LOCUS       Exported     2855 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2855
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      868..915
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2743..2759
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2760..2791
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             1..0
                     /label="MMSYN1_0259"
                     /note="NadK: NAD kinase"
                     /translation="MKYSFITNKYEESSDIVDELLNILKNTDFKKDQNNPDICFVIGGDGTFLYAVHKYQSILDKLIFIPIKFGGIGFYTNKNRVDDLKKIDLNKIIEQPNITELGLIEVNYDDQKVYAINEIKITNQVRPLNLDIYINNEFLEQFKGTGLVFSTPSGSTGFMKSANGAIIYPVVSLFEMQELMPISTNKFRTLNAPIIFSDNEHITLKLEDLNNVTLSADTYEYQFKNKELLIKLSRKKIKLLNLNKDKFNKIKILRDIFVLNDKTKN"
     primer_bind     complement(920..936)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(1003..1591)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(1762..2622)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(2623..2727)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /translation="MKYSFITNKYEESSDIVDELLNILKNTDFKKDQNNPDICFVIGGDGTFLYAVHKYQSILDKLIFIPIKFGGIGFYTNKNRVDDLKKIDLNKIIEQPNITELGLIEVNYDDQKVYAINEIKITNQVRPLNLDIYINNEFLEQFKGTGLVFSTPSGSTGFMKSANGAIIYPVVSLFEMQELMPISTNKFRTLNAPIIFSDNEHITLKLEDLNNVTLSADTYEYQFKNKELLIKLSRKKIKLLNLNKDKFNKIKILRDIFVLNDKTKN"
                     /label="MMSYN1_0259"
                     /note="NadK: NAD kinase"
ORIGIN
        1 ATGAAGTACT CTTTCATTAC CAACAAATAC GAAGAATCGA GCGATATTGT GGATGAGCTG
       61 CTGAATATTC TGAAAAACAC CGACTTTAAG AAGGACCAAA ACAATCCCGA TATTTGTTTT
      121 GTAATTGGCG GCGACGGAAC CTTCCTTTAT GCTGTTCATA AGTATCAATC GATCTTGGAC
      181 AAACTGATCT TCATTCCAAT TAAGTTTGGT GGAATCGGGT TTTATACAAA TAAGAATCGC
      241 GTGGATGACT TGAAGAAAAT CGATTTAAAT AAGATCATCG AGCAGCCAAA CATCACGGAG
      301 TTAGGTTTAA TCGAGGTGAA TTACGACGAT CAGAAAGTGT ATGCCATCAA CGAAATCAAG
      361 ATCACGAACC AGGTACGCCC TCTTAACTTG GACATTTATA TCAACAATGA GTTTCTTGAG
      421 CAATTCAAAG GCACAGGCTT AGTTTTCAGC ACGCCGTCTG GAAGCACAGG GTTTATGAAG
      481 TCTGCAAACG GGGCAATCAT CTACCCGGTC GTGTCCTTAT TTGAAATGCA GGAATTGATG
      541 CCCATCTCGA CCAACAAGTT TCGTACTTTA AATGCACCAA TTATCTTCTC AGATAACGAG
      601 CATATCACGC TTAAGTTAGA GGATTTAAAC AATGTTACGC TGAGTGCTGA TACCTACGAA
      661 TACCAGTTTA AGAATAAAGA ACTTTTGATT AAGTTGAGCC GTAAGAAAAT CAAACTGCTT
      721 AATCTGAATA AGGACAAATT TAACAAGATC AAAATTTTGC GCGATATTTT CGTCTTAAAC
      781 GATAAGACTA AGAACTGAAG AGCTTAGAGA CCGGTACTAG TTCCTGCAGG GCTCGAGGTG
      841 TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC
      901 TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT GATGACACAC
      961 ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA GGCTCCGCCC
     1021 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT
     1081 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT
     1141 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG
     1201 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA
     1261 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA
     1321 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC
     1381 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG
     1441 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG
     1501 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA
     1561 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC
     1621 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG
     1681 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA
     1741 TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT
     1801 CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG
     1861 GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGAACCAC GCTCACCGGC
     1921 TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC
     1981 AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC
     2041 GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC
     2101 GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC
     2161 CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA
     2221 GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT
     2281 GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA
     2341 GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA
     2401 TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG
     2461 GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC
     2521 AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC
     2581 AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA
     2641 TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA
     2701 GAAAAATAAA CAAATAGGGG TTCCGCGCAC CTGCACCAGT CAGTAAAACG ACGGCCAGTA
     2761 GTCAAAAGCC TCCGACCGGA GGCTTTTGAC TTGGTCAGGT GGAGTGAATT CGCGGCCGCT
     2821 TCTAGAGGCT CTTCGTAATT ACGAAGAGGT CTCTA
//