LOCUS Exported 3448 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3448 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 38..85 /note="transcription terminator for bacteriophage T7 RNA polymerase" /label="T7 terminator" primer_bind 1933..1949 /note="common sequencing primer, one of multiple similar variants" /label="M13 for" terminator 1950..1981 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 2010..3434 /label="MMSYN1_0282" /note="ProRS: Prolyl-tRNA synthetase" /translation="MKKQLDKITPRNIDFSQWYTDIVLNTKLASYGPVKGTMIFRPYGYRIWELIQKYLDEEFKKINVDNVYFPLLIPESLFNKEKDHIEGFSPEIATVTKVGEKQLEENLFIRPTSEVVMMDYFSNEINSYRDLPLIYNQWCNVMRWEKTTRPFLRTSEFLWQEGHTVHSSYNEAEDFCLKILNIYEKFAKDILLLPVICGKKTEKEKFAGAKDTYTIESLMFDGQALQCGTSHFFADNFTKVYDIKFQNKENKLEHAYSTSWGVSTRLIGALIMTHSDDNGLVLPSKISPIQIQIIQIKNTEQIDQVVEIIKDKLSDYRIDVDNSDKSFGFKISEAEIKGIPIRIEIGPRDLENNQITISRRDQQENKIKIDYKDVKKVVDQMIKDYDLALYNNALENRKNRTFKADTIEEYIEILKQNQGFVLVPFCGRVECEQDIKTKTATNSRCIPFDQKEVKAKCFNCKKDTCLQVIFARAY" primer_bind complement(90..106) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(173..761) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(952..1812) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(1813..1917) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC 61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT 121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA 181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG 721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA 781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG 961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG 1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC 1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC 1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA 1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC 1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG 1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC 1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT 1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG 1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC 1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG 1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT 1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG 1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG 1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC 1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGAAGAAACA ATTAGACAAA ATCACGCCTC 2041 GCAATATTGA TTTCAGCCAG TGGTACACTG ACATCGTTCT GAACACCAAA CTGGCATCCT 2101 ATGGCCCCGT CAAAGGGACG ATGATTTTCC GCCCGTACGG TTACCGCATC TGGGAATTGA 2161 TCCAGAAATA TCTTGATGAG GAATTCAAGA AAATCAACGT CGATAATGTA TACTTTCCAT 2221 TGCTTATTCC CGAAAGTCTG TTCAACAAGG AAAAAGACCA TATTGAAGGG TTCTCTCCGG 2281 AAATTGCTAC TGTTACCAAA GTGGGCGAAA AACAACTTGA GGAAAATCTT TTTATCCGCC 2341 CGACTAGCGA AGTAGTGATG ATGGATTATT TCAGCAACGA GATTAACTCC TACCGCGATT 2401 TACCGCTTAT TTATAACCAA TGGTGCAATG TGATGCGCTG GGAAAAGACC ACTCGTCCTT 2461 TTCTGCGTAC ATCGGAATTC CTTTGGCAAG AGGGTCACAC GGTACACTCT TCCTACAATG 2521 AAGCAGAGGA CTTTTGCCTG AAGATCCTTA ACATTTACGA GAAATTTGCC AAGGACATCT 2581 TGTTGTTACC TGTAATCTGT GGAAAGAAAA CAGAAAAGGA AAAGTTCGCC GGCGCTAAAG 2641 ATACCTATAC GATTGAAAGT CTTATGTTCG ACGGGCAAGC GCTTCAATGC GGTACATCAC 2701 ACTTTTTTGC TGACAACTTT ACTAAAGTTT ACGATATTAA GTTCCAGAAC AAAGAGAACA 2761 AATTGGAACA TGCCTATTCG ACATCGTGGG GTGTGTCGAC GCGCTTGATT GGTGCCTTAA 2821 TTATGACACA CAGTGACGAC AATGGCTTAG TCCTGCCTAG TAAAATCAGC CCGATCCAAA 2881 TCCAGATTAT TCAGATCAAG AATACGGAAC AGATTGACCA GGTTGTGGAG ATCATCAAGG 2941 ATAAACTTTC CGATTATCGT ATCGATGTCG ACAATTCTGA CAAGAGTTTC GGGTTCAAGA 3001 TCAGCGAAGC GGAGATTAAG GGGATTCCGA TTCGCATCGA GATCGGTCCA CGTGACTTGG 3061 AAAACAATCA AATTACGATT TCCCGTCGTG ACCAACAAGA GAATAAAATC AAGATCGACT 3121 ACAAGGACGT AAAGAAAGTT GTAGATCAGA TGATTAAAGA CTATGACTTA GCACTTTACA 3181 ACAATGCGTT GGAAAACCGT AAGAACCGCA CTTTCAAGGC AGACACTATC GAGGAATATA 3241 TTGAAATCTT AAAGCAAAAT CAAGGATTTG TACTGGTACC TTTCTGCGGA CGTGTCGAGT 3301 GTGAGCAAGA CATCAAAACC AAGACCGCAA CCAATTCACG TTGTATCCCC TTCGACCAAA 3361 AGGAGGTAAA AGCCAAATGT TTTAATTGTA AGAAGGATAC TTGTCTGCAA GTAATCTTTG 3421 CGCGTGCTTA CTGAAGAGCT TAGAGACC //