LOCUS       Exported     3809 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3809
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1822..1869
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3697..3713
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3714..3745
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0257"
                     /note="RnjB: Ribonuclease J2"
                     /translation="MPDIKFTALGGQDERGKNLYVLEIDNDFFILDAGVKYPEKDILGIDTVIPKFDYIKQNKKKIKGIFLTNPSAYNMGAVPYLLKEIEAPIYCNEITELIAKIKFQKLRLKNKDHILKVVHDKDILKIGNTKVEIFRTTSSSPQSFGYVFHTELGSIVYAGDYIIDGKEQSYFSTDYTHLSQIAKNGVLALISDAEFASRKDFTVPNHKIDKYILAPFKEKKTKIIVAIFEEDIHKLSQICMAAKENNRKIAVYGRTMDAVLRSNLIHENLVIKPEDLMSIQEYVNSDNGVLIISGTGDDLYSKLAKIATSNDSLVEFTEKDLIILTNTPVAGVEKRHAQILDELARTDARLLALSDKNIWSMRASYEDIKMLTSILNPKYFIPVKALYKDFLNAEKAAIEAGVNNQNIGIIDNGEILKLSKNHLAISEHSAEHGNVYVDGSGIGDVGSIVLNERKQLATDGVIIVGATIDSRNKELISLIDTQMRGVLYIQEDNPIFKILQREITNLILEGQALYKKEPKKYDLNEIKKDITSKIKQLIKQESGKTPIVLVIINESDGKAYVPRNNKKRYNNNNKSTKSKKDKS"
     primer_bind     complement(1874..1890)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1957..2545)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2716..3576)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(3577..3681)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /note="RnjB: Ribonuclease J2"
                     /translation="MPDIKFTALGGQDERGKNLYVLEIDNDFFILDAGVKYPEKDILGIDTVIPKFDYIKQNKKKIKGIFLTNPSAYNMGAVPYLLKEIEAPIYCNEITELIAKIKFQKLRLKNKDHILKVVHDKDILKIGNTKVEIFRTTSSSPQSFGYVFHTELGSIVYAGDYIIDGKEQSYFSTDYTHLSQIAKNGVLALISDAEFASRKDFTVPNHKIDKYILAPFKEKKTKIIVAIFEEDIHKLSQICMAAKENNRKIAVYGRTMDAVLRSNLIHENLVIKPEDLMSIQEYVNSDNGVLIISGTGDDLYSKLAKIATSNDSLVEFTEKDLIILTNTPVAGVEKRHAQILDELARTDARLLALSDKNIWSMRASYEDIKMLTSILNPKYFIPVKALYKDFLNAEKAAIEAGVNNQNIGIIDNGEILKLSKNHLAISEHSAEHGNVYVDGSGIGDVGSIVLNERKQLATDGVIIVGATIDSRNKELISLIDTQMRGVLYIQEDNPIFKILQREITNLILEGQALYKKEPKKYDLNEIKKDITSKIKQLIKQESGKTPIVLVIINESDGKAYVPRNNKKRYNNNNKSTKSKKDKS"
                     /label="MMSYN1_0257"
ORIGIN
        1 ATGCCGGATA TTAAATTCAC AGCTCTGGGA GGCCAGGACG AACGTGGGAA GAATCTGTAT
       61 GTTCTGGAGA TTGATAACGA CTTCTTTATT TTGGACGCGG GCGTGAAATA TCCAGAAAAA
      121 GACATTTTGG GCATCGATAC AGTGATCCCC AAGTTCGATT ATATCAAGCA AAACAAAAAG
      181 AAAATCAAGG GAATCTTTTT AACTAATCCG TCTGCCTACA ATATGGGTGC CGTTCCATAC
      241 TTGCTGAAAG AAATCGAGGC ACCAATTTAT TGTAACGAAA TTACAGAGTT GATCGCGAAA
      301 ATTAAATTTC AGAAATTACG CTTAAAGAAT AAAGATCACA TCCTGAAAGT TGTGCACGAT
      361 AAGGACATTT TGAAAATCGG CAACACCAAA GTAGAAATTT TTCGTACGAC CAGCAGCTCG
      421 CCACAGTCCT TCGGCTACGT TTTCCATACT GAATTAGGCT CTATCGTTTA CGCAGGGGAC
      481 TATATCATTG ACGGAAAGGA GCAGTCCTAC TTTTCGACAG ACTACACGCA CTTGTCACAA
      541 ATTGCCAAGA ACGGGGTTTT AGCTTTAATT TCGGACGCGG AATTCGCAAG CCGCAAAGAT
      601 TTCACCGTAC CAAATCATAA GATTGACAAA TACATTCTGG CTCCATTCAA AGAGAAGAAA
      661 ACGAAGATCA TCGTCGCAAT TTTTGAGGAA GATATCCACA AACTGAGCCA AATTTGTATG
      721 GCTGCGAAGG AAAATAATCG CAAAATCGCG GTCTATGGCC GTACAATGGA TGCGGTACTG
      781 CGTAGCAATC TTATCCACGA GAACCTTGTT ATTAAGCCCG AGGACTTAAT GTCTATTCAA
      841 GAATACGTCA ATTCAGATAA CGGGGTTTTA ATCATCTCGG GAACAGGGGA CGATTTATAC
      901 AGCAAATTAG CCAAGATCGC CACAAGCAAC GACTCACTTG TTGAGTTCAC TGAGAAAGAC
      961 TTAATCATCT TGACGAATAC GCCTGTTGCT GGGGTAGAAA AACGCCACGC ACAGATCTTA
