LOCUS       Exported     3922 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3922
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             2010..3908
                     /label="MMSYN1_0138"
                     /note=""
                     /translation="MSYKIKELTFRSKNPSLNKVDFIADDGQIVDIVIDNKKEMDFFIKVLLGKKKNSSGRFQIDDFDIINRAYTKKHVEFIKRDTWFQRIIPSKWVLVLSLLFDQNFLKTASNKYLEKKYEYLSLVASKGEANDKKLRQNIDNLISKHIISKTREEQKALNESINTQKKHNQEKFLAIAEKWPIQIRLLSKAVENLKTEIKTATLMLMFQQTLWDNVYALDELRDNCSCEYNAKHSSNKKLKKSWKKFAYQQTYYAVHKQLRIISTKIADLRLSIFRQQKRLKQFEKQLDFEFKKYLRSLLSSTTNKTEKKDINNNWEQTKKYFTDWKNANKNTLNDLEKQQIELHIEPIRKTTQQLGETINFLIHQYHERVLSDELEYIDKRRFLKQKKEKKKEIKSVFKQAVEKMSTSVDNYNIKFEWFIKSSVKYLSLNIVYLKILKAINLKKRNIIFFNITKHLSEKELLQLFETIKSIQQHHPLMTFIFLNDSINDVYDLNKSIYYTNNKLELKEMLAVDIFDSLLKKQDNNINKISYKKINENEIKFLNESTWVLTNYNLKDTGYISFNPLKISTEPKKRINLLLSATVIKSKKFIDKSMYFALTEEKEKIYFYDRTNLYKDNDEIVLYISKDSISSIN"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGTCCTACAA GATCAAAGAG CTGACATTTC
     2041 GCAGCAAAAA CCCGTCCTTA AATAAAGTCG ACTTCATTGC TGATGACGGA CAGATCGTGG
     2101 ATATCGTTAT TGATAACAAA AAGGAAATGG ACTTTTTCAT CAAGGTGTTA TTGGGAAAGA
     2161 AGAAGAACTC GAGCGGTCGT TTCCAAATTG ACGACTTTGA CATCATTAAC CGTGCATATA
     2221 CGAAAAAGCA CGTAGAATTT ATCAAACGCG ACACCTGGTT CCAGCGTATC ATTCCTAGCA
     2281 AATGGGTTCT GGTCCTGTCC CTTCTTTTCG ACCAGAATTT TTTGAAAACC GCATCAAATA
     2341 AATACTTGGA GAAAAAGTAC GAGTACCTGA GTCTGGTCGC AAGTAAGGGG GAGGCCAATG
     2401 ATAAAAAGTT GCGCCAGAAC ATTGATAATT TGATTAGTAA ACACATCATT AGCAAGACCC
     2461 GCGAAGAACA AAAAGCTTTG AACGAGTCCA TTAACACCCA AAAGAAGCAC AATCAAGAAA
     2521 AATTCTTAGC TATCGCAGAA AAATGGCCCA TTCAAATCCG CTTGCTTAGC AAAGCAGTTG
     2581 AGAATTTAAA AACGGAAATC AAAACTGCTA CTTTAATGTT GATGTTCCAA CAGACATTAT
     2641 GGGATAACGT ATACGCATTG GATGAGCTTC GTGACAACTG CTCATGCGAA TATAACGCAA
     2701 AGCATTCAAG CAACAAAAAG CTTAAGAAAT CCTGGAAGAA ATTTGCGTAC CAGCAAACAT
     2761 ACTACGCCGT ACATAAACAA TTGCGCATTA TTTCCACCAA AATCGCGGAC TTACGTTTGT
     2821 CTATTTTCCG TCAGCAGAAA CGCTTAAAAC AATTCGAAAA GCAACTGGAT TTCGAGTTTA
     2881 AGAAATATCT TCGTTCCTTG CTGTCATCCA CGACGAATAA AACCGAGAAA AAGGACATTA
     2941 ATAATAACTG GGAGCAGACT AAGAAATACT TTACGGATTG GAAAAATGCG AATAAGAACA
     3001 CTCTGAATGA TCTTGAAAAA CAGCAGATCG AATTACACAT CGAACCTATC CGCAAAACTA
     3061 CGCAGCAACT TGGGGAAACC ATTAATTTTT TAATTCATCA ATACCACGAG CGTGTATTGT
     3121 CGGACGAACT TGAATACATC GACAAACGCC GCTTCTTGAA GCAGAAAAAG GAGAAGAAGA
     3181 AAGAGATTAA ATCGGTATTT AAGCAGGCCG TCGAGAAAAT GTCGACATCC GTCGATAACT
     3241 ATAATATTAA GTTTGAATGG TTTATCAAAT CGTCCGTTAA ATATTTGAGC CTTAATATCG
     3301 TCTACCTGAA GATCTTAAAG GCGATCAATT TGAAAAAACG TAACATCATC TTCTTTAATA
     3361 TTACAAAACA TTTAAGTGAG AAAGAACTTT TGCAGCTTTT CGAAACAATT AAGAGCATCC
     3421 AACAACATCA TCCACTGATG ACTTTCATCT TTTTAAATGA CAGTATCAAT GACGTTTACG
     3481 ACTTAAATAA GTCGATTTAT TACACCAATA ACAAATTGGA ACTGAAGGAA ATGCTTGCGG
     3541 TGGACATCTT TGACTCATTA CTGAAAAAAC AGGATAACAA CATTAACAAG ATCTCTTATA
     3601 AGAAGATTAA TGAAAACGAG ATCAAGTTCT TAAATGAATC GACATGGGTC TTGACCAATT
     3661 ATAACTTAAA GGATACAGGG TACATCTCTT TTAATCCTCT TAAAATTTCA ACAGAGCCCA
     3721 AGAAGCGTAT TAACCTTTTA CTTTCGGCCA CAGTAATTAA GTCCAAGAAA TTCATCGACA
     3781 AATCCATGTA CTTCGCACTG ACGGAAGAAA AAGAGAAGAT TTATTTCTAC GATCGTACGA
     3841 ACCTGTATAA GGACAACGAT GAGATCGTGC TGTACATCTC AAAGGACTCC ATCAGTTCAA
     3901 TCAACTGAAG AGCTTAGAGA CC
//