LOCUS Exported 2951 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..2951 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 964..1011 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 2839..2855 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 2856..2887 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 1..0 /label="MMSYN1_0203" /note="Essential for recycling GMP and indirectly, cGMP." /translation="MKKGKMIIISGPSGVGKGSVNGELLQNPDLRLKYSVSMTTRKPRNGEINGVNYFFVSNEEFAKAIVNDELIEYAHFVGNSYGTPRKYVEQELKKGNNVILEIEVDGATQVLNKEANVLSIFLMPPNLTELANRIRGRQTEDEEKIKARLDKALLEIPLKHNYQYVIENDNVANAVAKITDVLHLEGLTDIKTPTVYERLEQIVEQIVKEKYMYFVNNWETNVKLLAKNEEEKNKAKNFDAETYLIKLLTKKVYHKVLGHGDFSKLLDKDFVDFKIQKLMFKINFFSVEQKHYNNDEF" primer_bind complement(1016..1032) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1099..1687) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1858..2718) /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" /label="bla" promoter complement(2719..2823) /label="bla promoter" /note="ampicillin resistance gene promoter" CDS complement(1..0) /label="MMSYN1_0203" /note="Essential for recycling GMP and indirectly, cGMP." /translation="MKKGKMIIISGPSGVGKGSVNGELLQNPDLRLKYSVSMTTRKPRNGEINGVNYFFVSNEEFAKAIVNDELIEYAHFVGNSYGTPRKYVEQELKKGNNVILEIEVDGATQVLNKEANVLSIFLMPPNLTELANRIRGRQTEDEEKIKARLDKALLEIPLKHNYQYVIENDNVANAVAKITDVLHLEGLTDIKTPTVYERLEQIVEQIVKEKYMYFVNNWETNVKLLAKNEEEKNKAKNFDAETYLIKLLTKKVYHKVLGHGDFSKLLDKDFVDFKIQKLMFKINFFSVEQKHYNNDEF" ORIGIN 1 ATGAAGAAAG GAAAGATGAT TATTATCTCA GGACCGTCGG GGGTTGGGAA GGGTTCGGTG 61 AACGGGGAAC TGCTTCAGAA CCCGGATTTG CGCCTGAAAT ACTCTGTGAG CATGACTACC 121 CGTAAACCCC GTAACGGAGA AATTAATGGT GTGAACTACT TCTTTGTCAG TAACGAGGAG 181 TTTGCGAAGG CGATTGTCAA TGACGAATTA ATCGAATACG CGCATTTCGT AGGAAACAGC 241 TACGGAACTC CCCGCAAGTA TGTAGAACAG GAGCTTAAAA AGGGGAATAA TGTAATTTTG 301 GAGATCGAAG TGGATGGCGC AACTCAAGTT CTTAACAAAG AGGCTAATGT TCTTTCTATT 361 TTTCTGATGC CCCCAAACTT GACAGAACTT GCCAATCGCA TTCGCGGGCG CCAGACTGAA 421 GATGAGGAGA AAATTAAGGC ACGTCTGGAT AAAGCACTGC TGGAAATCCC ATTAAAGCAT 481 AACTACCAGT ATGTGATCGA GAATGATAAT GTTGCTAACG CCGTAGCCAA AATTACTGAT 541 GTCCTGCACC TGGAGGGATT GACCGATATC AAAACACCCA CCGTATACGA ACGTCTTGAA 601 CAAATCGTGG AACAAATTGT TAAGGAAAAG TATATGTACT TTGTCAACAA TTGGGAAACG 661 AACGTCAAGT TGTTGGCCAA AAACGAGGAA GAGAAAAACA AAGCCAAGAA TTTCGACGCA 721 GAAACATACC TGATTAAACT GTTAACTAAA AAGGTGTATC ATAAAGTGCT GGGTCATGGT 781 GATTTTAGCA AACTTCTGGA TAAGGATTTC GTCGATTTCA AGATTCAGAA GTTGATGTTT 841 AAAATCAACT TCTTTTCCGT GGAGCAAAAA CACTATAACA ACGACGAGTT TTGAAGAGCT 901 TAGAGACCGG TACTAGTTCC TGCAGGGCTC GAGGTGTCAA TCGTCGGAGC CGCTGAGCAA 961 TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCATGGTCAT 1021 AGCTGTTTCC TGAGAGCTTG GCAGGTGATG ACACACATTA ACAAATTTCG TGAGGAGTCT 1081 CCAGAAGAAT GCCATTAATT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 1141 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 1201 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 1261 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 1321 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 1381 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 1441 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 1501 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 1561 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 1621 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 1681 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 1741 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 1801 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA 1861 CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT 1921 TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG 1981 TGCTGCAATG ATACCGCGAG AACCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA 2041 GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC 2101 TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT 2161 TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG 2221 CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT 2281 TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT 2341 GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT 2401 GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC 2461 TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT 2521 CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG 2581 TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT 2641 TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG 2701 GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA 2761 TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC 2821 GCGCACCTGC ACCAGTCAGT AAAACGACGG CCAGTAGTCA AAAGCCTCCG ACCGGAGGCT 2881 TTTGACTTGG TCAGGTGGAG TGAATTCGCG GCCGCTTCTA GAGGCTCTTC GTAATTACGA 2941 AGAGGTCTCT A //