LOCUS       Exported     3778 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3778
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             2010..3764
                     /note="uvrC: excinuclease ABC subunit C"
                     /translation="MSLKQQVDLLPNKPGCYLFFNKDNDVIYVGKAKNLKKRVSTYFNKAYNIKTTRLVREITDLKYFIVDNEKESLLLEKNLIKKYHPKYNVLLNDDKTYPYIIITNQKDPMYKYVRKYDKKALKNYGPLPIGSNARSILLTLQRLFPLRMCQGNLNKPCLYYHLNQCSGACFKQVDLSYYEYQIKQVDKFFKGEINQVKQTLIKQMQKASDNLQFEQAQRIKDQITSLDFITAKQNVDIVTNKNIDVINYEINQDKICFVMLFYRLGQLTYKDEYIQNYEGQNLSELFNSYLQQIYQKNIYPDVLLIPNEIELLDLDQNLLEFSSYSLNKQDDVFIKLAKQNAIDSLNKSVISHNVNSGDEIEILEQLKQISNASKYLKRIEIFDISNIYNQFITGACIVYINAKPIRNEFRKYNIDSSYTSDYARMKFMLEKRFLKRIKEKEQLPDLIIVDGGIIQIHAAKEVLNKLNLKIDVIGLSKDDHHKTRYLIDIFEQTIDIKNFKKLYNFLTSLQIRVDEYAKSGFRKKYHNQLNDQILLIKGVGKKTNLKLYKHFKTIDNIKNASFDELNKVINNKKITNLIISNLNK"
                     /label="MMSYN1_0254"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGTCCCTTAA GCAGCAAGTT GATCTGTTAC
     2041 CCAACAAACC AGGCTGTTAC TTATTCTTCA ATAAAGACAA CGATGTCATT TACGTCGGAA
     2101 AAGCCAAAAA TCTTAAGAAA CGTGTATCCA CGTACTTTAA CAAGGCCTAT AATATTAAGA
     2161 CGACCCGTTT GGTACGCGAA ATCACTGACT TGAAGTATTT CATCGTCGAC AATGAGAAGG
     2221 AGTCATTGTT GTTAGAGAAA AATTTGATCA AGAAATATCA TCCTAAATAC AATGTACTTC
     2281 TTAACGATGA TAAAACGTAT CCATATATTA TTATCACCAA CCAGAAGGAC CCCATGTATA
     2341 AGTACGTGCG TAAGTATGAC AAGAAAGCAC TTAAAAATTA TGGCCCGTTA CCAATTGGGT
     2401 CAAATGCCCG TTCAATTCTG CTTACATTAC AACGTTTATT CCCCCTGCGC ATGTGCCAAG
     2461 GGAATTTAAA CAAACCGTGC CTGTATTATC ACCTTAATCA GTGTTCGGGT GCATGCTTTA
     2521 AACAAGTCGA CCTTAGCTAC TACGAATACC AAATCAAACA GGTGGACAAA TTCTTTAAAG
     2581 GTGAGATCAA TCAAGTAAAA CAGACGTTGA TCAAGCAGAT GCAAAAAGCC AGTGACAATT
     2641 TACAGTTTGA GCAGGCACAA CGCATTAAGG ACCAGATTAC CTCCTTGGAT TTCATCACCG
     2701 CTAAGCAAAA TGTAGATATC GTAACAAACA AAAATATTGA TGTAATTAAT TATGAGATTA
     2761 ACCAGGATAA AATCTGTTTT GTGATGCTTT TTTACCGTTT AGGCCAATTG ACTTACAAAG
     2821 ACGAGTATAT TCAAAACTAC GAGGGCCAAA ATCTGTCGGA GCTTTTCAAT TCGTACTTAC
     2881 AACAGATCTA TCAGAAGAAC ATCTATCCGG ACGTGTTATT AATTCCAAAC GAGATCGAAT
     2941 TGTTGGACTT AGATCAAAAC CTGTTGGAAT TCTCGTCTTA TTCATTAAAT AAACAGGACG
     3001 ATGTGTTTAT TAAATTGGCA AAGCAGAACG CAATCGACTC ATTGAACAAG TCGGTAATTT
     3061 CACATAACGT AAACTCAGGA GATGAGATTG AGATTCTTGA GCAGTTGAAA CAAATTTCAA
     3121 ACGCCAGCAA ATACCTGAAG CGCATCGAAA TCTTCGATAT TAGTAACATC TACAATCAAT
     3181 TTATTACCGG CGCTTGCATC GTGTACATTA ACGCAAAACC TATCCGTAAC GAATTTCGCA
     3241 AGTATAATAT CGATAGCAGC TACACCTCAG ACTATGCGCG TATGAAATTT ATGTTAGAGA
     3301 AACGTTTTTT GAAGCGCATC AAGGAAAAGG AACAATTACC CGACCTTATC ATTGTAGACG
     3361 GCGGGATCAT CCAGATCCAC GCAGCGAAGG AGGTGTTGAA TAAGTTAAAT CTGAAGATTG
     3421 ATGTCATCGG ATTATCGAAA GATGACCATC ATAAAACCCG TTATTTGATC GACATTTTTG
     3481 AGCAGACGAT CGACATTAAG AATTTTAAGA AATTGTACAA TTTTTTGACG TCACTGCAAA
     3541 TCCGCGTAGA CGAATACGCT AAGTCGGGCT TCCGCAAGAA ATATCATAAC CAATTGAACG
     3601 ATCAGATTCT TTTAATCAAA GGTGTTGGCA AGAAAACTAA CTTGAAACTG TACAAGCATT
     3661 TTAAGACTAT TGACAATATC AAAAACGCTA GTTTCGACGA GTTGAACAAA GTGATTAATA
     3721 ACAAAAAGAT CACTAATTTA ATTATCTCGA ACTTAAACAA GTGAAGAGCT TAGAGACC
//