LOCUS       Exported     3026 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3026
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1039..1086
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2914..2930
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2931..2962
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             1..0
                     /label="MMSYN1_0229"
                     /note=""
                     /translation="MYTLEEIKNQLVLKSEKKSIVFPEGESEIIQSVAKTLVDEKLGLPILLFKSSKEVPSEIKNNSSIKTICLDEFDTKEFSEEFVKLRKGKATIEVAHQVMQLPNYVGAMLVKLNQADCMLSGLNNTTADTIRPALQIIGTKPGYNIASSIFIMSKGDENYIFTDCALNIKPTSEQLVEITQMAVDFAKTLNVKNVEAALLSYSTNGSGKGEDVDRVHKAVEILKSSQNDYVCEGEIQFDAAFDKKTRDKKFKNCLLTKQTPDIFVFPDINAGNIGYKIAQRMGGFEAIGPFVLGLNQPVNDLSRGATFIDVLNTAIMTLHLSY"
     primer_bind     complement(1091..1107)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1174..1762)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1933..2793)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2794..2898)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0229"
                     /note=""
                     /translation="MYTLEEIKNQLVLKSEKKSIVFPEGESEIIQSVAKTLVDEKLGLPILLFKSSKEVPSEIKNNSSIKTICLDEFDTKEFSEEFVKLRKGKATIEVAHQVMQLPNYVGAMLVKLNQADCMLSGLNNTTADTIRPALQIIGTKPGYNIASSIFIMSKGDENYIFTDCALNIKPTSEQLVEITQMAVDFAKTLNVKNVEAALLSYSTNGSGKGEDVDRVHKAVEILKSSQNDYVCEGEIQFDAAFDKKTRDKKFKNCLLTKQTPDIFVFPDINAGNIGYKIAQRMGGFEAIGPFVLGLNQPVNDLSRGATFIDVLNTAIMTLHLSY"
ORIGIN
        1 ATGTACACCT TGGAAGAGAT TAAAAATCAA TTAGTATTAA AGTCTGAGAA AAAGTCCATC
       61 GTGTTCCCAG AGGGGGAATC CGAAATTATC CAGAGTGTCG CAAAGACTCT TGTAGACGAG
      121 AAGCTGGGAC TGCCCATTTT GTTGTTTAAA TCTAGTAAGG AAGTGCCGTC CGAAATCAAG
      181 AATAATAGCT CCATTAAGAC CATCTGTCTG GACGAATTTG ACACGAAAGA GTTTTCCGAG
      241 GAATTTGTGA AGCTGCGTAA AGGAAAAGCA ACTATCGAGG TAGCGCATCA AGTCATGCAG
      301 CTGCCAAATT ATGTCGGTGC AATGCTTGTG AAATTGAACC AGGCCGACTG CATGCTTAGT
      361 GGATTGAATA ACACGACAGC AGATACAATT CGCCCGGCCT TGCAGATCAT CGGAACCAAG
      421 CCGGGTTACA ACATTGCGTC GAGTATTTTT ATCATGTCCA AAGGTGACGA GAACTACATC
      481 TTCACCGACT GTGCGCTGAA CATCAAGCCA ACATCTGAAC AGTTAGTGGA GATCACACAG
      541 ATGGCGGTAG ATTTCGCAAA AACCCTGAAT GTAAAAAATG TTGAAGCTGC CCTTCTTTCA
      601 TACTCTACTA ATGGATCTGG TAAGGGGGAG GACGTTGACC GCGTGCACAA AGCCGTGGAG
      661 ATCCTGAAAT CCTCTCAAAA TGACTACGTC TGCGAGGGAG AAATTCAATT TGATGCTGCT
      721 TTTGACAAAA AGACCCGTGA CAAAAAATTC AAGAATTGTT TATTGACAAA ACAAACACCA
      781 GACATCTTCG TTTTCCCCGA CATTAACGCG GGCAACATTG GTTATAAAAT CGCCCAACGC
      841 ATGGGAGGAT TTGAGGCTAT TGGCCCCTTC GTCTTGGGCC TGAATCAGCC AGTAAACGAT
      901 CTTTCGCGCG GCGCCACGTT TATTGATGTT CTTAATACAG CTATTATGAC CTTGCATCTG
      961 TCCTACTGAA GAGCTTAGAG ACCGGTACTA GTTCCTGCAG GGCTCGAGGT GTCAATCGTC
     1021 GGAGCCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT
     1081 TTTTTGCATG GTCATAGCTG TTTCCTGAGA GCTTGGCAGG TGATGACACA CATTAACAAA
     1141 TTTCGTGAGG AGTCTCCAGA AGAATGCCAT TAATTTCCAT AGGCTCCGCC CCCCTGACGA
     1201 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA
     1261 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC
     1321 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG
     1381 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC
     1441 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG
     1501 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT
     1561 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT
     1621 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG
     1681 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC
     1741 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA
     1801 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC
     1861 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC
     1921 TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT
     1981 TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT
     2041 ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGAACCA CGCTCACCGG CTCCAGATTT
     2101 ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC
     2161 CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA
     2221 TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG
     2281 TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT
     2341 GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC
     2401 AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT
     2461 AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG
     2521 GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC
     2581 TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC
     2641 GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT
     2701 TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG
     2761 AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG
     2821 CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA
     2881 ACAAATAGGG GTTCCGCGCA CCTGCACCAG TCAGTAAAAC GACGGCCAGT AGTCAAAAGC
     2941 CTCCGACCGG AGGCTTTTGA CTTGGTCAGG TGGAGTGAAT TCGCGGCCGC TTCTAGAGGC
     3001 TCTTCGTAAT TACGAAGAGG TCTCTA
//