LOCUS       Exported     3947 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3947
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1960..2007
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3835..3851
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3852..3883
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /translation="MFKVKFADIGEGLTEGTVAEVLVKVGDVVKEGQPLYFVETDKVNSEIPSPVAGKIAVINISTGQEIKVGDVVIEIDDGTSSSTTEPKVEVVEENASVVGATPVSNDVLPSRAPKPKAETKKTEQVEENASVVGATPVSNDVLPSRAPKPKAEAPKVDVQIEDTFDVCVVGAGIGGYVTAIKSAQLGLKTLIIEKEYYGGVCLNVGCIPTKTLLKTSHVYHDIMHKAKELGIVLQNTEKVVIDWAQVLQRKNGVVKKLTGGVKYLLDKNKVTQIKGEAVALDKNTISVNNKNYRVNNLIIASGSTPNHLPLPGFDQGRKDGIIIDSTRILSVPKIPETLVVIGGGVIGIEFSCLFASLGTKVTVLQGLPTILEMLDKDIIDAMTKELKNRYNIEVITNASVKEFKNGSVVYEIDGKDQMIKGEYVLESVGRKTSITGFENIGLELTPRKAIVVNEYQETNLDGVYAIGDVVGKVMLAHTAVKGAIVAANRIAKKANKDHAEDIVMDYDRIPSCIYTHPEVSMIGKTEQQLKQENIEYKTFKFPFSAIGKALADDDTSEFVKIIIEPKYKTILGAHIIGNRATEMISEIAAVIECEGTITEIANTIHPHPTMSEAIGEAAEALETGKAIHF"
                     /label="MMSYN1_0228"
                     /note="PdhD: lipoamide_DH: dihydrolipoyl dehydrogenase"
     primer_bind     complement(2012..2028)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(2095..2683)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(2854..3714)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3715..3819)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0228"
                     /note="PdhD: lipoamide_DH: dihydrolipoyl dehydrogenase"
                     /translation="MFKVKFADIGEGLTEGTVAEVLVKVGDVVKEGQPLYFVETDKVNSEIPSPVAGKIAVINISTGQEIKVGDVVIEIDDGTSSSTTEPKVEVVEENASVVGATPVSNDVLPSRAPKPKAETKKTEQVEENASVVGATPVSNDVLPSRAPKPKAEAPKVDVQIEDTFDVCVVGAGIGGYVTAIKSAQLGLKTLIIEKEYYGGVCLNVGCIPTKTLLKTSHVYHDIMHKAKELGIVLQNTEKVVIDWAQVLQRKNGVVKKLTGGVKYLLDKNKVTQIKGEAVALDKNTISVNNKNYRVNNLIIASGSTPNHLPLPGFDQGRKDGIIIDSTRILSVPKIPETLVVIGGGVIGIEFSCLFASLGTKVTVLQGLPTILEMLDKDIIDAMTKELKNRYNIEVITNASVKEFKNGSVVYEIDGKDQMIKGEYVLESVGRKTSITGFENIGLELTPRKAIVVNEYQETNLDGVYAIGDVVGKVMLAHTAVKGAIVAANRIAKKANKDHAEDIVMDYDRIPSCIYTHPEVSMIGKTEQQLKQENIEYKTFKFPFSAIGKALADDDTSEFVKIIIEPKYKTILGAHIIGNRATEMISEIAAVIECEGTITEIANTIHPHPTMSEAIGEAAEALETGKAIHF"
ORIGIN
        1 ATGTTTAAGG TCAAATTTGC GGATATTGGC GAGGGTTTAA CAGAAGGAAC GGTTGCCGAA
       61 GTATTGGTGA AGGTCGGTGA TGTTGTTAAA GAGGGACAAC CCTTATACTT TGTGGAGACA
      121 GACAAAGTCA ACAGCGAGAT TCCGTCACCG GTGGCCGGTA AGATTGCTGT TATTAACATC
      181 TCTACTGGGC AGGAAATTAA GGTTGGAGAT GTGGTGATCG AGATTGATGA CGGGACGAGC
      241 TCATCAACCA CGGAGCCGAA AGTGGAGGTC GTCGAAGAAA ATGCGAGCGT CGTTGGAGCA
      301 ACACCAGTCA GCAATGACGT ATTGCCCTCT CGTGCCCCAA AACCTAAAGC AGAAACGAAG
      361 AAAACGGAAC AGGTAGAGGA AAATGCATCA GTCGTCGGTG CTACCCCAGT ATCCAATGAC
      421 GTGTTACCCA GCCGTGCGCC AAAGCCTAAG GCAGAAGCTC CGAAAGTCGA CGTTCAGATC
      481 GAGGATACCT TCGACGTGTG CGTAGTGGGA GCCGGAATCG GAGGTTACGT CACTGCCATC
      541 AAAAGCGCGC AACTTGGTCT TAAAACGTTA ATCATTGAGA AGGAATACTA TGGCGGCGTC
      601 TGCTTGAATG TAGGTTGTAT CCCGACAAAG ACATTATTAA AGACCTCTCA CGTCTATCAC
      661 GATATCATGC ACAAGGCGAA GGAATTGGGT ATTGTCTTGC AAAACACTGA AAAAGTTGTA
      721 ATCGACTGGG CACAGGTTCT GCAGCGCAAA AATGGGGTAG TTAAAAAATT AACCGGGGGC
      781 GTGAAGTACT TATTAGATAA GAACAAAGTA ACTCAAATTA AAGGGGAGGC CGTAGCGTTG
      841 GACAAGAATA CGATCAGTGT CAACAATAAA AACTACCGTG TGAATAACCT TATTATTGCG
      901 AGTGGGTCTA CGCCAAACCA CCTTCCATTG CCGGGTTTTG ATCAGGGCCG TAAAGACGGA
      961 ATCATCATCG ATTCAACTCG TATCTTAAGT GTCCCGAAAA TTCCCGAAAC CCTGGTCGTG
