LOCUS       Exported     3239 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3239
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1252..1299
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3127..3143
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3144..3175
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             1..0
                     /label="MMSYN1_0230"
                     /note="AckA: acetate kinase"
                     /translation="MILVINSGSSSIKFKLFDTSKTIEPILDGLAERIGIDGFLKFEHNNQKYKFEDTLPDHEHAIQLILNKLLELKIISNIDEINGVGFRVVHGGEISHSSIITDEILSKIQDSVKLAPLHNPAAIIAIKAVKKLMPNTNMVACFDTAFHQTMPEVNYLYTVPYKWYEEFGVRKYGFHGISYEYIVNKSSEILNKKKENLNLIVCHLGNGASISCIKDGKSYDTSMGLTPLAGLMMGTRSGDIDVSICEYIAKQTNTDIFTITQTLNKQSGLLGLSQVSADMRDVLEQYDKNDKKAVIAVEKYVQIVADFIVKYANYLDSIDAVVFTAGIGENADVIRDLICKKVKLLDLQIDQDKNQAKYSDYKLISSEESKIPVYAIRTNEEKMICLDTLNLIK"
     primer_bind     complement(1304..1320)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(1387..1975)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2146..3006)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3007..3111)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /translation="MILVINSGSSSIKFKLFDTSKTIEPILDGLAERIGIDGFLKFEHNNQKYKFEDTLPDHEHAIQLILNKLLELKIISNIDEINGVGFRVVHGGEISHSSIITDEILSKIQDSVKLAPLHNPAAIIAIKAVKKLMPNTNMVACFDTAFHQTMPEVNYLYTVPYKWYEEFGVRKYGFHGISYEYIVNKSSEILNKKKENLNLIVCHLGNGASISCIKDGKSYDTSMGLTPLAGLMMGTRSGDIDVSICEYIAKQTNTDIFTITQTLNKQSGLLGLSQVSADMRDVLEQYDKNDKKAVIAVEKYVQIVADFIVKYANYLDSIDAVVFTAGIGENADVIRDLICKKVKLLDLQIDQDKNQAKYSDYKLISSEESKIPVYAIRTNEEKMICLDTLNLIK"
                     /label="MMSYN1_0230"
                     /note="AckA: acetate kinase"
ORIGIN
        1 ATGATTTTGG TCATTAATAG CGGTTCGTCC TCTATTAAAT TCAAACTGTT TGATACCTCG
       61 AAAACCATTG AGCCAATTCT GGACGGGCTG GCGGAGCGTA TTGGAATCGA CGGATTTTTA
      121 AAATTTGAAC ACAATAACCA AAAATACAAG TTTGAAGATA CTCTGCCAGA CCACGAGCAT
      181 GCAATTCAAC TGATCTTAAA TAAGCTGTTA GAATTGAAGA TCATTTCTAA TATTGATGAA
      241 ATTAACGGCG TGGGGTTCCG CGTAGTGCAT GGAGGCGAGA TTTCCCACTC TTCGATTATT
      301 ACGGATGAGA TTTTATCTAA GATCCAAGAT TCTGTCAAGT TGGCACCTCT GCATAACCCG
      361 GCGGCGATCA TTGCTATTAA AGCGGTAAAG AAGTTGATGC CCAATACGAA CATGGTAGCG
      421 TGCTTCGATA CAGCGTTTCA TCAAACAATG CCTGAAGTAA ACTATTTATA CACAGTGCCT
      481 TACAAGTGGT ACGAAGAATT TGGCGTCCGC AAATATGGAT TTCACGGAAT CAGTTACGAG
      541 TACATTGTTA ACAAAAGTAG TGAAATTTTG AATAAAAAGA AGGAAAATCT TAACTTGATC
      601 GTATGCCATT TGGGCAACGG GGCATCGATC AGTTGTATTA AAGATGGAAA ATCGTACGAC
      661 ACTAGCATGG GCTTAACCCC GCTGGCTGGC TTGATGATGG GGACACGTTC TGGAGATATC
      721 GACGTATCCA TCTGCGAGTA TATTGCGAAG CAGACTAACA CAGATATCTT CACCATTACC
      781 CAGACACTTA ACAAACAGAG CGGTTTGTTG GGATTGAGCC AGGTGAGTGC GGACATGCGC
      841 GACGTCCTTG AGCAATATGA TAAGAACGAT AAAAAGGCGG TGATCGCAGT GGAGAAGTAT
      901 GTACAAATCG TCGCGGACTT TATTGTAAAG TACGCTAACT ACTTAGATTC CATTGATGCT
      961 GTAGTGTTCA CGGCCGGCAT TGGCGAAAAT GCAGATGTGA TCCGTGATCT TATTTGTAAG
     1021 AAGGTCAAAT TGTTGGATTT ACAGATCGAT CAAGACAAGA ATCAGGCTAA ATATAGCGAC
     1081 TATAAACTGA TTTCGTCAGA GGAATCGAAA ATCCCAGTGT ATGCGATTCG TACCAATGAA
     1141 GAGAAAATGA TTTGTTTGGA CACGCTTAAT CTTATTAAGT GAAGAGCTTA GAGACCGGTA
     1201 CTAGTTCCTG CAGGGCTCGA GGTGTCAATC GTCGGAGCCG CTGAGCAATA ACTAGCATAA
     1261 CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC ATGGTCATAG CTGTTTCCTG
     1321 AGAGCTTGGC AGGTGATGAC ACACATTAAC AAATTTCGTG AGGAGTCTCC AGAAGAATGC
     1381 CATTAATTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA
     1441 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT
     1501 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC
     1561 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT
     1621 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC
     1681 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC
     1741 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG
     1801 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC
     1861 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG
     1921 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA
     1981 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT
     2041 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG
     2101 TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT
     2161 CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC
     2221 CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT
     2281 ACCGCGAGAA CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG
     2341 GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG
     2401 CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC
     2461 TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA
     2521 ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG
     2581 TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC
     2641 ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA
     2701 CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC
     2761 AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG
     2821 TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC
     2881 CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC
     2941 AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT
     3001 ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG
     3061 CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACCTGCAC
     3121 CAGTCAGTAA AACGACGGCC AGTAGTCAAA AGCCTCCGAC CGGAGGCTTT TGACTTGGTC
     3181 AGGTGGAGTG AATTCGCGGC CGCTTCTAGA GGCTCTTCGT AATTACGAAG AGGTCTCTA
//