LOCUS       Exported     2906 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2906
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      919..966
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2794..2810
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2811..2842
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /translation="MNNTIFNVLNKIKNTNISLNKADVYHILEHIINKDYQWIISNLDYKLTKKQIYKIDQILDLLKQNYPLAYILKSKYFYSNNFFINKDVLIPRNESELIIDHASEFVKNNNDLLIVDLCTGSGCLGISCALLNDQNKVILTDISYKALKVANKNIKKFNLTNTSCLNGNFIDVLIKNNLKANLIICNPPYIDINDQNIDKNVIDFEPSIALFAPNKGLYFYEILIKNIDKILDTNKNFLIVLEFGWLQKDSIEQLLINNCLKYKWEFKKDYNDYWRNLIIKNF"
                     /label="MMSYN1_0142"
                     /note="Methylates the class 1 translation termination release factors RF1/PrfA and RF2/PrfB on the glutamine residue of the universally conserved GGQ motif."
     primer_bind     complement(971..987)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(1054..1642)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(1813..2673)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2674..2778)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0142"
                     /note="Methylates the class 1 translation termination release factors RF1/PrfA and RF2/PrfB on the glutamine residue of the universally conserved GGQ motif."
                     /translation="MNNTIFNVLNKIKNTNISLNKADVYHILEHIINKDYQWIISNLDYKLTKKQIYKIDQILDLLKQNYPLAYILKSKYFYSNNFFINKDVLIPRNESELIIDHASEFVKNNNDLLIVDLCTGSGCLGISCALLNDQNKVILTDISYKALKVANKNIKKFNLTNTSCLNGNFIDVLIKNNLKANLIICNPPYIDINDQNIDKNVIDFEPSIALFAPNKGLYFYEILIKNIDKILDTNKNFLIVLEFGWLQKDSIEQLLINNCLKYKWEFKKDYNDYWRNLIIKNF"
ORIGIN
        1 ATGAACAACA CAATTTTCAA CGTGTTGAAC AAGATCAAGA ACACAAATAT CTCTCTTAAT
       61 AAGGCTGATG TCTATCATAT TTTAGAGCAT ATTATTAATA AGGATTACCA ATGGATTATC
      121 TCGAATCTGG ACTATAAATT GACAAAGAAA CAGATCTATA AAATTGATCA GATCTTAGAT
      181 TTGCTTAAGC AGAACTATCC GCTGGCGTAC ATCTTGAAGT CCAAGTACTT CTACAGTAAT
      241 AATTTTTTCA TCAATAAAGA TGTTTTAATT CCACGCAATG AATCGGAACT GATCATTGAC
      301 CATGCCAGTG AGTTTGTCAA AAATAATAAT GACTTGCTGA TCGTCGATCT GTGCACTGGA
      361 TCAGGATGTC TGGGCATCTC CTGTGCATTA TTGAACGATC AGAACAAAGT CATCTTAACC
      421 GATATTTCTT ACAAAGCGCT TAAAGTCGCT AACAAAAACA TCAAAAAGTT CAATTTGACG
      481 AATACGTCCT GCCTGAATGG TAACTTTATT GACGTTCTTA TCAAAAATAA TCTTAAGGCT
      541 AATCTTATTA TCTGCAATCC CCCGTACATC GATATTAACG ACCAGAACAT CGACAAGAAT
      601 GTCATCGATT TTGAGCCCTC GATTGCACTG TTCGCTCCCA ATAAAGGCTT ATATTTTTAT
      661 GAGATTCTGA TTAAGAATAT TGACAAAATT TTAGATACCA ATAAAAACTT CCTTATCGTG
      721 TTAGAGTTCG GGTGGTTGCA GAAGGACTCG ATCGAACAGT TATTAATCAA TAATTGCCTG
      781 AAGTATAAAT GGGAGTTTAA GAAAGATTAT AACGATTATT GGCGCAACTT GATTATTAAA
      841 AATTTTTGAA GAGCTTAGAG ACCGGTACTA GTTCCTGCAG GGCTCGAGGT GTCAATCGTC
      901 GGAGCCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT
      961 TTTTTGCATG GTCATAGCTG TTTCCTGAGA GCTTGGCAGG TGATGACACA CATTAACAAA
     1021 TTTCGTGAGG AGTCTCCAGA AGAATGCCAT TAATTTCCAT AGGCTCCGCC CCCCTGACGA
     1081 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA
     1141 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC
     1201 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG
     1261 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC
     1321 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG
     1381 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT
     1441 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT
     1501 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG
     1561 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC
     1621 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA
     1681 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC
     1741 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC
     1801 TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT
     1861 TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT
     1921 ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGAACCA CGCTCACCGG CTCCAGATTT
     1981 ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC
     2041 CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA
     2101 TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG
     2161 TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT
     2221 GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC
     2281 AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT
     2341 AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG
     2401 GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC
     2461 TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC
     2521 GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT
     2581 TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG
     2641 AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG
     2701 CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA
     2761 ACAAATAGGG GTTCCGCGCA CCTGCACCAG TCAGTAAAAC GACGGCCAGT AGTCAAAAGC
     2821 CTCCGACCGG AGGCTTTTGA CTTGGTCAGG TGGAGTGAAT TCGCGGCCGC TTCTAGAGGC
     2881 TCTTCGTAAT TACGAAGAGG TCTCTA
//