LOCUS       Exported     3302 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3302
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1315..1362
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3190..3206
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3207..3238
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0165"
                     /note=""
                     /translation="MKSTLKTKQEVLNLNSELLLDDFSLLNETNQQHKVSKWTTFKYWYYDTSANIYKYFLRHPLYGYSFKRILYGLITLLLSIIILYVVIRLITPDTKYLPPDIEKTGLSRAQQDKLLEDRMKRFGVYGPLIPQILTYLKNITPFIPKQIVLGSEVTILQNGNAIIDSSKLITETRWVYLGVTTATTIAEEGSDALSIFLKAMPYSFAIGSVSVLISYALAILIGVRAAKKKGKLFDNVFNGISALLLAIPSIVIIIGTFIFSVAVLGNSGIYNTGSFATRFWPIFAIVVINLPGIATFVRRYIVDEMTVDYAKFALAKGTSSNKTYYVHIFRNAGVRIIRSIPSEIILTVFGSSMIVETQWAIPGMGRLIKESAGGNDFFVFLGFTVLSSFVSIFAKLLADLVHVLLDPRVSLTKD"
     primer_bind     complement(1367..1383)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(1450..2038)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2209..3069)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3070..3174)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0165"
                     /note=""
                     /translation="MKSTLKTKQEVLNLNSELLLDDFSLLNETNQQHKVSKWTTFKYWYYDTSANIYKYFLRHPLYGYSFKRILYGLITLLLSIIILYVVIRLITPDTKYLPPDIEKTGLSRAQQDKLLEDRMKRFGVYGPLIPQILTYLKNITPFIPKQIVLGSEVTILQNGNAIIDSSKLITETRWVYLGVTTATTIAEEGSDALSIFLKAMPYSFAIGSVSVLISYALAILIGVRAAKKKGKLFDNVFNGISALLLAIPSIVIIIGTFIFSVAVLGNSGIYNTGSFATRFWPIFAIVVINLPGIATFVRRYIVDEMTVDYAKFALAKGTSSNKTYYVHIFRNAGVRIIRSIPSEIILTVFGSSMIVETQWAIPGMGRLIKESAGGNDFFVFLGFTVLSSFVSIFAKLLADLVHVLLDPRVSLTKD"
ORIGIN
        1 ATGAAATCAA CATTGAAAAC AAAACAGGAG GTTCTGAACT TGAATTCAGA ACTTCTGTTG
       61 GACGACTTCA GCCTTTTAAA CGAAACAAAT CAACAGCACA AGGTGTCTAA ATGGACTACC
      121 TTCAAATACT GGTATTATGA TACTAGCGCA AATATCTATA AATATTTCCT TCGCCATCCT
      181 CTGTACGGTT ATAGCTTTAA GCGCATCTTG TATGGTTTAA TCACACTTCT GTTGTCTATC
      241 ATCATCCTGT ATGTGGTGAT CCGTTTGATC ACACCCGACA CGAAATATCT TCCACCCGAT
      301 ATCGAAAAGA CGGGTCTGTC ACGCGCGCAG CAGGATAAGT TATTGGAAGA TCGTATGAAG
      361 CGCTTCGGAG TGTACGGTCC TCTTATCCCC CAAATCCTTA CTTACTTGAA GAATATCACC
      421 CCATTCATTC CTAAACAAAT TGTCTTGGGA AGTGAGGTTA CAATTCTTCA AAATGGAAAC
      481 GCAATCATCG ATTCATCCAA ACTTATCACC GAGACTCGTT GGGTTTATTT AGGCGTGACA
      541 ACAGCTACGA CGATTGCGGA AGAGGGTTCC GACGCTCTGT CCATTTTTCT GAAAGCTATG
      601 CCATATAGCT TCGCTATCGG GTCAGTCTCT GTTTTAATCT CCTATGCTTT AGCGATCTTA
      661 ATCGGCGTCC GTGCGGCAAA GAAGAAGGGT AAACTGTTTG ACAACGTGTT CAATGGTATT
      721 AGCGCATTAT TGTTGGCCAT TCCGTCCATC GTAATCATTA TTGGAACGTT CATTTTTTCG
      781 GTGGCTGTAT TAGGTAACAG CGGGATCTAT AATACTGGGA GTTTTGCGAC ACGTTTTTGG
      841 CCAATTTTCG CGATTGTCGT AATCAACTTG CCCGGTATTG CGACCTTTGT ACGCCGCTAT
      901 ATTGTTGATG AGATGACCGT GGACTATGCC AAGTTCGCCT TGGCAAAGGG CACATCTTCT
      961 AACAAAACGT ATTACGTTCA CATCTTTCGC AACGCTGGTG TCCGTATTAT TCGCTCGATT
     1021 CCAAGCGAGA TCATCCTTAC AGTATTCGGT AGCTCCATGA TCGTAGAAAC ACAGTGGGCG
     1081 ATCCCTGGTA TGGGTCGTTT GATCAAGGAG AGTGCGGGTG GTAACGATTT TTTCGTTTTC
     1141 TTAGGGTTTA CAGTGCTGTC AAGTTTTGTC TCGATCTTTG CGAAGCTGTT GGCTGACCTG
     1201 GTTCATGTCC TTCTTGATCC TCGTGTCAGT TTGACGAAGG ATTGAAGAGC TTAGAGACCG
     1261 GTACTAGTTC CTGCAGGGCT CGAGGTGTCA ATCGTCGGAG CCGCTGAGCA ATAACTAGCA
     1321 TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCATGGTCA TAGCTGTTTC
     1381 CTGAGAGCTT GGCAGGTGAT GACACACATT AACAAATTTC GTGAGGAGTC TCCAGAAGAA
     1441 TGCCATTAAT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG
     1501 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC
     1561 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC
     1621 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT
     1681 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT
     1741 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC
     1801 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA
     1861 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA
     1921 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG
     1981 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA
     2041 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG
     2101 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG
     2161 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT
     2221 AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT
     2281 CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT
     2341 GATACCGCGA GAACCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG
     2401 AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG
     2461 TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT
     2521 TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC
     2581 CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT
     2641 CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC
     2701 AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA
     2761 GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC
     2821 GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA
     2881 ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA
     2941 ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG
     3001 AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG
     3061 AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT
     3121 GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACCTG
     3181 CACCAGTCAG TAAAACGACG GCCAGTAGTC AAAAGCCTCC GACCGGAGGC TTTTGACTTG
     3241 GTCAGGTGGA GTGAATTCGC GGCCGCTTCT AGAGGCTCTT CGTAATTACG AAGAGGTCTC
     3301 TA
//