LOCUS       Exported     3575 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3575
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1588..1635
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3463..3479
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      3480..3511
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             1..0
                     /label="MMSYN1_0218"
                     /note="glycerol_kin: glycerol kinase"
                     /translation="MTDSKKYILTLDEGTTSARALITDKQGNIIAVEQSEFTQYFPKEGWVEHDAIEIWNTQRSALVQVLNKSGIDPSQIEAIGITNQRETAVIWNKETGLPIYNAIVWQDQRTADYCQTFDKDTLEMVKERSGLIINPYFSGTKVKWILDNVPNARQLAKDGKLMFGTINTWLIYRLTGGEVFVTDHTNAQRTLLYNIHTNDWDDELLKLFDIPRNILPEIKSCSEVYGYTFKGLFSKGNEQRIKIASSIGDQQSALFGQLCLEKGQVKVTYGTGCFILTNTGEEIVKSNHGLLTTVAYSFKDKVYYALEGSVMIAGAAVQWLRDNLRIVYNAIETEWYAGQVKDDRRVYVVPSFTGLGSPYWDSFSRGAIFGLDRGTRREHIVRATLEAIAYQANDVVDAMGKDMKKPIEIFKVDGGAANNKFLMQFQSNISQSKVIKPTNVETTAMGAAFMAGLAVGYWENVEELKKTYKVHFELTPELSKPEVDKLIKGWKVAVQRTFKWVEEIE"
     primer_bind     complement(1640..1656)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(1723..2311)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2482..3342)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3343..3447)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0218"
                     /note="glycerol_kin: glycerol kinase"
                     /translation="MTDSKKYILTLDEGTTSARALITDKQGNIIAVEQSEFTQYFPKEGWVEHDAIEIWNTQRSALVQVLNKSGIDPSQIEAIGITNQRETAVIWNKETGLPIYNAIVWQDQRTADYCQTFDKDTLEMVKERSGLIINPYFSGTKVKWILDNVPNARQLAKDGKLMFGTINTWLIYRLTGGEVFVTDHTNAQRTLLYNIHTNDWDDELLKLFDIPRNILPEIKSCSEVYGYTFKGLFSKGNEQRIKIASSIGDQQSALFGQLCLEKGQVKVTYGTGCFILTNTGEEIVKSNHGLLTTVAYSFKDKVYYALEGSVMIAGAAVQWLRDNLRIVYNAIETEWYAGQVKDDRRVYVVPSFTGLGSPYWDSFSRGAIFGLDRGTRREHIVRATLEAIAYQANDVVDAMGKDMKKPIEIFKVDGGAANNKFLMQFQSNISQSKVIKPTNVETTAMGAAFMAGLAVGYWENVEELKKTYKVHFELTPELSKPEVDKLIKGWKVAVQRTFKWVEEIE"
ORIGIN
        1 ATGACAGACT CAAAAAAGTA CATCTTAACC TTGGATGAGG GCACCACCTC TGCGCGCGCC
       61 TTGATCACCG ACAAGCAAGG GAATATCATT GCTGTGGAAC AGTCAGAATT CACGCAATAT
      121 TTTCCGAAAG AAGGCTGGGT GGAACATGAT GCGATTGAAA TCTGGAATAC TCAGCGTTCT
      181 GCTCTTGTTC AAGTGTTAAA CAAGAGTGGC ATTGATCCTT CCCAGATTGA GGCTATCGGC
      241 ATTACTAACC AGCGCGAGAC AGCAGTAATC TGGAATAAGG AAACTGGACT TCCTATTTAC
      301 AACGCAATCG TTTGGCAAGA TCAGCGCACC GCGGACTATT GTCAGACGTT TGACAAGGAT
      361 ACTCTTGAGA TGGTCAAGGA GCGCTCAGGG TTAATTATTA ACCCCTACTT CAGCGGGACG
      421 AAGGTTAAAT GGATCTTAGA TAACGTACCG AATGCGCGTC AACTTGCCAA AGACGGAAAG
      481 CTGATGTTCG GAACTATCAA CACCTGGTTG ATTTATCGCT TAACTGGCGG GGAGGTGTTT
      541 GTCACTGATC ATACAAACGC ACAACGCACG TTGCTGTATA ACATTCACAC TAACGATTGG
      601 GATGATGAAT TACTTAAGCT GTTTGATATC CCACGCAATA TCCTGCCTGA AATCAAGTCG
      661 TGCTCCGAGG TCTACGGCTA CACCTTTAAG GGATTATTCA GCAAAGGTAA TGAACAGCGC
      721 ATTAAAATTG CAAGTTCAAT TGGGGACCAA CAGTCCGCCC TTTTCGGCCA ATTGTGCTTG
      781 GAGAAAGGCC AGGTGAAAGT AACTTATGGG ACAGGTTGCT TCATCTTGAC TAATACCGGT
      841 GAGGAAATTG TGAAATCTAA TCACGGTCTT TTGACAACTG TCGCCTATTC ATTCAAGGAT
