LOCUS Exported 4642 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..4642 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 38..85 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 1933..1949 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 1950..1981 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 2010..4628 /label="MMSYN1_0260" /note="Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a 'posttransfer' editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner." /translation="MKKQLNPKYDHLQVEKDKYQYWLDKNLFKADINSNKPKFSIILPPPNVTGKLHIGHAWDTSLQDAIIRFKKLTGYDTLFLPGMDHSGISTQVKVEAKLKQQGIDKNKLGREKFLLEAWKWKEEYANIIRKQWAKLGLSLDYSTEKFTLDEDINQIVKEIFVKFYNDQIIYKDKQIVNWDPEQKTAISNVEVIYKETQSKMYYFKYMLEDSNEYLTVATTRPETMFVDQCLVVNPNDSRYQKYINKKVINPVNKQVIPIISDEYVDIEFGTGVMKCTPAHDLNDYHLAIKHNLKMPICLNIDGSVNQLGGKYQGLDRFVARKEIIKNAINEDLFVKEEDIINQVGFSERSNAIVEPYLSDQWFVKMDKFKNMVIDLQNSDNKINFYPNRFSDVLNRWMTNAHDWCISRQLWWGHQIPCWYHKKTNEMYVGINPPSDIENWTQDQDVLDTWFSSGLWAFSTLLNNKGLESEYFKNYFPTSVLVTGYDIIFFWVARMIFQTLEYTKQIPFKDVLIHGLVRDELNRKMSKSLGNGIDPMDVINNNGCDSLRLFLLTNSTPGQDIRYSNEKILASSNFINKLWNASRYVFLNLDKDFKFDQNFYKTDLEITNQWILTELSKTQAYVYEKMNKYEFSLAGNHLWDFVWNKYCSWYIEFSKVNLNNDKFNHQTKQTLFYVLKEILIMIHPLIPFVSEEIYLNMMLKESILLEQWTNLNSNYDTSFIDDVIKMITSIREFKNTKNIKNDVCLSVNVSNTNQNHTKLFKKHFDQIYSFLINFCNTKLVDYVIKNKTSLSIDEYFIEIANDSFINKNELIKELEQKQNHLNNEITRSQKILNNQEFIKKAKPEKIQSEKIKYQNYLDQLQAIKDKLKELKND" primer_bind complement(90..106) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(173..761) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(952..1812) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(1813..1917) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC 61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT 121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA 181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG 721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA 781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG 961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG 1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC 1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC 1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA 1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC 1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG 1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC 1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT 1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG 1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC 1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG 1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT 1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG 1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG 1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC 1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGAAAAAGCA GTTGAATCCC AAGTACGATC 2041 ATTTACAAGT AGAAAAGGAC AAATACCAAT ATTGGTTAGA CAAGAACCTG TTCAAGGCCG 2101 ATATTAACAG CAATAAACCA