     1021 GACGAGCTTG CTCGCACTGA CGCTCGGCTT CTGGCTTTGA GTGATAAAAA TATCTGGTCG
     1081 ATGCGTGCAA GCTACGAGGA TATCAAGATG TTAACGTCCA TCCTGAATCC CAAGTATTTC
     1141 ATCCCTGTCA AAGCTCTGTA TAAGGATTTT TTAAATGCGG AGAAGGCAGC TATCGAAGCC
     1201 GGTGTGAACA ATCAAAATAT CGGGATCATT GACAATGGAG AAATTCTGAA ACTGAGCAAG
     1261 AATCACTTAG CAATCTCGGA ACATAGCGCC GAGCACGGAA ACGTTTACGT GGACGGGTCG
     1321 GGCATTGGCG ACGTCGGATC AATTGTTTTG AACGAGCGTA AGCAGCTTGC AACGGATGGT
     1381 GTTATCATCG TGGGTGCAAC CATTGACTCA CGTAACAAAG AGTTAATTAG TTTGATTGAC
     1441 ACACAGATGC GCGGCGTGCT GTATATTCAA GAGGACAATC CTATCTTCAA GATCTTGCAG
     1501 CGTGAAATTA CAAATCTTAT TCTTGAAGGG CAGGCGTTAT ATAAAAAAGA ACCTAAAAAA
     1561 TATGACTTGA ATGAAATTAA GAAGGACATC ACGAGTAAAA TTAAACAATT GATTAAGCAG
     1621 GAGTCGGGGA AAACGCCGAT CGTCCTTGTA ATTATTAATG AAAGTGACGG AAAGGCATAC
     1681 GTTCCACGCA ACAATAAGAA ACGCTACAAT AACAACAATA AAAGTACGAA ATCGAAGAAA
     1741 GACAAGTCCT GAAGAGCTTA GAGACCGGTA CTAGTTCCTG CAGGGCTCGA GGTGTCAATC
     1801 GTCGGAGCCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG
     1861 GGTTTTTTGC ATGGTCATAG CTGTTTCCTG AGAGCTTGGC AGGTGATGAC ACACATTAAC
     1921 AAATTTCGTG AGGAGTCTCC AGAAGAATGC CATTAATTTC CATAGGCTCC GCCCCCCTGA
     1981 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG
     2041 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT
     2101 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG
     2161 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC
     2221 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT
     2281 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA
     2341 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC
     2401 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC
     2461 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT
     2521 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC
     2581 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT
     2641 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA
     2701 AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT
     2761 ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG
     2821 CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAA CCACGCTCAC CGGCTCCAGA
     2881 TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT
     2941 ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT
     3001 TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT
     3061 TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT
     3121 GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC
     3181 CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC
     3241 CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT
     3301 GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG
     3361 AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT
     3421 ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC
     3481 TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA
     3541 GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG
     3601 AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA
     3661 TAAACAAATA GGGGTTCCGC GCACCTGCAC CAGTCAGTAA AACGACGGCC AGTAGTCAAA
     3721 AGCCTCCGAC CGGAGGCTTT TGACTTGGTC AGGTGGAGTG AATTCGCGGC CGCTTCTAGA
     3781 GGCTCTTCGT AATTACGAAG AGGTCTCTA
//