LOCUS       Exported     2960 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2960
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      973..1020
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2848..2864
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2865..2896
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0263"
                     /note="One of several proteins that assist in the late maturation steps of the functional core of the 30S ribosomal subunit. Helps release RbfA from mature subunits. May play a role in the assembly of ribosomal proteins into the subunit. Circularly permuted GTPase that catalyzes slow GTP hydrolysis, GTPase activity is stimulated by the 30S ribosomal subunit."
                     /translation="MQGILIKKDSNESYVFYKNNIYKVYAKGNLKKELKPKVGDIVEFSILEDGNGYIKEIKPRKNSLDRPKIANVDQVLIVTALYEPLFSSFLLNKYIMMLETLKIKPILIFTKVDLLKQTPYYQEVMHKINWYEKMHYEVLIINNKDNLENKKAIIEKLKSFLENKISVFTGQTGAGKSTTLNNFLDMNNQIKTNEISKKLNRGKHTTTSIQLYNLENNIFIADTPGFSAFDLNNIDIEHILYSWETFIPFLNKCKFVDCTHTHESKCEVKKAVAEHLLPTFIYNDYVKVYEELKQNRKNKY"
     primer_bind     complement(1025..1041)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(1108..1696)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1867..2727)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2728..2832)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0263"
                     /note="One of several proteins that assist in the late maturation steps of the functional core of the 30S ribosomal subunit. Helps release RbfA from mature subunits. May play a role in the assembly of ribosomal proteins into the subunit. Circularly permuted GTPase that catalyzes slow GTP hydrolysis, GTPase activity is stimulated by the 30S ribosomal subunit."
                     /translation="MQGILIKKDSNESYVFYKNNIYKVYAKGNLKKELKPKVGDIVEFSILEDGNGYIKEIKPRKNSLDRPKIANVDQVLIVTALYEPLFSSFLLNKYIMMLETLKIKPILIFTKVDLLKQTPYYQEVMHKINWYEKMHYEVLIINNKDNLENKKAIIEKLKSFLENKISVFTGQTGAGKSTTLNNFLDMNNQIKTNEISKKLNRGKHTTTSIQLYNLENNIFIADTPGFSAFDLNNIDIEHILYSWETFIPFLNKCKFVDCTHTHESKCEVKKAVAEHLLPTFIYNDYVKVYEELKQNRKNKY"
ORIGIN
        1 ATGCAGGGCA TCCTTATTAA GAAAGATAGC AACGAATCTT ACGTATTCTA CAAGAACAAT
       61 ATCTACAAAG TGTATGCCAA GGGAAATTTA AAAAAGGAGT TGAAACCAAA GGTTGGCGAC
      121 ATCGTAGAAT TTTCTATTCT TGAGGATGGT AATGGGTACA TCAAAGAAAT CAAACCTCGT
      181 AAAAACTCCC TTGACCGCCC CAAGATCGCG AATGTGGACC AGGTGTTGAT TGTAACGGCG
      241 TTATATGAAC CGTTATTCTC GTCCTTCTTG CTTAATAAGT ACATTATGAT GTTAGAAACG
      301 TTAAAGATCA AACCCATCCT GATTTTCACG AAGGTAGACT TACTGAAACA AACTCCTTAT
      361 TATCAAGAAG TGATGCATAA AATTAATTGG TACGAGAAAA TGCACTACGA AGTGTTAATC
      421 ATTAATAACA AAGACAACTT GGAGAACAAG AAAGCCATCA TCGAAAAACT CAAGAGCTTC
      481 TTGGAAAACA AAATCAGCGT CTTTACTGGA CAGACAGGTG CAGGGAAATC CACCACGCTT
      541 AACAATTTCC TTGATATGAA TAATCAGATT AAAACTAACG AGATTTCGAA GAAATTAAAT
      601 CGTGGAAAGC ACACTACTAC CTCGATCCAG TTGTATAACC TGGAAAATAA CATTTTCATT
      661 GCGGATACAC CAGGATTCTC CGCATTCGAT TTGAATAATA TCGATATCGA GCATATTTTG
      721 TATTCTTGGG AAACTTTCAT TCCCTTTTTA AACAAATGCA AATTTGTCGA CTGCACCCAT
      781 ACTCATGAGT CAAAATGTGA AGTCAAAAAG GCGGTGGCCG AACACTTGTT ACCTACATTC
      841 ATTTATAACG ATTATGTCAA GGTCTATGAG GAGTTAAAAC AGAATCGTAA GAACAAGTAC
      901 TGAAGAGCTT AGAGACCGGT ACTAGTTCCT GCAGGGCTCG AGGTGTCAAT CGTCGGAGCC
      961 GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG
     1021 CATGGTCATA GCTGTTTCCT GAGAGCTTGG CAGGTGATGA CACACATTAA CAAATTTCGT
     1081 GAGGAGTCTC CAGAAGAATG CCATTAATTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA
     1141 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC
     1201 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA
     1261 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA
     1321 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA
     1381 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA
     1441 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG
     1501 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG
     1561 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG
     1621 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG
     1681 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA
     1741 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT
     1801 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC
     1861 TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC
     1921 ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC
     1981 TGGCCCCAGT GCTGCAATGA TACCGCGAGA ACCACGCTCA CCGGCTCCAG ATTTATCAGC
     2041 AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC
     2101 CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT
     2161 GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC
     2221 TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA
     2281 AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT
     2341 ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG
     2401 CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC
     2461 GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA
     2521 AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT
     2581 GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT
     2641 CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG
     2701 GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA
     2761 TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT
     2821 AGGGGTTCCG CGCACCTGCA CCAGTCAGTA AAACGACGGC CAGTAGTCAA AAGCCTCCGA
     2881 CCGGAGGCTT TTGACTTGGT CAGGTGGAGT GAATTCGCGG CCGCTTCTAG AGGCTCTTCG
     2941 TAATTACGAA GAGGTCTCTA
//