LOCUS       Exported     2615 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2615
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      628..675
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2503..2519
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2520..2551
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0202"
                     /note=""
                     /translation="MHIISGKYKKMKLQTLDSSITRPTLTRIKEDMFNIISNYFIFENKTSLDLFGGSGSLSIEGLSRGIKFAIINDLNKDANKIISFNLKKIPTSDYVLYQKDYLELLNLLKIQHQKVDLVYLDPPFKEIDYYYVVFDFLINNNLLNDWAIIISETNQKLDLTKIKDLNLLKFKDYNKKYLYIFRLEK"
     primer_bind     complement(680..696)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(763..1351)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1522..2382)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(2383..2487)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0202"
                     /note=""
                     /translation="MHIISGKYKKMKLQTLDSSITRPTLTRIKEDMFNIISNYFIFENKTSLDLFGGSGSLSIEGLSRGIKFAIINDLNKDANKIISFNLKKIPTSDYVLYQKDYLELLNLLKIQHQKVDLVYLDPPFKEIDYYYVVFDFLINNNLLNDWAIIISETNQKLDLTKIKDLNLLKFKDYNKKYLYIFRLEK"
ORIGIN
        1 ATGCATATTA TTTCTGGTAA ATACAAGAAG ATGAAGTTGC AGACCTTAGA CAGCTCAATC
       61 ACCCGCCCCA CTCTGACGCG TATTAAGGAA GATATGTTTA ACATTATTAG TAATTATTTC
      121 ATCTTTGAAA ATAAGACCTC GTTGGATTTG TTCGGAGGAT CGGGCAGTTT ATCAATCGAA
      181 GGATTAAGCC GTGGAATCAA ATTTGCCATC ATCAATGACT TGAACAAGGA TGCGAATAAG
      241 ATTATTTCTT TTAATTTGAA GAAAATCCCT ACATCGGATT ACGTGTTGTA TCAAAAAGAT
      301 TATCTTGAAT TGCTTAATTT GCTGAAAATT CAACATCAGA AGGTGGATTT GGTCTATCTT
      361 GACCCCCCTT TCAAGGAAAT CGATTACTAT TACGTCGTAT TCGACTTTCT TATTAACAAC
      421 AACCTTCTGA ATGACTGGGC TATTATCATC AGCGAAACGA ACCAAAAATT AGATTTGACT
      481 AAGATTAAAG ACTTAAATTT GTTGAAATTC AAGGATTATA ACAAGAAGTA CTTGTATATC
      541 TTTCGTTTAG AGAAGTGAAG AGCTTAGAGA CCGGTACTAG TTCCTGCAGG GCTCGAGGTG
      601 TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC
      661 TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT GATGACACAC
      721 ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA GGCTCCGCCC
      781 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT
      841 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT
      901 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG
      961 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA
     1021 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA
     1081 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC
     1141 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG
     1201 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG
     1261 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA
     1321 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC
     1381 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG
     1441 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA
     1501 TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT
     1561 CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG
     1621 GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGAACCAC GCTCACCGGC
     1681 TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC
     1741 AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC
     1801 GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC
     1861 GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC
     1921 CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA
     1981 GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT
     2041 GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA
     2101 GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA
     2161 TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG
     2221 GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC
     2281 AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC
     2341 AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA
     2401 TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA
     2461 GAAAAATAAA CAAATAGGGG TTCCGCGCAC CTGCACCAGT CAGTAAAACG ACGGCCAGTA
     2521 GTCAAAAGCC TCCGACCGGA GGCTTTTGAC TTGGTCAGGT GGAGTGAATT CGCGGCCGCT
     2581 TCTAGAGGCT CTTCGTAATT ACGAAGAGGT CTCTA
//