LOCUS Exported 3892 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3892 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 38..85 /note="transcription terminator for bacteriophage T7 RNA polymerase" /label="T7 terminator" primer_bind 1933..1949 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 1950..1981 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 2010..3878 /label="MMSYN1_0168" /note="Putative AmiF" /translation="MIKKKNEAILKVRDLLIEFGNGRNKLKAVKGVTFDVYKGETFGLVGESGSGKTTIGRAIIGIQPISDGAIYFENKLLRGKSPDVYKINQKIARHLYIMQQNQLTTSLSLNDYSNEFKRVYYKYVQSKFFDFKTQELKDYEDGKSRIIKEGVNLNTTKLVSVKKNANLSIVIQAITDNLKRLLKIIRLQEKASRITKNISKHTSVKVELQDAINKYQDFVHDSILKVKDLENTIYNTLQEMLAIRNDVNEGKYTSVTKFFDQMGSRLKLVIKSQKLITPQLEDASHDQLMNLALTCPKYKNNYYLKKLKQRIEYLNLNNKTKLAQEYESVIQTVENSDFYDNLKTAEIFKSPNKKELKENKKDMQMIFQDPSSSLNERMAVEEIIKEGLDNFPELYSNDEVKKAYQQWFNQKNPENKIVEISEIDKKDIKRFLINQLLETVGLLPEHLSRYPHEFSGGQRQRIGIARALIMKPKFVVADEPISALDVSIRAQIMNLLAKFQKQFDLTYIFIAHDLSVVRFATDRIAVIYRGDIVELAESNELFDLPLHPYTRSLLSAIPLPDPVQESKKVHFVYQPEVEHHDYLVDFPKWVEVSKNHFVYANEREIKAYKKQIKAYKEQLKNK" primer_bind complement(90..106) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(173..761) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(952..1812) /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" /label="bla" promoter complement(1813..1917) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC 61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT 121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA 181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG 721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA 781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG 961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG 1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC 1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC 1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA 1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC 1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG 1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC 1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT 1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG 1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC 1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG 1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT 1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG 1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG 1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC 1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGATTAAGAA AAAGAATGAA GCTATTCTGA 2041 AGGTACGTGA CTTGTTGATT GAATTTGGAA ATGGCCGCAA TAAACTGAAA GCCGTCAAGG 2101 GTGTGACGTT TGACGTTTAT AAGGGTGAAA CGTTTGGTCT TGTTGGAGAG AGTGGCTCTG 2161 GCAAAACAAC GATCGGACGT GCAATCATTG GCATTCAGCC TATCAGTGAC GGTGCGATTT 2221 ACTTTGAGAA CAAATTGTTG CGCGGAAAGA GTCCCGATGT ATATAAAATT AATCAGAAAA 2281 TCGCCCGTCA CCTGTATATT ATGCAGCAAA ACCAGTTGAC CACGAGCTTA AGTCTGAATG 2341 ACTATTCCAA CGAGTTTAAG CGCGTATACT ATAAGTATGT ACAAAGCAAA TTCTTTGACT 2401 TCAAGACCCA AGAGTTAAAA GATTACGAGG ACGGGAAGTC ACGCATTATT AAGGAGGGAG 2461 TTAATCTTAA TACTACTAAA TTGGTTAGCG TCAAGAAAAA CGCCAACTTA TCCATCGTCA 2521 TTCAAGCAAT TACAGATAAT TTGAAGCGTT TGTTGAAAAT CATCCGCCTT CAAGAAAAGG 2581 CCAGTCGTAT TACAAAAAAC ATTAGCAAAC ACACGAGCGT TAAGGTTGAA TTGCAAGATG 2641 CAATTAACAA GTATCAAGAT TTCGTCCACG ATTCCATCTT GAAAGTTAAA GATTTGGAGA 2701 ATACGATCTA TAACACCTTG CAAGAGATGT TAGCCATCCG CAACGACGTT AACGAGGGGA 2761 AGTACACATC GGTCACAAAG TTTTTTGATC AGATGGGCTC TCGCTTAAAG TTGGTCATTA 2821 AATCTCAAAA ATTAATTACA CCACAGTTAG AGGACGCTTC TCACGATCAA TTAATGAACT 2881 TAGCCTTGAC TTGTCCTAAA TATAAGAATA ATTACTACTT GAAGAAATTG AAACAACGTA 2941 TCGAATATTT GAACTTAAAT AACAAGACCA AGTTGGCGCA GGAGTACGAA TCGGTCATCC 3001 AAACTGTCGA AAACTCAGAT TTCTATGACA ATCTGAAAAC CGCCGAAATT TTCAAGTCGC 3061 CAAACAAAAA GGAATTAAAG GAAAATAAGA AAGACATGCA AATGATTTTC CAAGATCCCT 3121 CGTCGTCGCT GAACGAGCGC ATGGCGGTAG AGGAAATCAT TAAAGAGGGT CTTGATAACT 3181 TCCCCGAGTT ATACAGCAAC GATGAAGTGA AAAAGGCATA CCAGCAGTGG TTTAACCAGA 3241 AAAATCCTGA GAACAAGATC GTTGAGATTT CCGAGATTGA TAAAAAAGAT ATCAAACGCT 3301 TTCTTATCAA TCAACTGTTG GAAACGGTGG GACTGTTACC GGAACACTTG AGCCGCTACC 3361 CGCACGAGTT TTCGGGAGGT CAACGTCAAC GTATCGGGAT CGCTCGCGCG TTAATTATGA 3421 AACCCAAATT TGTTGTCGCC GATGAGCCGA TCTCGGCATT GGATGTTTCC ATTCGTGCTC 3481 AAATCATGAA TCTGCTTGCC AAATTTCAAA AGCAGTTCGA TTTAACCTAC ATTTTTATCG 3541 CGCATGACTT AAGCGTCGTT CGCTTCGCCA CCGACCGTAT TGCCGTTATC TACCGCGGAG 3601 ATATCGTGGA GTTAGCCGAG TCAAACGAAT TGTTCGATCT GCCCTTACAT CCGTATACGC 3661 GTTCGCTTTT GTCTGCGATC CCTCTTCCGG ACCCTGTGCA GGAATCGAAA AAGGTACACT 3721 TTGTATATCA ACCCGAGGTA GAACATCATG ATTATCTGGT AGATTTTCCG AAATGGGTTG 3781 AGGTGAGTAA AAACCATTTT GTCTACGCAA ACGAACGCGA GATTAAGGCG TATAAGAAAC 3841 AGATTAAGGC TTACAAAGAA CAGCTGAAAA ACAAGTGAAG AGCTTAGAGA CC //