LOCUS       Exported     3892 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3892
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
                     /label="T7 terminator"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2010..3878
                     /label="MMSYN1_0168"
                     /note="Putative AmiF"
                     /translation="MIKKKNEAILKVRDLLIEFGNGRNKLKAVKGVTFDVYKGETFGLVGESGSGKTTIGRAIIGIQPISDGAIYFENKLLRGKSPDVYKINQKIARHLYIMQQNQLTTSLSLNDYSNEFKRVYYKYVQSKFFDFKTQELKDYEDGKSRIIKEGVNLNTTKLVSVKKNANLSIVIQAITDNLKRLLKIIRLQEKASRITKNISKHTSVKVELQDAINKYQDFVHDSILKVKDLENTIYNTLQEMLAIRNDVNEGKYTSVTKFFDQMGSRLKLVIKSQKLITPQLEDASHDQLMNLALTCPKYKNNYYLKKLKQRIEYLNLNNKTKLAQEYESVIQTVENSDFYDNLKTAEIFKSPNKKELKENKKDMQMIFQDPSSSLNERMAVEEIIKEGLDNFPELYSNDEVKKAYQQWFNQKNPENKIVEISEIDKKDIKRFLINQLLETVGLLPEHLSRYPHEFSGGQRQRIGIARALIMKPKFVVADEPISALDVSIRAQIMNLLAKFQKQFDLTYIFIAHDLSVVRFATDRIAVIYRGDIVELAESNELFDLPLHPYTRSLLSAIPLPDPVQESKKVHFVYQPEVEHHDYLVDFPKWVEVSKNHFVYANEREIKAYKKQIKAYKEQLKNK"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGATTAAGAA AAAGAATGAA GCTATTCTGA
     2041 AGGTACGTGA CTTGTTGATT GAATTTGGAA ATGGCCGCAA TAAACTGAAA GCCGTCAAGG
     2101 GTGTGACGTT TGACGTTTAT AAGGGTGAAA CGTTTGGTCT TGTTGGAGAG AGTGGCTCTG
     2161 GCAAAACAAC GATCGGACGT GCAATCATTG GCATTCAGCC TATCAGTGAC GGTGCGATTT
     2221 ACTTTGAGAA CAAATTGTTG CGCGGAAAGA GTCCCGATGT ATATAAAATT AATCAGAAAA
     2281 TCGCCCGTCA CCTGTATATT ATGCAGCAAA ACCAGTTGAC CACGAGCTTA AGTCTGAATG
     2341 ACTATTCCAA CGAGTTTAAG CGCGTATACT ATAAGTATGT ACAAAGCAAA TTCTTTGACT
     2401 TCAAGACCCA AGAGTTAAAA GATTACGAGG ACGGGAAGTC ACGCATTATT AAGGAGGGAG
     2461 TTAATCTTAA TACTACTAAA TTGGTTAGCG TCAAGAAAAA CGCCAACTTA TCCATCGTCA
     2521 TTCAAGCAAT TACAGATAAT TTGAAGCGTT TGTTGAAAAT CATCCGCCTT CAAGAAAAGG
     2581 CCAGTCGTAT TACAAAAAAC ATTAGCAAAC ACACGAGCGT TAAGGTTGAA TTGCAAGATG
     2641 CAATTAACAA GTATCAAGAT TTCGTCCACG ATTCCATCTT GAAAGTTAAA GATTTGGAGA
     2701 ATACGATCTA TAACACCTTG CAAGAGATGT TAGCCATCCG CAACGACGTT AACGAGGGGA
     2761 AGTACACATC GGTCACAAAG TTTTTTGATC AGATGGGCTC TCGCTTAAAG TTGGTCATTA
     2821 AATCTCAAAA ATTAATTACA CCACAGTTAG AGGACGCTTC TCACGATCAA TTAATGAACT
     2881 TAGCCTTGAC TTGTCCTAAA TATAAGAATA ATTACTACTT GAAGAAATTG AAACAACGTA
     2941 TCGAATATTT GAACTTAAAT AACAAGACCA AGTTGGCGCA GGAGTACGAA TCGGTCATCC
     3001 AAACTGTCGA AAACTCAGAT TTCTATGACA ATCTGAAAAC CGCCGAAATT TTCAAGTCGC
     3061 CAAACAAAAA GGAATTAAAG GAAAATAAGA AAGACATGCA AATGATTTTC CAAGATCCCT
     3121 CGTCGTCGCT GAACGAGCGC ATGGCGGTAG AGGAAATCAT TAAAGAGGGT CTTGATAACT
     3181 TCCCCGAGTT ATACAGCAAC GATGAAGTGA AAAAGGCATA CCAGCAGTGG TTTAACCAGA
     3241 AAAATCCTGA GAACAAGATC GTTGAGATTT CCGAGATTGA TAAAAAAGAT ATCAAACGCT
     3301 TTCTTATCAA TCAACTGTTG GAAACGGTGG GACTGTTACC GGAACACTTG AGCCGCTACC
     3361 CGCACGAGTT TTCGGGAGGT CAACGTCAAC GTATCGGGAT CGCTCGCGCG TTAATTATGA
     3421 AACCCAAATT TGTTGTCGCC GATGAGCCGA TCTCGGCATT GGATGTTTCC ATTCGTGCTC
     3481 AAATCATGAA TCTGCTTGCC AAATTTCAAA AGCAGTTCGA TTTAACCTAC ATTTTTATCG
     3541 CGCATGACTT AAGCGTCGTT CGCTTCGCCA CCGACCGTAT TGCCGTTATC TACCGCGGAG
     3601 ATATCGTGGA GTTAGCCGAG TCAAACGAAT TGTTCGATCT GCCCTTACAT CCGTATACGC
     3661 GTTCGCTTTT GTCTGCGATC CCTCTTCCGG ACCCTGTGCA GGAATCGAAA AAGGTACACT
     3721 TTGTATATCA ACCCGAGGTA GAACATCATG ATTATCTGGT AGATTTTCCG AAATGGGTTG
     3781 AGGTGAGTAA AAACCATTTT GTCTACGCAA ACGAACGCGA GATTAAGGCG TATAAGAAAC
     3841 AGATTAAGGC TTACAAAGAA CAGCTGAAAA ACAAGTGAAG AGCTTAGAGA CC
//