LOCUS       Exported     3745 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3745
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2010..3731
                     /label="MMSYN1_0233"
                     /note="General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. Enzyme I transfers the phosphoryl group from phosphoenolpyruvate (PEP) to the phosphoryl carrier protein (HPr)."
                     /translation="MSKQIKGIAASDGISLAKALVIKEIKLDVQKQLIDDVDQQIAKLEQAINQTITDLKKIQKITLEKLGEEKAAIFDAHQDIANDPAIKEEVVQLIKTEKVNAEYALFIVSNNYFEMFSQLEDPYFKERSADIKDVSSRIISHILGLEIHDLSTIDKEVIIISDDLTPSQTAQLDKKFVKGFLTNVGGRTSHAAIMARSLEIPAILGLKNITELVKTDDLIALDGSSGIVELDLNDNDIKNYQTKVKQYLELKDQLKKFKDKPSLTKDKIKKLIEANIGSTNDIQSVLDSGAEGIGLFRTEFLYMNNDHFPTEEEQFEAYKKVVSQIDHLVVFRTLDIGGDKKLSYFKFDEEMNPFLGYRAIRFTLDRKDIFKDQIRALLRASAFGKLGIMFPMIATIDEFKQAKAFVEECKLELDKENIKYDKQVQIGMMVEIPSAAILADQFAKYADFFSIGTNDLIQYSFASDRMNQNVSYLYQPLNPSLLRLIQLTISGAHKHNKWVGMCGEMAGDSKALPILLGLDLDAFSMSATSVLKARSLMSKIESIKAKELANKALECQTSQQVNDLVEEFLKNLD"
     primer_bind     complement(90..106)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(173..761)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGTCGAAACA AATCAAAGGA ATTGCTGCAT
     2041 CTGATGGGAT TTCCTTAGCC AAGGCATTAG TCATTAAAGA GATTAAATTG GACGTTCAGA
     2101 AACAGTTGAT CGATGACGTA GACCAGCAAA TTGCAAAACT GGAACAAGCT ATTAATCAGA
     2161 CAATCACAGA CCTGAAGAAG ATTCAAAAAA TCACTCTTGA GAAATTAGGT GAGGAGAAAG
     2221 CCGCAATTTT CGATGCGCAC CAGGATATTG CAAACGATCC GGCCATCAAA GAAGAGGTCG
     2281 TACAATTAAT TAAAACAGAG AAGGTAAACG CCGAGTACGC ACTGTTTATT GTTTCAAACA
     2341 ACTATTTCGA AATGTTTAGT CAATTGGAGG ACCCTTATTT TAAAGAGCGC AGCGCGGATA
     2401 TTAAAGATGT TTCTTCCCGT ATTATTTCCC ATATTTTGGG ATTAGAAATC CATGATCTGT
     2461 CGACTATCGA TAAAGAAGTC ATTATTATTT CAGATGATCT GACTCCCAGC CAGACGGCCC
     2521 AACTGGACAA GAAATTCGTC AAGGGGTTCC TTACCAACGT GGGCGGACGC ACTTCGCACG
     2581 CCGCCATTAT GGCTCGCTCC TTAGAGATTC CTGCCATCTT GGGTCTTAAG AACATTACGG
     2641 AGCTTGTTAA AACGGACGAT CTGATTGCTC TTGATGGCTC TAGCGGTATT GTGGAGCTGG
     2701 ACCTGAATGA TAATGACATC AAAAATTATC AAACAAAGGT AAAGCAGTAC CTTGAACTTA
     2761 AGGACCAGCT TAAGAAATTT AAGGACAAAC CCAGTTTGAC CAAGGACAAG ATCAAGAAGT
     2821 TAATCGAAGC GAACATCGGT TCAACAAATG ATATTCAATC TGTTCTTGAC AGCGGGGCGG
     2881 AGGGAATCGG ACTGTTTCGC ACTGAATTTT TGTATATGAA CAATGACCAT TTTCCCACTG
     2941 AGGAGGAACA ATTCGAGGCA TACAAGAAGG TCGTCAGCCA AATTGACCAT CTTGTAGTGT
     3001 TCCGTACCTT AGATATTGGT GGTGATAAGA AATTGTCATA TTTTAAGTTC GACGAGGAGA
     3061 TGAACCCCTT TCTTGGGTAT CGCGCGATCC GTTTCACGCT GGACCGTAAG GACATCTTTA
     3121 AAGATCAAAT TCGCGCGTTA CTTCGCGCAT CGGCGTTCGG CAAGTTAGGC ATCATGTTTC
     3181 CGATGATTGC CACAATCGAT GAATTTAAGC AGGCTAAAGC CTTTGTTGAG GAGTGTAAGT
     3241 TAGAACTGGA CAAGGAGAAT ATTAAATACG ACAAACAGGT TCAGATTGGG ATGATGGTAG
     3301 AGATTCCCAG TGCGGCCATC TTGGCAGACC AATTTGCTAA GTACGCGGAC TTCTTCAGCA
     3361 TTGGCACCAA TGACTTGATC CAATATTCAT TTGCATCTGA TCGTATGAAC CAAAACGTGT
     3421 CCTATTTATA CCAACCATTG AACCCAAGCC TGTTACGCCT GATCCAACTG ACCATCAGTG
     3481 GAGCGCATAA ACATAATAAA TGGGTAGGAA TGTGCGGTGA GATGGCTGGA GACAGTAAAG
     3541 CTTTGCCGAT TCTGCTGGGA TTGGATTTAG ATGCGTTCTC TATGTCTGCA ACCTCAGTGT
     3601 TAAAGGCGCG CTCGTTGATG TCGAAGATCG AGTCAATCAA GGCAAAGGAG CTGGCGAACA
     3661 AAGCTTTAGA GTGCCAAACT TCACAACAGG TGAATGATTT GGTCGAAGAG TTTCTTAAGA
     3721 ACTTAGATTG AAGAGCTTAG AGACC
//