LOCUS       Exported     3811 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3811
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2010..3797
                     /label="MMSYN1_0239"
                     /note=""
                     /translation="MWFELMLIITKLSETKAINIVFLTIFLLAFFCSLFTIFKLYVYRNTLKKLHFTFLNIEKTLKHPLANRLVRMQFIVTNSNNQNLSKALEIWKIKYNQIYNVELDILIKQTKEHFDLNSYSKKILFRVLSIKNFYRTRKLYKTSKAIYQKVNLMYSETQQVTNIEFLLRDYRIILQNHINDLFDIVFKEQENNELNIDKKIINNYQESIFKKMIVCEYYIKIGNFKEAFSKLNLLSNNVIEYIKFLDDHYKITKFLEFNGILDSKLQEIKNKVQLDVNQKNNQLIKYQINLLEQQFIDQKQAVEKLLFHGKNNQAFLIIETLIKNIQNLDVILKYDQQILSLFETNVKNIRTILLSFNTELLKTEELINFNNNLNNDISDIKIQFDQLKTSFNNITTEFDKEYQKISSNFIQFNSLIVDYVNYIRNVLIDIKKHYTQLIDIKTLLKNKSLVLRDLETKYDNIKTLLFLSQAIIKKYEKVINWSVYKELINNKFLIINFIYKNLELEANTFTNDYDALLVLNNQLDNQIEQVEQLHLNIEQVVVIYKIAQQIIIYIAKNLAYISNNNAFEEILTKFKEKNHKKVINLAIHLIRKNQL"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGTGGTTTGA ATTAATGCTG ATCATTACAA
     2041 AACTGTCCGA AACGAAAGCT ATTAATATCG TGTTCTTGAC GATCTTTTTG TTGGCATTCT
     2101 TCTGCTCTTT GTTTACTATT TTTAAACTGT ACGTCTATCG TAATACTCTT AAGAAGCTTC
     2161 ATTTCACATT TCTTAATATC GAGAAAACAC TGAAGCATCC TCTGGCCAAC CGCTTGGTAC
     2221 GCATGCAATT TATTGTCACG AACTCAAACA ATCAAAACTT GTCTAAGGCG TTAGAGATTT
     2281 GGAAGATCAA GTATAACCAG ATTTATAATG TGGAACTGGA CATCTTAATC AAGCAGACCA
     2341 AAGAGCATTT CGATCTGAAT AGTTACTCGA AGAAAATCCT GTTTCGCGTG TTATCGATTA
     2401 AAAACTTCTA CCGCACGCGT AAACTTTATA AAACTAGCAA AGCGATCTAC CAGAAAGTAA
     2461 ATCTTATGTA TAGTGAGACT CAACAAGTCA CGAACATTGA ATTCCTTCTG CGCGATTACC
     2521 GCATTATCTT ACAAAACCAT ATTAATGACT TGTTCGACAT TGTATTCAAA GAGCAGGAAA
     2581 ATAACGAGCT GAATATCGAT AAAAAGATTA TTAACAATTA CCAAGAAAGC ATCTTTAAAA
     2641 AGATGATCGT GTGCGAATAT TACATTAAAA TCGGAAACTT TAAAGAAGCT TTCAGTAAAC
     2701 TGAACCTTTT GAGCAACAAC GTTATCGAAT ATATTAAATT TTTAGATGAT CATTACAAAA
     2761 TCACTAAGTT TTTGGAGTTT AACGGTATTT TAGATTCGAA ACTGCAAGAG ATTAAAAACA
     2821 AAGTGCAACT GGACGTCAAC CAAAAGAACA ACCAGCTTAT TAAGTATCAG ATCAATTTAC
     2881 TTGAGCAGCA ATTCATTGAC CAGAAACAAG CAGTAGAAAA GCTGTTATTC CACGGCAAAA
     2941 ATAACCAAGC GTTTCTGATT ATTGAAACTT TAATCAAAAA TATTCAAAAT TTAGATGTAA
     3001 TCCTTAAGTA TGACCAGCAG ATTCTTAGCC TTTTCGAGAC TAATGTTAAA AACATTCGTA
     3061 CAATCCTTTT AAGCTTCAAT ACTGAATTGT TAAAAACTGA AGAGTTGATT AACTTTAACA
     3121 ACAACTTAAA CAATGACATT TCTGACATTA AAATCCAGTT TGATCAACTT AAGACCTCCT
     3181 TCAACAACAT TACTACTGAA TTTGATAAAG AGTACCAAAA AATCTCTTCG AATTTCATTC
     3241 AGTTCAATTC GCTGATTGTA GATTATGTCA ATTACATTCG CAATGTCCTG ATTGACATTA
     3301 AGAAACACTA CACCCAATTA ATTGATATTA AGACGCTTTT GAAGAATAAG TCTCTTGTTC
     3361 TTCGTGACTT GGAAACAAAA TATGATAACA TCAAAACCTT ACTGTTTTTA AGCCAGGCCA
     3421 TTATCAAAAA GTACGAAAAG GTCATCAATT GGTCGGTGTA TAAAGAGCTG ATCAACAACA
     3481 AATTTCTTAT TATCAATTTT ATCTACAAAA ACTTGGAGTT AGAAGCGAAT ACCTTCACAA
     3541 ATGACTATGA CGCCTTACTT GTCCTGAATA ACCAGTTGGA CAACCAGATC GAACAGGTTG
     3601 AACAGTTGCA TTTAAACATC GAACAGGTGG TGGTGATTTA TAAAATCGCA CAGCAAATTA
     3661 TCATCTATAT TGCCAAAAAT CTTGCCTACA TTTCTAATAA CAATGCCTTT GAAGAAATCT
     3721 TGACCAAATT CAAGGAAAAG AACCATAAGA AGGTCATTAA TTTGGCAATC CACCTGATTC
     3781 GCAAGAACCA GCTGTGAAGA GCTTAGAGAC C
//