILDQLIKASSFWKEQKIDINPIINVKYKRIISKSNRVSYLLLKSLQKNNEHIIGSSFLDELVEKKIVKKQVITYCLTQKDLQEAIKRLDTITNILKKTHFKRIDNNLINLIANEQYLPIKKEIQKYEFLSRTAFISTLVDLNYIEEIFIKTTHIDNNVDSVVTLYDTGIKAIDLLNKLDINVNMSDFIDDYTLFLDRNQYNELKTKAPFLISMSVDDLTKFIIDDKQEEITKNDIISIPDPTNEPIVGVIDTMFCKDVYFSKWVDFRKEVSDDILLDSKDYQHGTQVSSIIVDGPSFNKKLEDGCGRFRVRHFGVMAHSSGNVFSLFKKIKSIVINNLDIKVWNLSLGSIREVSSNYISLLGSLLDQLQYENDVIFIVAGTNDNECKQKIVGSPADSINSIVVNSVDFKNKPANYSRKGPVLTYFNKPDISYYGGVDNNKITVCGCYGEAKVQGTSFAAPWITRKVAYLIYKMNYSKEEAKALIIDSAIKFDKQKDNNRDLIGYGVVPIHINEILQSKNTDIKVLLSYNTKAYYTYNFNLPVPTKENKFPFIAKLTFAYFAESQRSQGVDYTQDELDIQFGPIDNKSESINDINENNQSSSSSNAYIYEYEARKMFAKWNTVKSIIKWSKTNKGKKRQFIKTTNNRWGIRVIRKTRTDNINNKSIKFSLVITFRSIDNKDRIEEFISLCNKSGYWVASKVQIDNKIDIHGKSNEYLDFE"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGAATAACTT GATTGTGCTT AAAGGCAAGT
     2041 TTGAACCAGG CAAGAACACA AAGAAACCAA ATTCGCCTCA GATCCCTAAA ACCTCGATCA
     2101 TTAAGTTAGA GGACTGTTAT CGCATTCTTG ACCAACTTAT CAAAGCCTCA AGCTTCTGGA
     2161 AGGAACAGAA GATTGATATT AATCCCATCA TCAATGTAAA ATATAAACGC ATCATTTCAA
     2221 AGAGTAATCG CGTATCTTAT TTGCTTCTGA AATCATTGCA GAAAAACAAT GAACATATTA
     2281 TTGGCAGTTC TTTTCTGGAT GAGTTAGTAG AGAAAAAGAT TGTCAAAAAG CAAGTGATTA
     2341 CATACTGCTT GACGCAGAAA GACCTTCAGG AGGCAATCAA GCGTTTGGAT ACCATTACTA
     2401 ATATCCTGAA AAAGACTCAC TTTAAACGTA TTGACAACAA TTTGATCAAC CTTATTGCAA
     2461 ATGAACAATA TTTACCCATC AAGAAAGAGA TTCAAAAGTA TGAGTTTTTA AGCCGTACAG
     2521 CTTTCATCAG CACCTTGGTT GACCTGAACT ACATTGAGGA GATCTTTATC AAAACTACAC
     2581 ACATCGACAA TAATGTCGAT TCCGTAGTTA CTCTGTACGA TACAGGGATC AAGGCTATCG
     2641 ACTTACTTAA CAAACTGGAC ATTAACGTCA ATATGTCAGA TTTTATTGAT GATTATACGT
     2701 TATTTTTGGA CCGTAACCAG TACAATGAAC TTAAGACGAA GGCCCCTTTC CTTATTAGTA
     2761 TGTCGGTTGA TGACTTGACT AAGTTTATCA TCGACGATAA GCAGGAAGAG ATCACGAAGA
     2821 ATGATATTAT TAGCATCCCA GACCCAACTA ATGAACCCAT CGTCGGCGTG ATTGATACCA
     2881 TGTTCTGCAA GGATGTGTAT TTCTCAAAAT GGGTCGATTT CCGTAAAGAG GTGTCGGACG
     2941 ACATTCTGTT GGATTCTAAG GACTACCAGC ATGGTACTCA GGTGTCCTCC ATCATCGTAG
     3001 ATGGGCCGTC ATTCAATAAG AAACTTGAAG ATGGGTGTGG TCGCTTCCGT GTCCGTCACT
     3061 TTGGAGTGAT GGCCCACAGC TCGGGTAACG TTTTTAGCTT ATTTAAAAAG ATCAAGAGCA
     3121 TTGTTATCAA TAACCTGGAT ATTAAGGTTT GGAACTTGTC GTTGGGATCT ATTCGCGAAG
     3181 TATCATCTAA TTACATCTCT TTGCTGGGCT CTCTGCTTGA CCAACTTCAA TATGAGAACG
     3241 ATGTAATTTT TATCGTGGCA GGGACAAATG ATAATGAATG TAAACAAAAG ATCGTCGGCT
     3301 CGCCAGCGGA CTCTATTAAT AGCATCGTCG TCAACTCAGT AGACTTCAAA AATAAACCAG
     3361 CCAATTACTC ACGCAAAGGA CCGGTTCTTA CCTATTTCAA TAAACCAGAC ATCTCATACT
     3421 ACGGAGGTGT TGACAATAAC AAAATCACGG TCTGTGGGTG CTACGGCGAA GCAAAGGTCC
     3481 AGGGAACTAG TTTTGCAGCA CCCTGGATTA CCCGCAAAGT CGCGTACTTG ATTTACAAAA
     3541 TGAACTATTC CAAAGAAGAA GCAAAGGCTT TAATTATCGA CAGCGCGATC AAGTTCGATA
     3601 AACAGAAGGA CAACAACCGT GATCTTATCG GATACGGCGT CGTTCCGATT CACATTAACG
     3661 AAATCCTTCA GTCCAAGAAT ACTGACATTA AAGTGTTGTT ATCTTACAAT ACTAAGGCGT
     3721 ACTATACTTA TAACTTCAAC TTACCTGTAC CAACCAAAGA GAATAAATTT CCATTTATCG
     3781 CAAAATTGAC ATTCGCATAT TTCGCTGAGA GTCAACGCAG CCAAGGTGTG GACTACACCC
     3841 AAGATGAACT TGATATCCAA TTCGGTCCTA TTGATAATAA AAGTGAAAGT ATCAACGACA
     3901 TCAACGAGAA TAACCAAAGC TCGTCCTCAT CGAATGCATA CATCTATGAG TACGAGGCTC
     3961 GTAAAATGTT CGCCAAATGG AATACGGTGA AGTCAATTAT TAAATGGTCT AAAACTAATA
     4021 AGGGTAAAAA ACGTCAATTT ATTAAAACCA CTAATAACCG TTGGGGTATT CGTGTTATCC
     4081 GTAAAACTCG TACTGACAAC ATCAACAATA AGTCAATTAA ATTCTCGCTG GTTATCACGT
     4141 TCCGTTCTAT TGACAATAAA GACCGCATTG AAGAGTTTAT CAGTCTTTGT AACAAAAGCG
     4201 GGTACTGGGT TGCAAGCAAG GTGCAAATCG ATAATAAAAT CGACATTCAT GGTAAGTCTA
     4261 ATGAATATCT GGACTTCGAG TGAAGAGCTT AGAGACC
//