LOCUS Exported 3496 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3496 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 38..85 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 1933..1949 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 1950..1981 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 2010..3482 /label="MMSYN1_0143" /note="membrane protein, putative" /translation="MYKNKNFKIKIINNKFSMRIKDIDPKIEQKNFSIYLKAILGLVVFLITLLPFYAYLHLIFKHESLSFYFANYSIISKYVDLPSKSQIWGLAISALVFMAIVITMFISFKALVNISNNKRYKQAIIALIIIFGILTILFQGISQYFYGYFQDFFNYQVISGLDNKISDFKKITTQFIEFEKNTSSIYNWIDVNNIWWIIFVQIFLMFVTSISLQNITFFEYEKNSEDKYINYFVQKNKVIYQNRIKLYVNNLFSFTDKTLSNWLIILVLMICFPILIYIVAISTRGSEKSLIYWTHQLPNLLKDYQNWNTIFDQYKNQLNLTKSSPLLILSSPIIFLGITLSTVLFLLTISIRGQKSSQLVLRTKFILLSILISLLILSIFISQLELHKLLVAWNTSNNEQIIGSNYIQAIKQITGQKVFENIDQKLFLLNNIDQKIDSIFNDRYIISVCISFLVVSTITGFCIILKGMLDKRLAIDFVKNQFKNKKLFRK" primer_bind complement(90..106) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(173..761) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(952..1812) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(1813..1917) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC 61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT 121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA 181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG 721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA 781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG 961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG 1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC 1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC 1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA 1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC 1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG 1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC 1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT 1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG 1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC 1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG 1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT 1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG 1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG 1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC 1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGTATAAAAA TAAGAACTTC AAGATCAAAA 2041 TTATCAATAA CAAATTCAGT ATGCGCATTA AGGACATTGA TCCTAAGATT GAACAAAAGA 2101 ATTTTTCTAT CTACCTTAAA GCAATCCTTG GCTTGGTAGT TTTCTTGATT ACGTTGCTTC 2161 CATTTTACGC ATACTTGCAT CTGATTTTCA AACACGAGTC GTTAAGCTTC TACTTTGCCA 2221 ATTACAGCAT CATCTCGAAG TATGTTGACT TGCCCTCAAA AAGCCAAATT TGGGGCTTAG 2281 CTATTTCGGC TTTGGTTTTC ATGGCGATCG TCATTACCAT GTTTATCAGC TTTAAGGCAT 2341 TGGTCAATAT CTCGAACAAC AAGCGCTACA AGCAAGCTAT TATCGCATTG ATTATCATCT 2401 TTGGTATTTT AACCATTCTT TTTCAGGGTA TTAGCCAGTA TTTCTATGGT TACTTCCAGG 2461 ACTTTTTTAA CTATCAAGTT ATTAGTGGTC TGGATAACAA AATTTCAGAT TTTAAAAAGA 2521 TTACCACGCA ATTTATCGAG TTCGAGAAAA ACACCTCGTC AATCTACAAT TGGATTGATG 2581 TAAATAATAT CTGGTGGATC ATTTTCGTGC AAATCTTTCT TATGTTCGTG ACTTCCATTT 2641 CGCTTCAAAA CATTACCTTC TTTGAGTACG AGAAGAACTC CGAGGACAAG TACATCAACT 2701 ACTTTGTCCA GAAGAACAAG GTTATTTACC AAAACCGTAT CAAACTGTAC GTTAACAACT 2761 TATTCAGTTT TACGGATAAG ACCCTTTCAA ACTGGCTGAT CATTCTTGTG TTGATGATCT 2821 GCTTTCCCAT CCTGATTTAC ATTGTCGCTA TCAGCACCCG CGGATCGGAG AAATCTTTAA 2881 TTTACTGGAC ACATCAGCTG CCCAATTTAC TGAAAGACTA TCAGAATTGG AATACCATTT 2941 TTGACCAGTA CAAAAACCAG CTTAACCTGA CAAAGAGCTC GCCATTACTT ATCTTGTCCT 3001 CGCCCATTAT TTTCCTTGGC ATCACCTTGT CAACGGTTTT GTTTTTATTA ACTATTAGCA 3061 TCCGCGGACA GAAGTCAAGC CAACTTGTAC TGCGTACCAA ATTCATTTTG TTAAGCATCC 3121 TGATCTCCTT GCTTATCTTA TCAATTTTTA TTTCGCAATT AGAACTGCAT AAATTGTTAG 3181 TCGCATGGAA CACTTCAAAC AACGAGCAAA TCATCGGAAG TAACTATATT CAGGCAATTA 3241 AACAAATTAC GGGGCAAAAA GTCTTTGAGA ACATTGACCA AAAGTTGTTC TTGCTTAACA 3301 ACATTGACCA AAAGATTGAC TCCATTTTCA ATGACCGCTA CATTATTTCG GTCTGCATCA 3361 GCTTCCTTGT GGTGTCGACG ATCACTGGTT TTTGTATTAT TCTTAAGGGC ATGTTGGACA 3421 AACGCTTAGC CATTGATTTT GTGAAAAATC AGTTTAAAAA CAAAAAGTTA TTCCGCAAGT 3481 GAAGAGCTTA GAGACC //