LOCUS       Exported     3152 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3152
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1165..1212
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3040..3056
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      3057..3088
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0141"
                     /note="Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA."
                     /translation="MNAKTYEALETMQKRLLQILKDLEDENILKDIKKFTELNKEKSNLEEVVEKFVEYKQAVEHIKDAKVILENEKDQELIELAKIELDENNDKVEHLQQVIEEMLLPKDPNDDKNVIVEIRGAAGGDEANIFAGDLLRMYKLYAETQNWKINILEASVGEAGGYSQVVFMIKGDRVYSKLKFESGAHRVQRVPKTEAKGRIQTSTATVAVLPEMSEVEIEIRSNDLRIDTYRASGAGGQHVNTTDSAVRITHLPTGIVVTSQDGRSQHDNKDIAMTMLRAKVYEAEVEKQQAQADATRKNAVGTGARSEKIRTYNYPQNRVTDHRVGLTLNKLDQVMEGNIDEFIIALINEEQRQKVAEQLEKNNE"
     primer_bind     complement(1217..1233)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1300..1888)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2059..2919)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2920..3024)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0141"
                     /note="Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA."
                     /translation="MNAKTYEALETMQKRLLQILKDLEDENILKDIKKFTELNKEKSNLEEVVEKFVEYKQAVEHIKDAKVILENEKDQELIELAKIELDENNDKVEHLQQVIEEMLLPKDPNDDKNVIVEIRGAAGGDEANIFAGDLLRMYKLYAETQNWKINILEASVGEAGGYSQVVFMIKGDRVYSKLKFESGAHRVQRVPKTEAKGRIQTSTATVAVLPEMSEVEIEIRSNDLRIDTYRASGAGGQHVNTTDSAVRITHLPTGIVVTSQDGRSQHDNKDIAMTMLRAKVYEAEVEKQQAQADATRKNAVGTGARSEKIRTYNYPQNRVTDHRVGLTLNKLDQVMEGNIDEFIIALINEEQRQKVAEQLEKNNE"
ORIGIN
        1 ATGAACGCAA AGACGTATGA AGCTCTTGAG ACAATGCAGA AACGCTTACT GCAAATCCTG
       61 AAAGACCTGG AGGACGAAAA TATTTTGAAG GACATTAAAA AGTTTACGGA ACTGAATAAG
      121 GAGAAGTCCA ACTTAGAAGA GGTTGTGGAA AAATTCGTAG AGTATAAGCA GGCCGTGGAA
      181 CACATCAAAG ATGCGAAGGT CATTTTAGAG AACGAAAAAG ACCAGGAACT TATCGAATTG
      241 GCAAAAATTG AGTTAGATGA AAACAATGAC AAAGTGGAAC ATCTGCAGCA AGTCATTGAG
      301 GAGATGCTGC TGCCGAAAGA CCCGAATGAC GACAAAAATG TTATCGTGGA GATTCGTGGG
      361 GCCGCCGGTG GAGATGAGGC TAATATCTTT GCGGGTGACT TGCTTCGTAT GTACAAGTTA
      421 TACGCGGAGA CACAAAACTG GAAGATCAAT ATTCTGGAGG CCTCAGTTGG CGAGGCAGGC
      481 GGCTATTCGC AAGTGGTTTT TATGATCAAG GGCGACCGTG TCTATTCGAA GTTAAAGTTT
      541 GAGTCGGGAG CTCACCGCGT TCAACGCGTT CCCAAGACCG AAGCGAAAGG ACGCATCCAA
      601 ACCAGCACCG CCACTGTTGC CGTTCTTCCG GAGATGTCCG AGGTCGAAAT TGAAATCCGT
      661 AGCAATGATC TGCGTATTGA CACTTATCGT GCAAGTGGAG CGGGGGGACA ACATGTGAAT
      721 ACCACAGACT CCGCAGTGCG TATCACACAC CTTCCTACGG GCATCGTTGT AACAAGTCAG
      781 GACGGGCGTA GCCAACATGA TAATAAGGAT ATTGCTATGA CCATGCTGCG CGCCAAGGTC
      841 TACGAAGCAG AGGTAGAAAA ACAGCAAGCA CAAGCGGACG CTACCCGCAA AAACGCCGTT
      901 GGGACCGGGG CACGTTCTGA AAAAATCCGC ACGTACAACT ACCCACAGAA CCGCGTTACG
      961 GATCACCGCG TGGGTCTGAC TCTGAATAAA CTGGACCAAG TTATGGAGGG TAATATCGAT
     1021 GAGTTCATCA TTGCGCTGAT CAATGAGGAG CAGCGTCAGA AGGTGGCGGA ACAATTAGAG
     1081 AAAAACAACG AGTGAAGAGC TTAGAGACCG GTACTAGTTC CTGCAGGGCT CGAGGTGTCA
     1141 ATCGTCGGAG CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG
     1201 AGGGGTTTTT TGCATGGTCA TAGCTGTTTC CTGAGAGCTT GGCAGGTGAT GACACACATT
     1261 AACAAATTTC GTGAGGAGTC TCCAGAAGAA TGCCATTAAT TTCCATAGGC TCCGCCCCCC
     1321 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA
     1381 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC
     1441 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC
     1501 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA
     1561 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC
     1621 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG
     1681 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG
     1741 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG
     1801 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA
     1861 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA
     1921 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT
     1981 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA
     2041 GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG
     2101 TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA
     2161 GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GAACCACGCT CACCGGCTCC
     2221 AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC
     2281 TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC
     2341 AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC
     2401 GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC
     2461 CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT
     2521 GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC
     2581 ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG
     2641 TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG
     2701 CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT
     2761 CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC
     2821 ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA
     2881 AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA
     2941 TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA
     3001 AAATAAACAA ATAGGGGTTC CGCGCACCTG CACCAGTCAG TAAAACGACG GCCAGTAGTC
     3061 AAAAGCCTCC GACCGGAGGC TTTTGACTTG GTCAGGTGGA GTGAATTCGC GGCCGCTTCT
     3121 AGAGGCTCTT CGTAATTACG AAGAGGTCTC TA
//