      901 AAGGTCTATT ACGCGCTGGA GGGGTCCGTT ATGATCGCAG GGGCTGCAGT ACAATGGTTA
      961 CGCGATAACT TACGCATCGT CTACAACGCA ATTGAAACGG AGTGGTATGC AGGCCAGGTG
     1021 AAAGATGACC GCCGCGTATA TGTCGTACCA TCATTTACAG GTCTTGGTTC ACCGTACTGG
     1081 GACTCCTTTT CGCGCGGGGC CATCTTTGGA CTGGACCGTG GGACCCGCCG CGAACATATT
     1141 GTTCGTGCTA CTTTAGAGGC AATCGCGTAT CAAGCCAATG ACGTCGTGGA CGCCATGGGT
     1201 AAAGACATGA AAAAGCCAAT TGAAATTTTT AAGGTGGATG GTGGGGCAGC GAATAATAAA
     1261 TTTTTAATGC AATTCCAAAG CAACATTAGC CAAAGCAAAG TCATCAAGCC TACTAATGTC
     1321 GAAACTACAG CAATGGGAGC CGCCTTTATG GCTGGGTTAG CTGTGGGCTA CTGGGAAAAT
     1381 GTCGAGGAGC TTAAAAAGAC ATATAAAGTA CATTTCGAGT TAACTCCAGA GTTATCGAAA
     1441 CCGGAGGTGG ATAAACTTAT CAAAGGATGG AAAGTTGCGG TACAACGCAC ATTCAAGTGG
     1501 GTAGAAGAGA TTGAGTGAAG AGCTTAGAGA CCGGTACTAG TTCCTGCAGG GCTCGAGGTG
     1561 TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC
     1621 TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT GATGACACAC
     1681 ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA GGCTCCGCCC
     1741 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT
     1801 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT
     1861 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG
     1921 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA
     1981 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA
     2041 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC
     2101 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG
     2161 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG
     2221 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA
     2281 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC
     2341 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG
     2401 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA
     2461 TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT
     2521 CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG
     2581 GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGAACCAC GCTCACCGGC
     2641 TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC
     2701 AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC
     2761 GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC
     2821 GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC
     2881 CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA
     2941 GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT
     3001 GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA
     3061 GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA
     3121 TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG
     3181 GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC
     3241 AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC
     3301 AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA
     3361 TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA
     3421 GAAAAATAAA CAAATAGGGG TTCCGCGCAC CTGCACCAGT CAGTAAAACG ACGGCCAGTA
     3481 GTCAAAAGCC TCCGACCGGA GGCTTTTGAC TTGGTCAGGT GGAGTGAATT CGCGGCCGCT
     3541 TCTAGAGGCT CTTCGTAATT ACGAAGAGGT CTCTA
//