LOCUS       Exported     3245 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3245
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      1258..1305
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3133..3149
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3150..3181
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0240"
                     /note="ThiI: Probable tRNA sulfurtransferase, ThiS sulfurtransferase"
                     /translation="MKYILIRYGELTLKGNNRFQFIDKLISNIKFKLKQFDKEDIKFIKDHNSLTLEIKDELETQIVEQLKTVFGIYSISIINYVNKDLEQIKKTVLQIAKNSKTKTFKLEVSRKDKSFNYTSIELKQILATEILKNTNHLSVDVHNPELVIEIVVKKDHVDVFDNRIDGLKGLPVGISDKGLCLLSGGIDSPVSAFLTLKRGMQVDFIHFMTPPHTSYQALDKVFNLAKKIAKYNLSNFKLHICDFNLLLQELQHLIDPSYKITIMRRMFLRIANIIAKNNNNKAIITGESLGQVASQTIQSINVINNVSDLPILRPVITYDKEEIIKIAKFIDTYETSILPFDDVCSMFVPKDPIIKPKLDIATKLEENIYWKELLEETIKNNIKTFVYKNGEFAQE"
     primer_bind     complement(1310..1326)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1393..1981)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2152..3012)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3013..3117)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0240"
                     /note="ThiI: Probable tRNA sulfurtransferase, ThiS sulfurtransferase"
                     /translation="MKYILIRYGELTLKGNNRFQFIDKLISNIKFKLKQFDKEDIKFIKDHNSLTLEIKDELETQIVEQLKTVFGIYSISIINYVNKDLEQIKKTVLQIAKNSKTKTFKLEVSRKDKSFNYTSIELKQILATEILKNTNHLSVDVHNPELVIEIVVKKDHVDVFDNRIDGLKGLPVGISDKGLCLLSGGIDSPVSAFLTLKRGMQVDFIHFMTPPHTSYQALDKVFNLAKKIAKYNLSNFKLHICDFNLLLQELQHLIDPSYKITIMRRMFLRIANIIAKNNNNKAIITGESLGQVASQTIQSINVINNVSDLPILRPVITYDKEEIIKIAKFIDTYETSILPFDDVCSMFVPKDPIIKPKLDIATKLEENIYWKELLEETIKNNIKTFVYKNGEFAQE"
ORIGIN
        1 ATGAAGTATA TTTTGATCCG TTACGGCGAG CTTACATTAA AGGGTAATAA TCGTTTCCAG
       61 TTTATTGATA AATTAATTTC CAATATCAAG TTTAAGTTGA AACAATTCGA TAAAGAGGAT
      121 ATTAAATTTA TTAAAGATCA CAACAGCCTT ACTCTGGAGA TCAAGGACGA GTTGGAGACT
      181 CAAATCGTAG AACAATTAAA AACGGTGTTC GGTATCTACA GTATCTCTAT CATTAACTAT
      241 GTGAACAAGG ATCTGGAACA AATCAAGAAA ACAGTTCTTC AGATCGCCAA GAACAGTAAG
      301 ACAAAGACGT TTAAGCTGGA AGTTAGCCGC AAAGACAAGT CATTCAACTA TACATCGATT
      361 GAGCTTAAGC AAATCCTGGC AACAGAGATT CTGAAGAATA CGAACCATCT GAGTGTCGAT
      421 GTGCATAACC CGGAACTTGT AATTGAAATT GTTGTAAAGA AAGATCATGT CGATGTTTTT
      481 GATAACCGCA TCGACGGTTT AAAAGGTCTG CCGGTCGGCA TTAGCGATAA AGGGCTTTGT
      541 TTATTATCAG GTGGCATTGA CTCACCGGTA TCTGCATTTC TGACTCTGAA ACGTGGTATG
      601 CAAGTCGATT TTATTCACTT CATGACCCCG CCCCACACTT CATATCAAGC ACTTGATAAA
      661 GTTTTCAACT TAGCCAAGAA AATTGCCAAA TATAATTTGT CGAACTTCAA ACTGCATATT
      721 TGTGATTTTA ATCTGCTTCT TCAAGAGTTG CAGCATCTGA TCGATCCAAG TTACAAGATT
      781 ACGATTATGC GTCGCATGTT TTTACGTATC GCAAATATCA TTGCAAAGAA TAACAACAAT
      841 AAGGCTATTA TCACAGGGGA ATCTCTGGGA CAAGTCGCCT CTCAGACGAT CCAGTCCATT
      901 AATGTCATTA ACAACGTATC AGATCTGCCA ATTTTGCGTC CTGTCATCAC GTATGATAAA
      961 GAGGAGATTA TCAAGATTGC CAAGTTCATT GACACCTATG AAACATCGAT TTTGCCATTT
     1021 GACGATGTTT GTAGTATGTT CGTACCGAAG GATCCTATTA TCAAACCCAA ACTTGACATT
     1081 GCAACTAAAC TGGAGGAGAA TATCTATTGG AAAGAGCTTC TTGAGGAAAC CATCAAAAAC
     1141 AACATTAAGA CGTTTGTGTA CAAAAATGGC GAATTTGCTC AAGAGTGAAG AGCTTAGAGA
     1201 CCGGTACTAG TTCCTGCAGG GCTCGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA
     1261 GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT
     1321 TTCCTGAGAG CTTGGCAGGT GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA
     1381 GAATGCCATT AATTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC
     1441 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG
     1501 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT
     1561 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA
     1621 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC
     1681 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC
     1741 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT
     1801 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT
     1861 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC
     1921 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA
     1981 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
     2041 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
     2101 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
     2161 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     2221 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     2281 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     2341 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     2401 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     2461 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     2521 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     2581 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     2641 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     2701 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     2761 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     2821 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     2881 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     2941 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     3001 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     3061 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     3121 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     3181 TTGGTCAGGT GGAGTGAATT CGCGGCCGCT TCTAGAGGCT CTTCGTAATT ACGAAGAGGT
     3241 CTCTA
//