LOCUS       Exported     3379 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3379
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      1416..1463
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3311..3327
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3328..3359
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             9..1364
                     /label="MMSYN1_0154"
                     /note=""
                     /translation="MIKFNDKKEELTLVCLTEVNNKPYVSDADLSTTFISEDKTIYMVIKKDHKCLKTKIRNAFKKFVSTNKFNINVDVDSFLVFFDMCGCKKDAIEGIYESIAFETFDKVSYKKDTKPNEVVYNLITSEDVKELEQKEAIKMEFVNFARMLQDTPPNIATSEYLAEKVVQKAKEIEGLKVTVLNKKEATKLGMNLFLAVNAGSPYEPQAVVLEYVGDENEPKKALVGKGITFDSGGYNLKPSSAMEGMKFDMSGAAIMLSTVMALAKMKAKVNVVGIGMFTDNRIGSTATLPQSVIKSMNGLTVEIDNTDAEGRLVLADGITYVIREKQATEIWEASTLTGAMVIALGSFATGVFTNCDKRWELISQASHKTSEKVWRMPIFDEHLEKVKADSVNADLTNAAKGREAGSSTAAAFLSAFAEEKPFVHFDIAGTADKAGRGQGVLVRTLFEIFNK"
     primer_bind     complement(1468..1484)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1551..2139)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2330..3190)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3191..3295)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 GGTCTCAAAT GATTAAATTT AACGACAAAA AGGAAGAGTT GACTCTGGTC TGTCTTACTG
       61 AAGTTAACAA TAAGCCATAC GTTAGTGATG CTGACTTGTC TACTACCTTT ATTTCGGAGG
      121 ACAAAACGAT TTACATGGTG ATCAAGAAGG ATCATAAATG TTTAAAGACT AAGATTCGTA
      181 ACGCTTTCAA GAAGTTTGTC TCCACCAACA AATTTAATAT CAATGTTGAT GTTGACAGTT
      241 TTCTTGTCTT TTTCGACATG TGTGGTTGTA AGAAAGATGC AATCGAGGGA ATCTACGAGT
      301 CGATTGCTTT TGAAACCTTT GACAAGGTGA GTTATAAAAA GGATACTAAA CCAAATGAGG
      361 TCGTCTACAA CCTTATCACA AGCGAGGATG TCAAGGAGCT TGAACAGAAA GAAGCAATCA
      421 AAATGGAATT TGTGAACTTC GCACGCATGT TACAGGACAC ACCGCCAAAT ATTGCAACTA
      481 GCGAATATCT TGCAGAAAAG GTGGTGCAAA AAGCGAAGGA AATTGAAGGA TTGAAAGTCA
      541 CAGTTCTTAA CAAGAAGGAA GCTACAAAGT TGGGAATGAA TCTGTTTTTA GCGGTTAACG
      601 CAGGATCACC TTACGAACCG CAGGCCGTAG TACTGGAATA CGTCGGTGAC GAGAACGAAC
      661 CCAAGAAAGC CTTAGTAGGT AAGGGCATTA CATTTGATTC CGGGGGCTAT AACTTGAAAC
      721 CTAGTTCAGC TATGGAAGGT ATGAAATTTG ATATGAGCGG TGCGGCTATT ATGCTGAGCA
      781 CCGTGATGGC GCTTGCCAAA ATGAAGGCCA AGGTGAACGT AGTCGGTATT GGAATGTTCA
      841 CGGATAATCG TATCGGATCA ACTGCAACCC TTCCGCAGAG TGTGATTAAA TCAATGAATG
      901 GTCTGACAGT AGAGATTGAT AACACTGACG CAGAGGGTCG CTTGGTCTTG GCGGATGGTA
      961 TTACGTACGT AATTCGTGAG AAGCAAGCAA CAGAGATCTG GGAAGCCAGT ACTTTAACTG
     1021 GTGCTATGGT AATCGCGTTG GGCTCCTTCG CAACCGGTGT CTTTACAAAT TGCGATAAAC
     1081 GCTGGGAGCT GATTTCTCAG GCCTCTCATA AAACATCCGA GAAAGTGTGG CGTATGCCTA
     1141 TCTTCGACGA GCACTTGGAG AAAGTCAAAG CTGATTCGGT TAACGCTGAT CTTACAAATG
     1201 CGGCGAAAGG TCGTGAGGCC GGAAGTTCAA CGGCTGCTGC GTTTTTAAGT GCTTTTGCCG
     1261 AAGAAAAGCC TTTCGTCCAC TTCGATATTG CAGGAACAGC CGATAAGGCC GGTCGCGGTC
     1321 AGGGGGTTCT GGTGCGTACT CTTTTTGAGA TTTTCAATAA GTGAAGAGCT TAGAGACCCG
     1381 CTGAGGTGTC AATCGTCGGA GCCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA
     1441 AACGGGTCTT GAGGGGTTTT TTGCATGGTC ATAGCTGTTT CCTGAGAGCT TGGCAGGTGA
     1501 TGACACACAT TAACAAATTT CGTGAGGAGT CTCCAGAAGA ATGCCATTAA TTTCCATAGG
     1561 CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG
     1621 ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT
     1681 CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT
     1741 TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC
     1801 TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT
     1861 GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT
     1921 AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC
     1981 TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA
     2041 AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT
     2101 TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGGCCTACT ATTAGCAACA
     2161 ACGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG
     2221 GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT
     2281 GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT
     2341 TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC
     2401 TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA
     2461 TGATACCGCG AGAACCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG
     2521 GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT
     2581 GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA
     2641 TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT
     2701 CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT
     2761 TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG
     2821 CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG
     2881 AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG
     2941 CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA
     3001 AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT
     3061 AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT
     3121 GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT
     3181 GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA
     3241 TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACCT
     3301 GCACCAGTCA GTAAAACGAC GGCCAGTAGT CAAAAGCCTC CGACCGGAGG CTTTTGACTT
     3361 GGTTCAGGTG GAGTGGGAG
//