LOCUS       Exported     3860 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3860
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      1873..1920
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3748..3764
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3765..3796
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             1..0
                     /translation="MDKSKIRNFSIIAHIDHGKSTLADRILELTNTVEKREMQDQLLDSMDIERERGITIKLNSVQLKYHSKDNQDYIFNLIDTPGHVDFTYEVSRSLAACEGAILVVDASQGVEAQTLANVYLAIDSNLEIIPVINKIDLPSADVDKVKQEIEEIIGLDCSNAPLISAKTGLNVEDVLQAIVERIPSPSDAIDNAPLKALIFDSYYDKYLGVVMSIRLKQGMLRVGDKIKLMSTNAEYEVTYLGIKTPKIVKKDFLEAGEVGWVAASIKTIKDVNVGDTITSVLNPADEPLEGYKKLKPMVYCGIYPIDTNKYQDFKEALEKIELSDSSLVYEPETSQALGFGFRCGFLGLLHMEVIQERLEREYNLELIATAPSVVYKVHLTNKQIIELDNPALLPEAQKIAKIEEPFVEIKIATPSEYIGDLMNLCQNKLGIYKNMEVIDNNRRILIYQMPLAEIIFDFFNKLKSISKGYASFEYELIGYKESKLVRMDIKLNGEMVDAFSMIVNQKFAYQRGSALTLKLKELIPRQNFEVPVQATIGNKVISRETIKAYRKDVTWKLHAADKSRRKKLLEKQKEGKKKMKEIGTVEVPQEAFVAILKIDD"
                     /label="MMSYN1_0285"
                     /note="Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner."
     primer_bind     complement(1925..1941)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(2008..2596)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2767..3627)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3628..3732)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /translation="MDKSKIRNFSIIAHIDHGKSTLADRILELTNTVEKREMQDQLLDSMDIERERGITIKLNSVQLKYHSKDNQDYIFNLIDTPGHVDFTYEVSRSLAACEGAILVVDASQGVEAQTLANVYLAIDSNLEIIPVINKIDLPSADVDKVKQEIEEIIGLDCSNAPLISAKTGLNVEDVLQAIVERIPSPSDAIDNAPLKALIFDSYYDKYLGVVMSIRLKQGMLRVGDKIKLMSTNAEYEVTYLGIKTPKIVKKDFLEAGEVGWVAASIKTIKDVNVGDTITSVLNPADEPLEGYKKLKPMVYCGIYPIDTNKYQDFKEALEKIELSDSSLVYEPETSQALGFGFRCGFLGLLHMEVIQERLEREYNLELIATAPSVVYKVHLTNKQIIELDNPALLPEAQKIAKIEEPFVEIKIATPSEYIGDLMNLCQNKLGIYKNMEVIDNNRRILIYQMPLAEIIFDFFNKLKSISKGYASFEYELIGYKESKLVRMDIKLNGEMVDAFSMIVNQKFAYQRGSALTLKLKELIPRQNFEVPVQATIGNKVISRETIKAYRKDVTWKLHAADKSRRKKLLEKQKEGKKKMKEIGTVEVPQEAFVAILKIDD"
                     /label="MMSYN1_0285"
                     /note="Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner."
