LOCUS       Exported     2879 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2879
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      892..939
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2767..2783
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2784..2815
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0214"
                     /note="phosphatidylglycerophosphatase"
                     /translation="MKNKINHYYIYSGIVFIILISLFILGSVYDFKIASFKRAENLSLAVFISNLGVVIPSIPLTIALIYLIKSLKLKNKLKINNIIETLIYFIIPLVFIVFNFFYEKQSIVYSIISNLLCLIIISSLIYYFHINKEIISDPDLSIYKSIIVLITIIGLLILVNILKLTFNRPRPTNLELYRNWWNIKWTSWKHNSFPSGHTYSATTLLFVLLLFNKINKKTYLWISFTWLIIIIVAMSRIVLNKHFLTDVVFSMLLSFTILTTILIINQKKGKLFI"
     primer_bind     complement(944..960)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1027..1615)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1786..2646)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(2647..2751)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0214"
                     /note="phosphatidylglycerophosphatase"
                     /translation="MKNKINHYYIYSGIVFIILISLFILGSVYDFKIASFKRAENLSLAVFISNLGVVIPSIPLTIALIYLIKSLKLKNKLKINNIIETLIYFIIPLVFIVFNFFYEKQSIVYSIISNLLCLIIISSLIYYFHINKEIISDPDLSIYKSIIVLITIIGLLILVNILKLTFNRPRPTNLELYRNWWNIKWTSWKHNSFPSGHTYSATTLLFVLLLFNKINKKTYLWISFTWLIIIIVAMSRIVLNKHFLTDVVFSMLLSFTILTTILIINQKKGKLFI"
ORIGIN
        1 ATGAAGAACA AGATTAATCA TTACTACATC TACAGTGGGA TTGTATTCAT CATCTTGATC
       61 TCGTTGTTCA TCCTGGGGAG TGTGTATGAT TTCAAAATTG CCTCATTTAA GCGTGCCGAA
      121 AATTTATCTT TGGCCGTATT CATCTCTAAT CTTGGGGTAG TCATCCCGTC TATCCCATTG
      181 ACAATTGCAT TGATTTATCT GATTAAGAGT CTGAAACTTA AAAACAAGCT TAAAATTAAC
      241 AACATCATTG AAACCTTGAT CTACTTCATT ATCCCTCTTG TATTTATCGT ATTTAATTTT
      301 TTCTATGAAA AACAATCTAT CGTTTACTCC ATTATTTCGA ACTTATTATG TTTAATTATC
      361 ATCTCGTCTT TGATTTACTA TTTTCATATC AACAAGGAGA TTATCTCTGA CCCTGATTTA
      421 TCCATTTATA AGTCCATTAT CGTTTTAATC ACAATCATCG GGTTGTTAAT CTTAGTGAAC
      481 ATTTTAAAAC TGACATTCAA TCGCCCCCGT CCAACGAATC TGGAGTTATA CCGCAATTGG
      541 TGGAACATTA AATGGACTTC GTGGAAACAT AACTCGTTTC CTAGCGGACA TACGTACTCG
      601 GCAACTACGT TGCTGTTCGT CTTGTTATTA TTCAATAAGA TTAACAAGAA AACTTATCTG
      661 TGGATCTCTT TCACTTGGCT GATTATCATT ATTGTTGCCA TGAGCCGCAT CGTTCTGAAC
      721 AAGCACTTCC TGACTGACGT CGTATTTTCC ATGTTGCTGT CTTTCACCAT CTTGACAACA
      781 ATCTTAATCA TTAACCAGAA AAAAGGCAAA TTGTTCATTT GAAGAGCTTA GAGACCGGTA
      841 CTAGTTCCTG CAGGGCTCGA GGTGTCAATC GTCGGAGCCG CTGAGCAATA ACTAGCATAA
      901 CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC ATGGTCATAG CTGTTTCCTG
      961 AGAGCTTGGC AGGTGATGAC ACACATTAAC AAATTTCGTG AGGAGTCTCC AGAAGAATGC
     1021 CATTAATTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA
     1081 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT
     1141 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC
     1201 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT
     1261 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC
     1321 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC
     1381 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG
     1441 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC
     1501 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG
     1561 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA
     1621 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT
     1681 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG
     1741 TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT
     1801 CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC
     1861 CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT
     1921 ACCGCGAGAA CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG
     1981 GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG
     2041 CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC
     2101 TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA
     2161 ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG
     2221 TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC
     2281 ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA
     2341 CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC
     2401 AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG
     2461 TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC
     2521 CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC
     2581 AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT
     2641 ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG
     2701 CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACCTGCAC
     2761 CAGTCAGTAA AACGACGGCC AGTAGTCAAA AGCCTCCGAC CGGAGGCTTT TGACTTGGTC
     2821 AGGTGGAGTG AATTCGCGGC CGCTTCTAGA GGCTCTTCGT AATTACGAAG AGGTCTCTA
//