LOCUS       Exported     4093 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4093
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             2010..4079
                     /note="Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome."
                     /translation="MAREYSLLNTRNIGIMAHIDAGKTTTTERILFHTGKIHKIGETHEGASQMDWMAQEQERGITITSAATTAFWKNTRFNIIDTPGHVDFTVEVERSLRVLDGAVAVLDGQSGVEPQTETVWRQATNYKVPRIVFVNKMDKTGADFIYSVKTIGDRLGAKAAPIQLPIGAEENFTGIIDLVEMKAYEFDGKPEENYKEIEIPTNLLEQAKELRAHLVEVAVEYDEELLMKFLDGGEISISELKSAIRKGVINADFFPVLAGSAFKNKGVKLLLDAVVDYLPSPLDIPSIKGILPTGEEVERHADDTEPFSALAFKVMTDPFVGKLTFFRVYSGILTKGSYVLNSTKQQKERVGRILQMHANNRTEIEEVYSGDIAAAVGLKNTTTGDTLCDEKGEIILESMVFPEPVIQLALEPKTKADQEKMSIALSKLAEEDPTFRTYTDDETGQTIIAGMGELHLDIIVDRMKREFNVATNVGAPQVSYRETIKLPGKAEGKYIKQSGGRGSYGHVVIEFEPNKDKGFEWVDKITGGRVSKEYINSARVGLENALRNGVIAGYPMIDVKATIVDGSMHEVDSNEMAYKIAASMALKEASKKMNPVVLEPIMNVEVTVPDEYYGDVMGNISSKRGIIEGSEQRGNAQTIKSKVPLTEMFGYATELRSFTQGRGNYTMIFSHYAEAPKAIADEIIKKSGK"
                     /label="MMSYN1_0150"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGGCGCGTGA GTACTCTTTG CTGAACACAC
     2041 GCAACATCGG AATTATGGCA CACATTGATG CTGGTAAGAC AACTACAACA GAGCGTATTC
     2101 TGTTTCATAC GGGGAAGATT CATAAGATTG GTGAAACACA CGAGGGGGCT TCCCAAATGG
     2161 ACTGGATGGC ACAAGAACAA GAGCGTGGCA TCACAATTAC TAGTGCTGCG ACGACTGCTT
     2221 TTTGGAAAAA TACACGTTTC AATATTATCG ACACACCAGG CCACGTGGAT TTTACTGTAG
     2281 AGGTTGAGCG TTCATTACGT GTGTTAGACG GAGCCGTTGC GGTCTTGGAC GGTCAATCTG
     2341 GTGTGGAACC CCAGACAGAA ACCGTGTGGC GTCAAGCTAC AAATTACAAA GTTCCTCGCA
     2401 TTGTTTTTGT CAACAAAATG GACAAAACAG GCGCGGATTT CATTTACTCC GTCAAAACGA
     2461 TTGGAGATCG TCTGGGGGCA AAAGCGGCTC CGATTCAATT ACCCATCGGG GCTGAGGAGA
     2521 ACTTCACAGG CATCATTGAT CTTGTCGAAA TGAAGGCGTA CGAATTTGAT GGAAAGCCTG
     2581 AGGAAAATTA TAAGGAGATT GAGATTCCTA CAAACCTTTT GGAACAGGCT AAGGAATTGC
     2641 GCGCACACCT TGTAGAAGTT GCTGTGGAAT ACGATGAAGA ACTTCTGATG AAATTTCTTG
     2701 ATGGTGGGGA AATTTCCATC TCAGAGCTTA AGTCAGCAAT CCGTAAAGGT GTCATCAACG
     2761 CGGATTTCTT CCCCGTATTG GCCGGATCCG CCTTTAAGAA CAAAGGCGTA AAGTTGCTTT
     2821 TGGACGCGGT GGTAGATTAT TTACCATCCC CCTTAGACAT TCCCTCTATT AAAGGTATTT
     2881 TACCAACTGG GGAGGAAGTA GAGCGCCACG CGGACGATAC TGAGCCGTTT TCTGCATTGG
     2941 CCTTTAAGGT AATGACAGAC CCATTTGTAG GAAAGTTAAC CTTCTTCCGC GTGTATTCCG
     3001 GTATCCTGAC TAAGGGCTCG TATGTGTTGA ACTCTACTAA GCAACAGAAG GAGCGTGTCG
     3061 GTCGCATTTT GCAGATGCAT GCGAATAATC GTACGGAAAT CGAAGAGGTG TATAGCGGCG
     3121 ACATTGCCGC AGCCGTAGGC TTGAAAAATA CCACTACGGG GGATACCTTA TGCGACGAAA
     3181 AGGGCGAAAT TATCCTTGAA TCTATGGTTT TCCCAGAGCC AGTGATCCAA TTGGCGTTAG
     3241 AGCCCAAGAC GAAGGCCGAC CAAGAGAAAA TGAGTATCGC GTTATCAAAA CTGGCTGAGG
     3301 AGGATCCGAC ATTCCGTACA TACACAGACG ATGAAACGGG CCAGACAATT ATCGCTGGTA
     3361 TGGGGGAACT TCATTTAGAT ATCATTGTCG ACCGTATGAA GCGTGAATTC AACGTAGCTA
     3421 CGAACGTAGG GGCGCCACAG GTTAGTTACC GCGAAACCAT CAAGTTGCCG GGCAAGGCCG
     3481 AGGGCAAATA TATCAAACAA TCTGGAGGTC GCGGCTCCTA CGGGCACGTG GTTATCGAAT
     3541 TCGAGCCGAA TAAAGATAAG GGCTTTGAGT GGGTTGATAA GATTACGGGC GGACGCGTCA
     3601 GTAAGGAGTA TATCAACAGT GCCCGTGTAG GATTGGAAAA CGCCTTGCGC AACGGGGTTA
     3661 TCGCTGGGTA CCCGATGATC GATGTTAAGG CAACGATTGT GGACGGTTCC ATGCATGAAG
     3721 TAGATTCAAA CGAAATGGCG TACAAAATCG CGGCATCTAT GGCTCTTAAG GAAGCCTCCA
     3781 AGAAAATGAA CCCTGTGGTG CTGGAGCCCA TCATGAACGT TGAGGTCACG GTCCCTGATG
     3841 AGTACTACGG TGACGTAATG GGGAACATCT CTTCTAAACG CGGCATCATT GAAGGAAGCG
     3901 AACAGCGCGG TAATGCACAG ACCATCAAAA GTAAAGTGCC CTTAACGGAA ATGTTCGGTT
     3961 ATGCCACTGA GCTGCGCTCC TTCACGCAGG GACGTGGGAA CTACACTATG ATCTTCTCTC
     4021 ACTACGCTGA AGCCCCAAAG GCAATCGCTG ATGAAATCAT TAAGAAATCA GGAAAGTGAA
     4081 GAGCTTAGAG ACC
//