ORIGIN
        1 ATGGATAAGA GTAAAATTCG TAACTTTAGC ATTATTGCGC ATATTGACCA CGGTAAATCT
       61 ACGCTGGCGG ATCGTATTTT AGAGCTGACT AATACCGTTG AGAAACGTGA GATGCAAGAC
      121 CAACTGTTGG ACAGCATGGA CATTGAACGT GAACGTGGAA TTACTATTAA GTTGAATTCC
      181 GTGCAGTTAA AATATCATTC CAAAGATAAT CAAGACTACA TTTTTAACTT AATCGATACT
      241 CCTGGGCATG TCGACTTCAC GTACGAAGTG TCTCGCTCGT TGGCAGCCTG TGAAGGTGCT
      301 ATTTTAGTTG TAGACGCCAG CCAGGGCGTT GAGGCCCAGA CCTTGGCGAA CGTATACTTA
      361 GCAATCGATT CTAATTTGGA AATTATCCCC GTCATTAACA AAATTGACTT ACCTTCCGCC
      421 GACGTGGACA AGGTTAAACA AGAAATCGAG GAAATCATCG GCCTGGATTG CAGTAACGCG
      481 CCCCTTATCT CAGCTAAGAC CGGGCTTAAT GTCGAGGACG TTTTACAAGC GATCGTTGAA
      541 CGTATTCCTA GCCCAAGTGA TGCGATTGAC AATGCTCCGC TTAAAGCGTT AATTTTCGAC
      601 TCGTACTATG ATAAGTATCT GGGCGTCGTC ATGTCAATTC GTTTAAAGCA GGGAATGTTA
      661 CGCGTGGGGG ATAAAATCAA GCTGATGTCG ACCAATGCTG AGTATGAGGT AACCTATCTG
      721 GGTATTAAAA CGCCAAAAAT CGTTAAAAAG GACTTCTTGG AGGCGGGCGA AGTTGGCTGG
      781 GTAGCGGCGT CGATCAAAAC GATCAAGGAC GTGAACGTTG GAGACACGAT CACGTCAGTC
      841 TTAAACCCAG CCGACGAACC TTTAGAAGGC TACAAGAAAT TAAAACCTAT GGTGTACTGC
      901 GGGATTTACC CCATCGATAC TAACAAGTAC CAGGACTTCA AGGAGGCATT AGAGAAGATT
      961 GAACTGAGCG ACAGCAGTCT GGTATATGAA CCTGAGACTT CGCAAGCCTT GGGGTTCGGT
     1021 TTCCGCTGTG GATTCCTTGG TTTGTTGCAC ATGGAAGTTA TCCAGGAGCG TCTTGAGCGT
     1081 GAATACAATC TGGAGCTTAT TGCTACAGCC CCGAGTGTAG TTTACAAGGT TCACCTGACC
     1141 AACAAGCAGA TTATTGAACT GGATAACCCA GCCTTGCTGC CCGAAGCGCA GAAGATCGCA
     1201 AAGATCGAGG AACCATTTGT TGAAATCAAA ATCGCCACAC CATCTGAGTA TATCGGGGAT
     1261 CTTATGAATC TGTGCCAGAA CAAGCTGGGA ATCTACAAGA ACATGGAGGT AATCGACAAT
     1321 AATCGTCGCA TCCTGATCTA CCAAATGCCA CTTGCAGAGA TTATCTTCGA CTTCTTTAAC
     1381 AAATTAAAGT CGATCTCTAA GGGATATGCG AGTTTTGAGT ACGAGCTTAT CGGGTACAAA
     1441 GAGTCTAAGT TGGTCCGCAT GGATATTAAA CTTAACGGAG AAATGGTTGA TGCTTTTTCA
     1501 ATGATTGTTA ACCAGAAATT CGCATACCAG CGCGGGAGTG CCTTAACGTT GAAATTGAAA
     1561 GAGTTGATTC CCCGCCAGAA TTTCGAAGTT CCAGTACAAG CCACAATCGG TAATAAGGTT
     1621 ATTTCACGCG AAACGATCAA GGCCTACCGC AAGGACGTTA CATGGAAACT TCACGCTGCT
     1681 GACAAAAGTC GCCGTAAGAA ACTGTTAGAG AAACAGAAGG AAGGGAAGAA GAAAATGAAA
     1741 GAAATCGGAA CAGTCGAGGT TCCCCAAGAG GCTTTCGTCG CAATCTTAAA GATCGACGAT
     1801 TGAAGAGCTT AGAGACCGGT ACTAGTTCCT GCAGGGCTCG AGGTGTCAAT CGTCGGAGCC
     1861 GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG
     1921 CATGGTCATA GCTGTTTCCT GAGAGCTTGG CAGGTGATGA CACACATTAA CAAATTTCGT
     1981 GAGGAGTCTC CAGAAGAATG CCATTAATTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA
     2041 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC
     2101 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA
     2161 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA
     2221 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA
     2281 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA
     2341 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG
     2401 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG
     2461 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG
     2521 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG
     2581 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA
     2641 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT
     2701 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC
     2761 TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC
     2821 ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC
     2881 TGGCCCCAGT GCTGCAATGA TACCGCGAGA ACCACGCTCA CCGGCTCCAG ATTTATCAGC
     2941 AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC
     3001 CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT
     3061 GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC
     3121 TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA
     3181 AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT
     3241 ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG
     3301 CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC
     3361 GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA
     3421 AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT
     3481 GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT
     3541 CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG
     3601 GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA
     3661 TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT
     3721 AGGGGTTCCG CGCACCTGCA CCAGTCAGTA AAACGACGGC CAGTAGTCAA AAGCCTCCGA
     3781 CCGGAGGCTT TTGACTTGGT CAGGTGGAGT GAATTCGCGG CCGCTTCTAG AGGCTCTTCG
     3841 TAATTACGAA GAGGTCTCTA
//