LOCUS Exported 3947 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3947 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 1960..2007 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 3835..3851 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 3852..3883 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 1..0 /translation="MFKVKFADIGEGLTEGTVAEVLVKVGDVVKEGQPLYFVETDKVNSEIPSPVAGKIAVINISTGQEIKVGDVVIEIDDGTSSSTTEPKVEVVEENASVVGATPVSNDVLPSRAPKPKAETKKTEQVEENASVVGATPVSNDVLPSRAPKPKAEAPKVDVQIEDTFDVCVVGAGIGGYVTAIKSAQLGLKTLIIEKEYYGGVCLNVGCIPTKTLLKTSHVYHDIMHKAKELGIVLQNTEKVVIDWAQVLQRKNGVVKKLTGGVKYLLDKNKVTQIKGEAVALDKNTISVNNKNYRVNNLIIASGSTPNHLPLPGFDQGRKDGIIIDSTRILSVPKIPETLVVIGGGVIGIEFSCLFASLGTKVTVLQGLPTILEMLDKDIIDAMTKELKNRYNIEVITNASVKEFKNGSVVYEIDGKDQMIKGEYVLESVGRKTSITGFENIGLELTPRKAIVVNEYQETNLDGVYAIGDVVGKVMLAHTAVKGAIVAANRIAKKANKDHAEDIVMDYDRIPSCIYTHPEVSMIGKTEQQLKQENIEYKTFKFPFSAIGKALADDDTSEFVKIIIEPKYKTILGAHIIGNRATEMISEIAAVIECEGTITEIANTIHPHPTMSEAIGEAAEALETGKAIHF" /label="MMSYN1_0228" /note="PdhD: lipoamide_DH: dihydrolipoyl dehydrogenase" primer_bind complement(2012..2028) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" rep_origin complement(2095..2683) /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(2854..3714) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(3715..3819) /note="ampicillin resistance gene promoter" /label="bla promoter" CDS complement(1..0) /label="MMSYN1_0228" /note="PdhD: lipoamide_DH: dihydrolipoyl dehydrogenase" /translation="MFKVKFADIGEGLTEGTVAEVLVKVGDVVKEGQPLYFVETDKVNSEIPSPVAGKIAVINISTGQEIKVGDVVIEIDDGTSSSTTEPKVEVVEENASVVGATPVSNDVLPSRAPKPKAETKKTEQVEENASVVGATPVSNDVLPSRAPKPKAEAPKVDVQIEDTFDVCVVGAGIGGYVTAIKSAQLGLKTLIIEKEYYGGVCLNVGCIPTKTLLKTSHVYHDIMHKAKELGIVLQNTEKVVIDWAQVLQRKNGVVKKLTGGVKYLLDKNKVTQIKGEAVALDKNTISVNNKNYRVNNLIIASGSTPNHLPLPGFDQGRKDGIIIDSTRILSVPKIPETLVVIGGGVIGIEFSCLFASLGTKVTVLQGLPTILEMLDKDIIDAMTKELKNRYNIEVITNASVKEFKNGSVVYEIDGKDQMIKGEYVLESVGRKTSITGFENIGLELTPRKAIVVNEYQETNLDGVYAIGDVVGKVMLAHTAVKGAIVAANRIAKKANKDHAEDIVMDYDRIPSCIYTHPEVSMIGKTEQQLKQENIEYKTFKFPFSAIGKALADDDTSEFVKIIIEPKYKTILGAHIIGNRATEMISEIAAVIECEGTITEIANTIHPHPTMSEAIGEAAEALETGKAIHF" ORIGIN 1 ATGTTTAAGG TCAAATTTGC GGATATTGGC GAGGGTTTAA CAGAAGGAAC GGTTGCCGAA 61 GTATTGGTGA AGGTCGGTGA TGTTGTTAAA GAGGGACAAC CCTTATACTT TGTGGAGACA 121 GACAAAGTCA ACAGCGAGAT TCCGTCACCG GTGGCCGGTA AGATTGCTGT TATTAACATC 181 TCTACTGGGC AGGAAATTAA GGTTGGAGAT GTGGTGATCG AGATTGATGA CGGGACGAGC 241 TCATCAACCA CGGAGCCGAA AGTGGAGGTC GTCGAAGAAA ATGCGAGCGT CGTTGGAGCA 301 ACACCAGTCA GCAATGACGT ATTGCCCTCT CGTGCCCCAA AACCTAAAGC AGAAACGAAG 361 AAAACGGAAC AGGTAGAGGA AAATGCATCA GTCGTCGGTG CTACCCCAGT ATCCAATGAC 421 GTGTTACCCA GCCGTGCGCC AAAGCCTAAG GCAGAAGCTC CGAAAGTCGA CGTTCAGATC 481 GAGGATACCT TCGACGTGTG CGTAGTGGGA GCCGGAATCG GAGGTTACGT CACTGCCATC 541 AAAAGCGCGC AACTTGGTCT TAAAACGTTA ATCATTGAGA AGGAATACTA TGGCGGCGTC 601 TGCTTGAATG TAGGTTGTAT CCCGACAAAG ACATTATTAA AGACCTCTCA CGTCTATCAC 661 GATATCATGC ACAAGGCGAA GGAATTGGGT ATTGTCTTGC AAAACACTGA AAAAGTTGTA 721 ATCGACTGGG CACAGGTTCT GCAGCGCAAA AATGGGGTAG TTAAAAAATT AACCGGGGGC 781 GTGAAGTACT TATTAGATAA GAACAAAGTA ACTCAAATTA AAGGGGAGGC CGTAGCGTTG 841 GACAAGAATA CGATCAGTGT CAACAATAAA AACTACCGTG TGAATAACCT TATTATTGCG 901 AGTGGGTCTA CGCCAAACCA CCTTCCATTG CCGGGTTTTG ATCAGGGCCG TAAAGACGGA 961 ATCATCATCG