LOCUS       Exported     2735 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2735
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      748..795
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2623..2639
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2640..2671
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0262"
                     /note=""
                     /translation="MKKYIIAPSVLSANFMELKNELELCKKNNINWIHYDVMDFDFVPNLTFGSKILHDIKKNIDINVDVHFMVSVKTKQFEDFFSDYIKAKPEMMTMHIESLKDDNTINKFIDLCKQNNILASLAISPKTDVSLVYPYLDKLDNVLVMSVEPGFGGQKFISSSLEKIQILDQLRNTKNYKYTIEVDGGINEQTSVLVKQAGVDMIVAGSYLFGSDDFTKRAKGLFDEL"
     primer_bind     complement(800..816)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(883..1471)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1642..2502)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(2503..2607)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0262"
                     /note=""
                     /translation="MKKYIIAPSVLSANFMELKNELELCKKNNINWIHYDVMDFDFVPNLTFGSKILHDIKKNIDINVDVHFMVSVKTKQFEDFFSDYIKAKPEMMTMHIESLKDDNTINKFIDLCKQNNILASLAISPKTDVSLVYPYLDKLDNVLVMSVEPGFGGQKFISSSLEKIQILDQLRNTKNYKYTIEVDGGINEQTSVLVKQAGVDMIVAGSYLFGSDDFTKRAKGLFDEL"
ORIGIN
        1 ATGAAAAAAT ACATTATCGC GCCTTCAGTG CTTTCAGCGA ATTTCATGGA GTTAAAAAAT
       61 GAGTTGGAAT TATGCAAGAA GAATAATATT AACTGGATCC ATTATGACGT GATGGACTTC
      121 GATTTTGTTC CTAATCTTAC TTTTGGCAGT AAGATCTTGC ATGATATCAA GAAGAATATT
      181 GACATTAATG TTGATGTTCA CTTCATGGTA TCAGTAAAGA CCAAGCAATT TGAGGACTTC
      241 TTTTCAGACT ATATTAAAGC GAAACCCGAA ATGATGACCA TGCACATCGA GTCTTTAAAA
      301 GATGACAATA CCATTAATAA GTTTATCGAC TTATGTAAGC AAAATAATAT CTTAGCGAGT
      361 TTAGCCATTT CCCCAAAAAC TGATGTGTCC CTGGTCTACC CATATTTAGA TAAGCTGGAT
      421 AACGTCCTTG TTATGAGTGT CGAGCCAGGT TTCGGTGGAC AAAAGTTCAT CAGCTCAAGC
      481 CTGGAGAAAA TCCAAATCCT GGATCAATTA CGCAACACCA AGAACTACAA GTACACAATT
      541 GAAGTGGACG GAGGCATCAA TGAGCAAACG TCCGTTCTGG TGAAACAAGC GGGCGTCGAT
      601 ATGATTGTGG CCGGCAGTTA TCTTTTCGGC AGCGACGATT TTACTAAACG TGCAAAGGGG
      661 CTTTTTGATG AACTGTGAAG AGCTTAGAGA CCGGTACTAG TTCCTGCAGG GCTCGAGGTG
      721 TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC
      781 TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT GATGACACAC
      841 ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA GGCTCCGCCC
      901 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT
      961 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT
     1021 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG
     1081 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA
     1141 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA
     1201 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC
     1261 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG
     1321 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG
     1381 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA
     1441 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC
     1501 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG
     1561 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA
     1621 TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT
     1681 CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG
     1741 GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGAACCAC GCTCACCGGC
     1801 TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC
     1861 AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC
     1921 GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC
     1981 GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC
     2041 CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA
     2101 GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT
     2161 GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA
     2221 GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA
     2281 TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG
     2341 GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC
     2401 AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC
     2461 AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA
     2521 TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA
     2581 GAAAAATAAA CAAATAGGGG TTCCGCGCAC CTGCACCAGT CAGTAAAACG ACGGCCAGTA
     2641 GTCAAAAGCC TCCGACCGGA GGCTTTTGAC TTGGTCAGGT GGAGTGAATT CGCGGCCGCT
     2701 TCTAGAGGCT CTTCGTAATT ACGAAGAGGT CTCTA
//