LOCUS       Exported     4714 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4714
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2010..4700
                     /label="MMSYN1_0163"
                     /note="AlaRS: Alanyl-tRNA synthetase"
                     /translation="MKKLSTNQIRKIWLDFFISKNHYFLETVSLIPVDDPSLLWINSGVATLKPYFDGRKTPPSPRLTNSQKAIRTNDIENVGVTARHHTMFEMLGNFSIGDYFKKEAIELAWELLTDKNYFDIDKDKLYITVFNEDTEAYNIWKDVIKIDEDHIFRLSRKTNFWDVGQGPCGPNTEIFYDRGEIWDPNKIGPRLISDDIENDRYIEVWNIVFSQFNNDGNDNYTELPRKNIDTGAGLERFASIFQNTPTNFETDIFYPTIKKVEQLTNDQFKYSIDNYFNPNKKQTRINTAFKVIADHIRATVFAISDGVFPGNKDRGYIIRRLIRRSCVFGKELGIKQAFLYKLVDSVIESMKEFYPYLIDKKTLVEQTIKDEEEKFLKTLSKGYDLLENIIKTKNTVSDKDALLLFESYGFPIEQTIEISELSNVTVDIEGFKKLLEQTKQATRNARKDLKAWDKQNELFTKLKVESEFTGWSETSRDNAKVIYMFTDQKQVESITDQEAFVILDKTPFYAEKGGQAADSGIIFNDQMKGFVIDVQQGPMHQNIHRIKVQGTLKVNDLVNCRVDEEKRIYTMKNHSGTHMIHYALREVLGTSVMQSGSYNDENGLRMDFTYHRLPTNQELLKAQNLVLEKIKNKIDRQTYFCSLEESVKKHQALAFFTEKYDEIVRVIKFGDFSSELCGGTHVNNTSEIEDFIITGIESKGSGLYRIKCLTSFKTVNEYLNEQFKIYKDQAEIIIDKYNQNKDLLKNDQLENIYLQIKNITINKDNLLVIKDLLDKLKEINKDYDKKVNDLITANKLLKYKDLTPSLNKDNINEIRLETTDLNIKDLKQLADDLRNKYDDLIVILLSSTSENNFVVVAVSQSLQNKYKAIDIFNNLEGYETKGGGNANLAQGKFVKK"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGAAGAAACT TTCAACGAAC CAAATTCGTA
     2041 AGATCTGGCT TGACTTTTTT ATCTCAAAAA ATCACTACTT CTTAGAGACA GTCAGCTTGA
     2101 TCCCTGTCGA TGACCCCTCT CTTCTTTGGA TTAACTCTGG AGTTGCTACG CTGAAGCCTT
     2161 ACTTCGATGG GCGCAAAACA CCACCTTCCC CACGTCTTAC GAATTCGCAA AAAGCCATCC
     2221 GTACGAATGA TATCGAGAAT GTGGGCGTCA CGGCACGTCA CCATACCATG TTCGAGATGC
     2281 TTGGGAACTT CTCTATCGGC GATTACTTTA AGAAGGAGGC GATCGAATTG GCCTGGGAGT
     2341 TACTTACTGA CAAAAATTAT TTTGATATCG ATAAAGACAA GCTTTACATT ACAGTGTTTA
     2401 ACGAAGATAC AGAGGCCTAC AATATCTGGA AAGACGTAAT CAAGATTGAT GAGGATCATA
     2461 TCTTTCGTCT GAGCCGTAAA ACAAACTTTT GGGACGTGGG GCAAGGGCCA TGTGGGCCGA
     2521 ATACGGAAAT CTTTTACGAT CGTGGCGAGA TTTGGGATCC GAATAAAATC GGCCCACGCC
     2581 TGATTTCAGA CGACATCGAA AACGATCGTT ATATCGAAGT TTGGAACATC GTTTTTAGTC
     2641 AGTTTAATAA CGACGGGAAC GATAACTATA CGGAGCTGCC ACGTAAGAAC ATTGACACTG
     2701 GCGCCGGTCT GGAGCGTTTT GCGTCCATTT TTCAGAATAC CCCGACTAAC TTTGAAACAG
     2761 ATATTTTCTA TCCAACTATC AAAAAGGTCG AACAATTGAC TAACGACCAG TTCAAGTACT
     2821 CTATTGATAA TTACTTTAAT CCCAATAAAA AACAAACCCG CATTAATACT GCGTTTAAAG
