LOCUS       Exported     3460 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3460
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2010..3446
                     /translation="MTKETLQKRMKRTKVITTIGPSTHSAEAIKELFNNGMTTIRLNFSHGSYEEHGYRIKAAKKISKALNKPISIILDTKGPEIRLGKFKDNKQEVVKGQSITIYTDTYSYLNKECVQGEMTVAYDMSVDLKPGDMILIDDGKLELTVDSVEPQIIKATAFNHHIVKTNKRVNLPGIDFSLPFLSEKDEKDILFGCEQGVDYIAASFVNTAENVRQIKEILNKANANHIQIISKIESQVGLDNIDEIIEESDGIMIARGDLGLEIPYYDVPYWEKIIIRKCREKGKIVIVATQMLETMTENPSPTRAEVTDVYFATELGADATMLSGESAAGDYPFLTVHTMSTINKRAEVEFYNKIYYQVQLDNARNSTSGPRAEIADLLATKTKDGEYKYAIVLSKTGELLKTISKFRPNVTILGVSPDKKLWTSFGVWHSIFMNKVDTLDNLDNNIEKLSEIAKSWGAKLGEKVLVVRSTAIKEIEVI"
                     /label="MMSYN1_0221"
                     /note=""
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGACAAAAGA AACGTTGCAA AAACGTATGA
     2041 AGCGTACGAA GGTTATTACC ACTATTGGAC CCTCCACTCA CAGCGCTGAA GCAATTAAGG
     2101 AATTATTCAA TAATGGAATG ACTACAATCC GCCTGAACTT TTCTCACGGC AGCTATGAGG
     2161 AGCACGGATA TCGTATTAAA GCAGCCAAGA AAATTAGTAA AGCGCTGAAT AAGCCGATCT
     2221 CAATCATCTT GGACACTAAG GGCCCGGAAA TCCGCCTGGG AAAGTTCAAG GACAATAAAC
     2281 AGGAGGTGGT TAAAGGGCAG AGCATTACTA TCTACACAGA CACGTATTCG TACTTGAACA
     2341 AGGAATGCGT ACAAGGCGAA ATGACGGTCG CCTACGACAT GAGTGTAGAC TTAAAACCAG
     2401 GTGACATGAT CTTGATTGAT GATGGTAAGT TAGAGTTAAC AGTAGATAGC GTGGAGCCTC
     2461 AAATCATCAA GGCGACAGCT TTTAACCATC ACATCGTAAA GACAAACAAA CGTGTTAATT
     2521 TGCCCGGAAT CGATTTCTCG CTTCCCTTTC TTTCAGAGAA GGACGAGAAG GATATTCTTT
     2581 TTGGTTGCGA ACAGGGAGTA GATTATATCG CAGCCTCTTT TGTAAATACA GCTGAAAATG
     2641 TGCGTCAGAT CAAGGAAATT CTGAATAAAG CGAACGCCAA CCATATTCAA ATCATCTCTA
     2701 AGATTGAGTC GCAAGTCGGA TTGGACAATA TCGATGAGAT CATCGAAGAA TCAGACGGCA
     2761 TTATGATCGC GCGTGGAGAC TTAGGATTGG AAATTCCGTA CTACGATGTG CCGTACTGGG
     2821 AAAAGATTAT CATTCGTAAA TGCCGTGAGA AAGGTAAAAT CGTTATCGTC GCTACTCAGA
     2881 TGTTAGAAAC CATGACGGAA AACCCGTCAC CGACCCGCGC AGAGGTCACG GACGTATACT
     2941 TCGCAACGGA GTTAGGGGCC GATGCGACAA TGTTATCAGG GGAAAGCGCA GCGGGCGACT
     3001 ACCCATTTTT AACAGTGCAC ACGATGTCTA CTATCAATAA ACGCGCTGAG GTTGAGTTCT
     3061 ACAATAAAAT TTACTACCAA GTTCAGTTGG ATAACGCACG TAATAGCACG TCCGGGCCTC
     3121 GTGCTGAAAT TGCGGACTTA TTAGCAACGA AAACGAAAGA TGGGGAATAT AAATACGCTA
     3181 TCGTCTTATC AAAGACTGGT GAATTATTAA AGACCATTAG TAAATTCCGC CCCAATGTGA
     3241 CTATTCTTGG AGTCTCCCCC GATAAGAAGC TTTGGACGAG CTTCGGTGTC TGGCACAGTA
     3301 TTTTTATGAA CAAGGTGGAC ACACTGGACA ATCTGGATAA TAACATTGAG AAGCTTTCTG
     3361 AGATTGCGAA GTCATGGGGA GCCAAGTTGG GTGAAAAAGT TTTGGTTGTT CGTTCCACTG
     3421 CTATTAAAGA GATCGAGGTG ATTTGAAGAG CTTAGAGACC
//