LOCUS       Exported     3587 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3587
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      1600..1647
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3475..3491
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      3492..3523
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0147"
                     /note="bac_cardiolipin: cardiolipin synthase"
                     /translation="MKKLIITVLSLIFMFGLTIFGLLLLNIYFFNWPLIGFGIFHLLAIIWAFIVLADKKRRFETRIRWFCFIVCIPIIGVLSYLFFGRSYKYKITKNYKFLEIQNKHSENELEQIDQILTNQIPQFKRAFKTASISQTNNIFLNTKVEFLENGNQLFLNLFKDIKNAKHYILLNFYIFKDGKLLDELTNLLIKKLKENVKVYIIYDFAGSYTLFEEAKIKLLEYGCQIVCYAPIRFPFIKWTANYRDHRKDIIIDNKIGYIGGINIGDEYINLDNKFGYWNDCSLKITGNAVSEIQRIFISDYDFYKPSFKKNSIKQDLDLDNVYSVKCKDQLVQIVSSGPNHEEPLHLSIFINLINSAQKRIWISTPYFIPPQEIRTALINAANSKLDVRILIPGLTDKAFLLDQTKQWTRELYKAGVKIYSINNVFNHNKTYLFDDEITFIGSTNLDFRALFADQQTMGLIYSKSLNKTISNKFEQDFKNSYLYDHLPNKNINWFRKIVIKIYNIIQPLL"
     primer_bind     complement(1652..1668)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1735..2323)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2494..3354)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3355..3459)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0147"
                     /note="bac_cardiolipin: cardiolipin synthase"
                     /translation="MKKLIITVLSLIFMFGLTIFGLLLLNIYFFNWPLIGFGIFHLLAIIWAFIVLADKKRRFETRIRWFCFIVCIPIIGVLSYLFFGRSYKYKITKNYKFLEIQNKHSENELEQIDQILTNQIPQFKRAFKTASISQTNNIFLNTKVEFLENGNQLFLNLFKDIKNAKHYILLNFYIFKDGKLLDELTNLLIKKLKENVKVYIIYDFAGSYTLFEEAKIKLLEYGCQIVCYAPIRFPFIKWTANYRDHRKDIIIDNKIGYIGGINIGDEYINLDNKFGYWNDCSLKITGNAVSEIQRIFISDYDFYKPSFKKNSIKQDLDLDNVYSVKCKDQLVQIVSSGPNHEEPLHLSIFINLINSAQKRIWISTPYFIPPQEIRTALINAANSKLDVRILIPGLTDKAFLLDQTKQWTRELYKAGVKIYSINNVFNHNKTYLFDDEITFIGSTNLDFRALFADQQTMGLIYSKSLNKTISNKFEQDFKNSYLYDHLPNKNINWFRKIVIKIYNIIQPLL"
ORIGIN
        1 ATGAAGAAGT TAATCATTAC CGTTCTGAGT CTGATTTTCA TGTTCGGATT GACGATTTTT
       61 GGCTTGTTGC TGTTGAACAT TTACTTCTTC AACTGGCCTC TGATCGGATT CGGGATTTTT
      121 CACTTATTGG CTATTATTTG GGCGTTCATT GTGTTGGCGG ACAAGAAGCG CCGCTTCGAG
      181 ACTCGCATCC GCTGGTTCTG TTTCATCGTA TGTATCCCTA TTATTGGGGT GCTTTCCTAT
      241 CTGTTTTTTG GACGTAGTTA TAAGTATAAG ATCACCAAAA ACTACAAGTT CTTAGAAATT
      301 CAGAACAAAC ACTCGGAAAA CGAGTTAGAG CAAATTGATC AGATCTTAAC AAACCAAATC
      361 CCTCAGTTTA AACGCGCTTT TAAGACAGCA AGCATCAGTC AAACCAATAA TATCTTTCTG
      421 AACACGAAGG TTGAGTTCCT TGAGAATGGC AATCAACTTT TTCTTAACCT TTTTAAGGAT
      481 ATTAAAAACG CAAAGCATTA TATCCTGTTG AATTTTTACA TTTTCAAAGA TGGGAAATTG
      541 CTGGATGAAT TGACAAACCT TTTAATCAAG AAATTGAAGG AGAACGTGAA AGTATACATC
      601 ATTTACGACT TTGCCGGTTC CTATACTCTT TTCGAAGAAG CGAAAATCAA ATTACTTGAA
      661 TATGGCTGCC AGATCGTGTG TTACGCCCCA ATCCGCTTTC CTTTTATTAA GTGGACGGCC
      721 AATTATCGTG ACCATCGTAA AGACATCATT ATTGACAACA AAATTGGCTA CATCGGTGGT
      781 ATCAACATTG GGGATGAATA CATCAATCTG GACAATAAAT TCGGATATTG GAATGATTGT
      841 AGTCTTAAAA TCACTGGAAA CGCAGTATCG GAAATTCAAC GTATCTTTAT TAGTGATTAC
      901 GATTTCTACA AGCCTTCGTT CAAAAAGAAC AGTATTAAGC AAGACCTTGA TTTAGACAAT
