LOCUS       Exported     2951 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2951
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      964..1011
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2839..2855
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2856..2887
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0203"
                     /note="Essential for recycling GMP and indirectly, cGMP."
                     /translation="MKKGKMIIISGPSGVGKGSVNGELLQNPDLRLKYSVSMTTRKPRNGEINGVNYFFVSNEEFAKAIVNDELIEYAHFVGNSYGTPRKYVEQELKKGNNVILEIEVDGATQVLNKEANVLSIFLMPPNLTELANRIRGRQTEDEEKIKARLDKALLEIPLKHNYQYVIENDNVANAVAKITDVLHLEGLTDIKTPTVYERLEQIVEQIVKEKYMYFVNNWETNVKLLAKNEEEKNKAKNFDAETYLIKLLTKKVYHKVLGHGDFSKLLDKDFVDFKIQKLMFKINFFSVEQKHYNNDEF"
     primer_bind     complement(1016..1032)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1099..1687)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1858..2718)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(2719..2823)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0203"
                     /note="Essential for recycling GMP and indirectly, cGMP."
                     /translation="MKKGKMIIISGPSGVGKGSVNGELLQNPDLRLKYSVSMTTRKPRNGEINGVNYFFVSNEEFAKAIVNDELIEYAHFVGNSYGTPRKYVEQELKKGNNVILEIEVDGATQVLNKEANVLSIFLMPPNLTELANRIRGRQTEDEEKIKARLDKALLEIPLKHNYQYVIENDNVANAVAKITDVLHLEGLTDIKTPTVYERLEQIVEQIVKEKYMYFVNNWETNVKLLAKNEEEKNKAKNFDAETYLIKLLTKKVYHKVLGHGDFSKLLDKDFVDFKIQKLMFKINFFSVEQKHYNNDEF"
ORIGIN
        1 ATGAAGAAAG GAAAGATGAT TATTATCTCA GGACCGTCGG GGGTTGGGAA GGGTTCGGTG
       61 AACGGGGAAC TGCTTCAGAA CCCGGATTTG CGCCTGAAAT ACTCTGTGAG CATGACTACC
      121 CGTAAACCCC GTAACGGAGA AATTAATGGT GTGAACTACT TCTTTGTCAG TAACGAGGAG
      181 TTTGCGAAGG CGATTGTCAA TGACGAATTA ATCGAATACG CGCATTTCGT AGGAAACAGC
      241 TACGGAACTC CCCGCAAGTA TGTAGAACAG GAGCTTAAAA AGGGGAATAA TGTAATTTTG
      301 GAGATCGAAG TGGATGGCGC AACTCAAGTT CTTAACAAAG AGGCTAATGT TCTTTCTATT
      361 TTTCTGATGC CCCCAAACTT GACAGAACTT GCCAATCGCA TTCGCGGGCG CCAGACTGAA
      421 GATGAGGAGA AAATTAAGGC ACGTCTGGAT AAAGCACTGC TGGAAATCCC ATTAAAGCAT
      481 AACTACCAGT ATGTGATCGA GAATGATAAT GTTGCTAACG CCGTAGCCAA AATTACTGAT
      541 GTCCTGCACC TGGAGGGATT GACCGATATC AAAACACCCA CCGTATACGA ACGTCTTGAA
      601 CAAATCGTGG AACAAATTGT TAAGGAAAAG TATATGTACT TTGTCAACAA TTGGGAAACG
      661 AACGTCAAGT TGTTGGCCAA AAACGAGGAA GAGAAAAACA AAGCCAAGAA TTTCGACGCA
      721 GAAACATACC TGATTAAACT GTTAACTAAA AAGGTGTATC ATAAAGTGCT GGGTCATGGT
      781 GATTTTAGCA AACTTCTGGA TAAGGATTTC GTCGATTTCA AGATTCAGAA GTTGATGTTT
      841 AAAATCAACT TCTTTTCCGT GGAGCAAAAA CACTATAACA ACGACGAGTT TTGAAGAGCT
      901 TAGAGACCGG TACTAGTTCC TGCAGGGCTC GAGGTGTCAA TCGTCGGAGC CGCTGAGCAA
      961 TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCATGGTCAT
     1021 AGCTGTTTCC TGAGAGCTTG GCAGGTGATG ACACACATTA ACAAATTTCG TGAGGAGTCT
     1081 CCAGAAGAAT GCCATTAATT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG
     1141 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC
     1201 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC
     1261 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC
     1321 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG
     1381 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC
     1441 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA
     1501 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC
     1561 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC
     1621 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG
     1681 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC
     1741 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA
     1801 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA
     1861 CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT
     1921 TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG
     1981 TGCTGCAATG ATACCGCGAG AACCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA
     2041 GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC
     2101 TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT
     2161 TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG
     2221 CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT
     2281 TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT
     2341 GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT
     2401 GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC
     2461 TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT
     2521 CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG
     2581 TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT
     2641 TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG
     2701 GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA
     2761 TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC
     2821 GCGCACCTGC ACCAGTCAGT AAAACGACGG CCAGTAGTCA AAAGCCTCCG ACCGGAGGCT
     2881 TTTGACTTGG TCAGGTGGAG TGAATTCGCG GCCGCTTCTA GAGGCTCTTC GTAATTACGA
     2941 AGAGGTCTCT A
//