AAGTTCTCCA TTATCTTGCC CCCTCCAAAT GTAACCGGGA 2161 AGCTGCATAT CGGCCATGCG TGGGACACCT CTCTGCAAGA TGCGATCATC CGCTTCAAAA 2221 AATTAACGGG GTATGATACG TTGTTTCTGC CAGGCATGGA CCATTCCGGT ATCAGCACTC 2281 AGGTGAAAGT AGAAGCTAAG TTAAAGCAAC AAGGCATCGA TAAGAATAAG CTGGGCCGTG 2341 AAAAATTTTT ATTAGAGGCC TGGAAGTGGA AGGAAGAATA TGCAAACATT ATTCGTAAAC 2401 AATGGGCTAA ATTAGGTCTG AGTCTGGACT ATTCGACTGA GAAGTTCACA TTGGATGAGG 2461 ATATCAACCA GATCGTCAAG GAGATTTTTG TTAAATTTTA CAATGATCAA ATCATCTACA 2521 AAGACAAACA GATTGTTAAT TGGGACCCCG AACAAAAAAC CGCTATTTCA AACGTCGAGG 2581 TCATTTATAA GGAAACACAA TCTAAGATGT ATTATTTCAA ATATATGTTA GAAGATTCAA 2641 ACGAATACTT AACCGTCGCG ACGACACGTC CAGAAACCAT GTTTGTGGAT CAGTGTTTAG 2701 TCGTAAACCC GAATGATTCC CGTTACCAGA AGTATATCAA CAAGAAAGTT ATCAATCCAG 2761 TCAACAAACA AGTAATCCCG ATTATCTCTG ATGAATATGT AGACATTGAG TTCGGGACCG 2821 GGGTCATGAA ATGCACTCCT GCTCACGACT TGAATGACTA TCACTTAGCT ATTAAACACA 2881 ATTTAAAGAT GCCGATCTGT CTTAACATTG ACGGAAGCGT TAATCAATTG GGAGGGAAAT 2941 ATCAGGGGCT GGACCGTTTT GTGGCGCGCA AGGAGATTAT CAAAAACGCT ATCAACGAGG 3001 ACTTGTTCGT TAAAGAGGAG GATATTATCA ATCAGGTTGG GTTCTCTGAA CGTAGCAACG 3061 CGATCGTAGA GCCATACCTG TCGGATCAGT GGTTCGTGAA AATGGACAAG TTCAAGAATA 3121 TGGTGATCGA TCTTCAGAAC AGTGATAACA AGATTAATTT CTATCCGAAT CGTTTCTCAG 3181 ACGTTCTGAA CCGTTGGATG ACCAACGCTC ATGACTGGTG CATTAGCCGT CAGCTGTGGT 3241 GGGGACATCA AATTCCATGT TGGTATCATA AGAAAACCAA CGAGATGTAT GTCGGTATCA 3301 ACCCGCCGTC GGATATTGAA AACTGGACCC AGGATCAGGA TGTACTGGAT ACCTGGTTTT 3361 CCTCTGGCCT GTGGGCTTTC TCTACTTTGC TGAACAACAA AGGGTTGGAG TCCGAGTATT 3421 TTAAGAATTA CTTCCCAACA TCTGTTCTTG TCACAGGGTA CGACATCATC TTCTTTTGGG 3481 TGGCTCGTAT GATTTTTCAG ACCTTAGAAT ACACGAAGCA GATTCCTTTC AAAGATGTTC 3541 TGATTCATGG GCTTGTTCGT GATGAACTGA ATCGTAAGAT GAGCAAATCT CTTGGGAACG 3601 GTATCGATCC TATGGATGTG ATTAATAATA ATGGATGTGA CAGCCTGCGC CTGTTTCTTT 3661 TAACCAATAG CACGCCGGGA CAAGATATTC GTTATTCGAA TGAAAAAATC CTTGCAAGTT 3721 CGAATTTCAT CAACAAACTT TGGAACGCTT CTCGTTACGT GTTTTTAAAC TTAGATAAAG 3781 ACTTCAAATT TGATCAAAAC TTCTACAAGA CGGACTTAGA GATCACTAAC CAGTGGATTT 3841 TAACTGAATT ATCCAAGACG CAGGCTTACG TGTATGAGAA GATGAATAAG TATGAGTTCA 3901 GTCTTGCGGG CAACCATTTA TGGGACTTCG TATGGAATAA GTACTGTAGC TGGTATATTG 3961 AATTTAGCAA AGTCAACTTG AATAATGATA AATTTAATCA CCAAACGAAG CAGACCCTGT 4021 TTTATGTCCT GAAGGAAATC CTGATCATGA TTCATCCGCT GATTCCGTTC GTGAGTGAAG 4081 AGATCTACTT GAACATGATG TTGAAGGAAT CAATCTTGCT GGAGCAGTGG ACAAACTTAA 4141 ACAGTAACTA TGACACCTCG TTTATCGACG ATGTTATCAA GATGATCACG AGCATCCGCG 4201 AGTTCAAAAA CACTAAAAAC ATTAAAAACG ATGTGTGTCT GTCCGTAAAC GTGTCCAACA 4261 CCAATCAGAA CCATACCAAA CTTTTTAAAA AGCACTTTGA CCAAATTTAC TCCTTCCTGA 4321 TCAACTTTTG TAACACGAAA TTAGTAGACT ATGTGATTAA AAACAAGACA AGTCTGTCTA 4381 TTGACGAGTA TTTTATCGAG ATTGCCAACG ATTCATTTAT CAATAAGAAT GAATTAATCA 4441 AGGAGCTTGA GCAAAAGCAA AATCACCTTA ACAATGAGAT TACCCGTTCT CAGAAGATTT 4501 TAAACAACCA GGAATTCATC AAAAAAGCGA AACCAGAAAA GATTCAGTCC GAGAAAATCA 4561 AATATCAGAA CTACTTGGAC CAGCTTCAGG CGATCAAGGA TAAATTAAAG GAATTAAAGA 4621 ATGATTGAAG AGCTTAGAGA CC //