LOCUS Exported 3152 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3152 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 1165..1212 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 3040..3056 /note="common sequencing primer, one of multiple similar variants" /label="M13 for" terminator 3057..3088 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 1..0 /label="MMSYN1_0141" /note="Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA." /translation="MNAKTYEALETMQKRLLQILKDLEDENILKDIKKFTELNKEKSNLEEVVEKFVEYKQAVEHIKDAKVILENEKDQELIELAKIELDENNDKVEHLQQVIEEMLLPKDPNDDKNVIVEIRGAAGGDEANIFAGDLLRMYKLYAETQNWKINILEASVGEAGGYSQVVFMIKGDRVYSKLKFESGAHRVQRVPKTEAKGRIQTSTATVAVLPEMSEVEIEIRSNDLRIDTYRASGAGGQHVNTTDSAVRITHLPTGIVVTSQDGRSQHDNKDIAMTMLRAKVYEAEVEKQQAQADATRKNAVGTGARSEKIRTYNYPQNRVTDHRVGLTLNKLDQVMEGNIDEFIIALINEEQRQKVAEQLEKNNE" primer_bind complement(1217..1233) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1300..1888) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2059..2919) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(2920..3024) /label="bla promoter" /note="ampicillin resistance gene promoter" CDS complement(1..0) /label="MMSYN1_0141" /note="Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA." /translation="MNAKTYEALETMQKRLLQILKDLEDENILKDIKKFTELNKEKSNLEEVVEKFVEYKQAVEHIKDAKVILENEKDQELIELAKIELDENNDKVEHLQQVIEEMLLPKDPNDDKNVIVEIRGAAGGDEANIFAGDLLRMYKLYAETQNWKINILEASVGEAGGYSQVVFMIKGDRVYSKLKFESGAHRVQRVPKTEAKGRIQTSTATVAVLPEMSEVEIEIRSNDLRIDTYRASGAGGQHVNTTDSAVRITHLPTGIVVTSQDGRSQHDNKDIAMTMLRAKVYEAEVEKQQAQADATRKNAVGTGARSEKIRTYNYPQNRVTDHRVGLTLNKLDQVMEGNIDEFIIALINEEQRQKVAEQLEKNNE" ORIGIN 1 ATGAACGCAA AGACGTATGA AGCTCTTGAG ACAATGCAGA AACGCTTACT GCAAATCCTG 61 AAAGACCTGG AGGACGAAAA TATTTTGAAG GACATTAAAA AGTTTACGGA ACTGAATAAG 121 GAGAAGTCCA ACTTAGAAGA GGTTGTGGAA AAATTCGTAG AGTATAAGCA GGCCGTGGAA 181 CACATCAAAG ATGCGAAGGT CATTTTAGAG AACGAAAAAG ACCAGGAACT TATCGAATTG 241 GCAAAAATTG AGTTAGATGA AAACAATGAC AAAGTGGAAC ATCTGCAGCA AGTCATTGAG 301 GAGATGCTGC TGCCGAAAGA CCCGAATGAC GACAAAAATG TTATCGTGGA GATTCGTGGG 361 GCCGCCGGTG GAGATGAGGC TAATATCTTT GCGGGTGACT TGCTTCGTAT GTACAAGTTA 421 TACGCGGAGA CACAAAACTG GAAGATCAAT ATTCTGGAGG CCTCAGTTGG CGAGGCAGGC 481 GGCTATTCGC AAGTGGTTTT TATGATCAAG GGCGACCGTG TCTATTCGAA GTTAAAGTTT 541 GAGTCGGGAG CTCACCGCGT TCAACGCGTT CCCAAGACCG AAGCGAAAGG ACGCATCCAA 601 ACCAGCACCG CCACTGTTGC CGTTCTTCCG GAGATGTCCG AGGTCGAAAT TGAAATCCGT 661 AGCAATGATC TGCGTATTGA CACTTATCGT GCAAGTGGAG CGGGGGGACA ACATGTGAAT 721 ACCACAGACT CCGCAGTGCG TATCACACAC CTTCCTACGG GCATCGTTGT AACAAGTCAG 781 GACGGGCGTA GCCAACATGA TAATAAGGAT ATTGCTATGA CCATGCTGCG CGCCAAGGTC 841 TACGAAGCAG AGGTAGAAAA ACAGCAAGCA CAAGCGGACG CTACCCGCAA AAACGCCGTT 901 GGGACCGGGG CACGTTCTGA AAAAATCCGC ACGTACAACT ACCCACAGAA CCGCGTTACG 961 GATCACCGCG TGGGTCTGAC TCTGAATAAA CTGGACCAAG TTATGGAGGG TAATATCGAT 1021 GAGTTCATCA TTGCGCTGAT CAATGAGGAG CAGCGTCAGA AGGTGGCGGA ACAATTAGAG 1081 AAAAACAACG AGTGAAGAGC TTAGAGACCG GTACTAGTTC CTGCAGGGCT CGAGGTGTCA 1141 ATCGTCGGAG CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG 1201 AGGGGTTTTT TGCATGGTCA TAGCTGTTTC CTGAGAGCTT GGCAGGTGAT GACACACATT 1261 AACAAATTTC GTGAGGAGTC TCCAGAAGAA TGCCATTAAT TTCCATAGGC TCCGCCCCCC 1321 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 1381 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 1441 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 1501 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 1561 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 1621 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 1681 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 1741 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 1801 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 1861 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 1921 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 1981 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 2041 GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG 2101 TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA 2161 GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GAACCACGCT CACCGGCTCC 2221 AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC 2281 TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC 2341 AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC 2401 GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC 2461 CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT 2521 GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC 2581 ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG 2641 TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG 2701 CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT 2761 CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC 2821 ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA 2881 AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA 2941 TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA 3001 AAATAAACAA ATAGGGGTTC CGCGCACCTG CACCAGTCAG TAAAACGACG GCCAGTAGTC 3061 AAAAGCCTCC GACCGGAGGC TTTTGACTTG GTCAGGTGGA GTGAATTCGC GGCCGCTTCT 3121 AGAGGCTCTT CGTAATTACG AAGAGGTCTC TA //