ATTCAACTCG TATCTTAAGT GTCCCGAAAA TTCCCGAAAC CCTGGTCGTG 1021 ATCGGAGGTG GGGTGATCGG GATTGAGTTC TCATGTTTAT TCGCATCTCT GGGAACCAAA 1081 GTTACTGTTC TGCAAGGGCT TCCGACGATT CTGGAGATGT TGGACAAAGA TATCATTGAC 1141 GCAATGACCA AGGAACTGAA GAATCGCTAT AACATCGAAG TCATCACGAA CGCGTCCGTT 1201 AAGGAGTTCA AGAACGGGTC TGTAGTATAC GAAATTGATG GTAAAGACCA AATGATTAAG 1261 GGGGAATACG TTCTGGAGTC AGTGGGGCGC AAGACGAGCA TCACTGGGTT TGAAAACATT 1321 GGTCTGGAAC TGACACCCCG CAAGGCCATC GTCGTAAACG AATATCAAGA AACGAATCTT 1381 GATGGAGTAT ATGCTATCGG AGATGTCGTT GGAAAAGTGA TGTTAGCTCA TACTGCAGTG 1441 AAAGGAGCTA TCGTAGCGGC GAATCGTATC GCTAAGAAGG CAAATAAGGA TCACGCCGAG 1501 GACATCGTGA TGGATTATGA CCGCATCCCT TCTTGCATCT ATACTCACCC CGAAGTCTCT 1561 ATGATTGGCA AGACTGAACA ACAGCTTAAG CAAGAAAACA TCGAATATAA AACATTCAAG 1621 TTTCCCTTTT CGGCGATCGG GAAAGCTCTT GCTGATGACG ACACCAGTGA ATTTGTGAAA 1681 ATTATTATTG AACCAAAATA CAAAACCATT TTAGGGGCGC ATATCATCGG CAATCGTGCT 1741 ACCGAGATGA TCTCTGAAAT CGCTGCAGTA ATTGAATGTG AAGGCACAAT TACAGAGATC 1801 GCTAATACCA TCCATCCCCA TCCCACCATG AGTGAGGCAA TTGGCGAGGC TGCCGAAGCC 1861 TTGGAAACGG GCAAGGCCAT TCACTTTTGA AGAGCTTAGA GACCGGTACT AGTTCCTGCA 1921 GGGCTCGAGG TGTCAATCGT CGGAGCCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC 1981 TCTAAACGGG TCTTGAGGGG TTTTTTGCAT GGTCATAGCT GTTTCCTGAG AGCTTGGCAG 2041 GTGATGACAC ACATTAACAA ATTTCGTGAG GAGTCTCCAG AAGAATGCCA TTAATTTCCA 2101 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 2161 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 2221 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 2281 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 2341 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 2401 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 2461 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 2521 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 2581 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 2641 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 2701 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 2761 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 2821 CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC 2881 TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT 2941 AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGAACC 3001 ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG 3061 AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG 3121 AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT 3181 GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG 3241 AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT 3301 TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC 3361 TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC 3421 ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA 3481 TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG 3541 AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC 3601 CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG 3661 GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT 3721 CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT 3781 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACCTGCACCA GTCAGTAAAA 3841 CGACGGCCAG TAGTCAAAAG CCTCCGACCG GAGGCTTTTG ACTTGGTCAG GTGGAGTGAA 3901 TTCGCGGCCG CTTCTAGAGG CTCTTCGTAA TTACGAAGAG GTCTCTA //