      961 GTATATTCCG TCAAGTGTAA GGATCAGTTA GTACAAATTG TGAGTTCCGG CCCTAACCAT
     1021 GAGGAACCCT TGCACTTAAG TATTTTTATC AACTTGATCA ATTCGGCTCA AAAACGTATC
     1081 TGGATCTCAA CGCCTTACTT CATCCCGCCC CAAGAGATTC GCACTGCATT AATTAATGCC
     1141 GCCAATTCGA AATTAGATGT ACGCATCCTT ATTCCCGGAT TGACAGATAA GGCATTTCTT
     1201 TTGGATCAAA CCAAGCAGTG GACTCGCGAA TTGTATAAAG CCGGTGTCAA AATCTACAGC
     1261 ATCAACAATG TTTTCAATCA CAACAAGACG TACTTGTTTG ATGACGAAAT CACCTTCATC
     1321 GGATCGACGA ACTTAGACTT CCGCGCCTTA TTCGCTGACC AGCAAACCAT GGGTTTAATT
     1381 TATTCGAAAT CTTTAAATAA AACAATCTCC AATAAATTTG AACAGGACTT TAAAAACTCC
     1441 TACTTGTACG ATCATCTGCC CAACAAAAAT ATCAACTGGT TTCGCAAGAT TGTCATCAAA
     1501 ATCTACAATA TTATCCAACC ATTGCTGTGA AGAGCTTAGA GACCGGTACT AGTTCCTGCA
     1561 GGGCTCGAGG TGTCAATCGT CGGAGCCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC
     1621 TCTAAACGGG TCTTGAGGGG TTTTTTGCAT GGTCATAGCT GTTTCCTGAG AGCTTGGCAG
     1681 GTGATGACAC ACATTAACAA ATTTCGTGAG GAGTCTCCAG AAGAATGCCA TTAATTTCCA
     1741 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA
     1801 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC
     1861 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC
     1921 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT
     1981 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG
     2041 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG
     2101 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA
     2161 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG
     2221 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT
     2281 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT
     2341 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG
     2401 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT
     2461 CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC
     2521 TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT
     2581 AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGAACC
     2641 ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG
     2701 AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG
     2761 AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT
     2821 GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG
     2881 AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT
     2941 TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC
     3001 TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC
     3061 ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA
     3121 TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG
     3181 AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC
     3241 CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG
     3301 GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT
     3361 CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT
     3421 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACCTGCACCA GTCAGTAAAA
     3481 CGACGGCCAG TAGTCAAAAG CCTCCGACCG GAGGCTTTTG ACTTGGTCAG GTGGAGTGAA
     3541 TTCGCGGCCG CTTCTAGAGG CTCTTCGTAA TTACGAAGAG GTCTCTA
//