     2881 TTATCGCCGA TCATATCCGT GCCACAGTTT TCGCAATCTC GGACGGCGTT TTTCCTGGTA
     2941 ATAAGGACCG CGGTTATATT ATTCGCCGCC TGATCCGCCG TTCATGCGTA TTCGGTAAAG
     3001 AATTAGGGAT TAAGCAAGCC TTCCTGTACA AGTTAGTAGA TAGCGTCATC GAGTCGATGA
     3061 AAGAATTCTA CCCGTACTTG ATCGACAAGA AAACACTTGT TGAGCAGACA ATCAAGGACG
     3121 AGGAGGAAAA ATTCCTTAAG ACCTTATCCA AAGGGTATGA TTTGCTGGAA AACATTATCA
     3181 AGACTAAGAA CACTGTAAGT GACAAAGACG CATTACTGTT ATTTGAGTCC TATGGATTTC
     3241 CCATTGAACA AACAATCGAA ATCAGTGAGC TGAGTAACGT GACAGTGGAC ATTGAGGGCT
     3301 TTAAAAAACT TCTGGAGCAA ACAAAGCAGG CCACACGTAA TGCGCGTAAA GACTTAAAGG
     3361 CCTGGGACAA ACAAAACGAG CTGTTCACGA AGTTGAAGGT TGAATCAGAA TTCACTGGGT
     3421 GGTCAGAAAC TTCACGCGAC AATGCGAAAG TGATCTACAT GTTCACGGAT CAAAAACAGG
     3481 TAGAGTCAAT TACCGACCAG GAAGCATTCG TGATCTTAGA TAAAACACCC TTTTACGCCG
     3541 AAAAAGGCGG TCAGGCGGCA GACTCTGGGA TCATCTTTAA TGATCAGATG AAGGGTTTCG
     3601 TAATCGACGT TCAGCAGGGT CCAATGCACC AAAACATCCA CCGCATCAAG GTGCAGGGGA
     3661 CCTTGAAGGT AAATGACCTG GTCAACTGTC GTGTGGATGA AGAGAAGCGC ATCTATACTA
     3721 TGAAGAATCA CTCGGGGACA CATATGATTC ACTACGCATT ACGTGAAGTA CTGGGTACGT
     3781 CGGTAATGCA GTCCGGTAGC TACAACGATG AAAATGGGTT ACGCATGGAC TTCACTTATC
     3841 ACCGTCTGCC CACCAACCAA GAGTTACTGA AGGCCCAGAA TTTAGTATTG GAGAAAATCA
     3901 AGAACAAAAT TGACCGTCAG ACCTATTTCT GTTCGTTAGA AGAGTCAGTC AAAAAGCACC
     3961 AAGCCCTGGC GTTCTTTACT GAGAAGTATG ATGAGATCGT ACGCGTCATC AAATTTGGTG
     4021 ACTTTTCATC TGAGTTATGT GGCGGTACAC ATGTGAATAA CACATCGGAA ATTGAGGACT
     4081 TTATTATTAC AGGCATCGAG TCAAAGGGGT CTGGATTATA TCGTATTAAA TGCTTAACTA
     4141 GCTTTAAGAC AGTAAACGAG TACCTGAACG AGCAATTCAA AATTTACAAA GACCAAGCGG
     4201 AGATCATTAT TGATAAGTAC AACCAGAACA AAGACTTGTT GAAGAATGAC CAGCTTGAGA
     4261 ATATCTATTT GCAGATTAAG AACATTACGA TTAATAAGGA TAATTTGTTA GTTATCAAAG
     4321 ACTTATTAGA CAAACTTAAA GAAATCAACA AGGACTACGA CAAAAAGGTG AACGACCTTA
     4381 TTACGGCGAA TAAATTGCTT AAATACAAAG ATTTAACGCC AAGCTTAAAT AAGGACAACA
     4441 TTAATGAGAT CCGCTTAGAA ACCACCGACT TGAATATTAA GGATTTAAAA CAGTTAGCCG
     4501 ATGATCTGCG TAACAAGTAT GACGACCTGA TTGTTATCTT GCTTTCATCT ACGAGTGAGA
     4561 ATAATTTTGT GGTCGTTGCG GTCAGTCAAA GCTTACAGAA CAAATATAAA GCGATCGACA
     4621 TTTTCAATAA CCTTGAAGGC TACGAAACTA AAGGAGGGGG GAATGCCAAT CTGGCGCAAG
     4681 GTAAGTTTGT AAAGAAGTGA AGAGCTTAGA GACC
//