     1021 ATCGGAGGTG GGGTGATCGG GATTGAGTTC TCATGTTTAT TCGCATCTCT GGGAACCAAA
     1081 GTTACTGTTC TGCAAGGGCT TCCGACGATT CTGGAGATGT TGGACAAAGA TATCATTGAC
     1141 GCAATGACCA AGGAACTGAA GAATCGCTAT AACATCGAAG TCATCACGAA CGCGTCCGTT
     1201 AAGGAGTTCA AGAACGGGTC TGTAGTATAC GAAATTGATG GTAAAGACCA AATGATTAAG
     1261 GGGGAATACG TTCTGGAGTC AGTGGGGCGC AAGACGAGCA TCACTGGGTT TGAAAACATT
     1321 GGTCTGGAAC TGACACCCCG CAAGGCCATC GTCGTAAACG AATATCAAGA AACGAATCTT
     1381 GATGGAGTAT ATGCTATCGG AGATGTCGTT GGAAAAGTGA TGTTAGCTCA TACTGCAGTG
     1441 AAAGGAGCTA TCGTAGCGGC GAATCGTATC GCTAAGAAGG CAAATAAGGA TCACGCCGAG
     1501 GACATCGTGA TGGATTATGA CCGCATCCCT TCTTGCATCT ATACTCACCC CGAAGTCTCT
     1561 ATGATTGGCA AGACTGAACA ACAGCTTAAG CAAGAAAACA TCGAATATAA AACATTCAAG
     1621 TTTCCCTTTT CGGCGATCGG GAAAGCTCTT GCTGATGACG ACACCAGTGA ATTTGTGAAA
     1681 ATTATTATTG AACCAAAATA CAAAACCATT TTAGGGGCGC ATATCATCGG CAATCGTGCT
     1741 ACCGAGATGA TCTCTGAAAT CGCTGCAGTA ATTGAATGTG AAGGCACAAT TACAGAGATC
     1801 GCTAATACCA TCCATCCCCA TCCCACCATG AGTGAGGCAA TTGGCGAGGC TGCCGAAGCC
     1861 TTGGAAACGG GCAAGGCCAT TCACTTTTGA AGAGCTTAGA GACCGGTACT AGTTCCTGCA
     1921 GGGCTCGAGG TGTCAATCGT CGGAGCCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC
     1981 TCTAAACGGG TCTTGAGGGG TTTTTTGCAT GGTCATAGCT GTTTCCTGAG AGCTTGGCAG
     2041 GTGATGACAC ACATTAACAA ATTTCGTGAG GAGTCTCCAG AAGAATGCCA TTAATTTCCA
     2101 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA
     2161 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC
     2221 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC
     2281 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT
     2341 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG
     2401 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG
     2461 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA
     2521 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG
     2581 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT
     2641 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT
     2701 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG
     2761 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT
     2821 CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC
     2881 TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT
     2941 AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGAACC
     3001 ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG
     3061 AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG
     3121 AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT
     3181 GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG
     3241 AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT
     3301 TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC
     3361 TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC
     3421 ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA
     3481 TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG
     3541 AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC
     3601 CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG
     3661 GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT
     3721 CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT
     3781 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACCTGCACCA GTCAGTAAAA
     3841 CGACGGCCAG TAGTCAAAAG CCTCCGACCG GAGGCTTTTG ACTTGGTCAG GTGGAGTGAA
     3901 TTCGCGGCCG CTTCTAGAGG CTCTTCGTAA TTACGAAGAG GTCTCTA
//