LOCUS       Exported     3782 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3782
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1795..1842
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3670..3686
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3687..3718
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0287"
                     /note="AspRS: Aspartyl-tRNA synthetase"
                     /translation="MKRTHTCGELTIDNIDQEVILQGWVKKIRKLGAMVFIDLKDRYGITQLVIEQENIDLINNVKNEYVIEIKGNVVKRKSINKELVTGDIEVIVKELFIINKSELTPFVLENDVNVNEDTRLTYRYLDLRRQVMQNNLIIRAKINHIIRNFLTDLNFLEVETPYFAKSTPEGARDFLVPSRLNKNKFYALPQSPQLFKQLLMISGIDRYYQIVRCFRDEDLRIDRQPEFTQLDLEMSFATSEDVMQISESLIKKILKEVKNFEIKEPLLRLSYKDAIDLYGSDKPDLRYDLKIHTLNDIFKNTNIKFLNNPDLFIRAICIDQLLSKKQLEDLNQQAKQFSFNSIAFIKFEDNNWSGSLASQLTENEKELLIKEFDIKNKATIILNIGKYAEISQLMGAIRISLAKMFNLETKDDFKLLWVVDFPLFEFSEQENRYVAAHHPFTSPKEECLTDFDTNKKDALACAYDLVMNGFEIGGGSQRITNPEIQQRMFDAVELTAKQVETNFGWFMNAYKYGAPYHAGIAWGLDRISMILTDSNSIRDVIAFPKNSLGIDMMSNAPDLVSEKQLEELNIKIVK"
     primer_bind     complement(1847..1863)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1930..2518)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2689..3549)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3550..3654)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /translation="MKRTHTCGELTIDNIDQEVILQGWVKKIRKLGAMVFIDLKDRYGITQLVIEQENIDLINNVKNEYVIEIKGNVVKRKSINKELVTGDIEVIVKELFIINKSELTPFVLENDVNVNEDTRLTYRYLDLRRQVMQNNLIIRAKINHIIRNFLTDLNFLEVETPYFAKSTPEGARDFLVPSRLNKNKFYALPQSPQLFKQLLMISGIDRYYQIVRCFRDEDLRIDRQPEFTQLDLEMSFATSEDVMQISESLIKKILKEVKNFEIKEPLLRLSYKDAIDLYGSDKPDLRYDLKIHTLNDIFKNTNIKFLNNPDLFIRAICIDQLLSKKQLEDLNQQAKQFSFNSIAFIKFEDNNWSGSLASQLTENEKELLIKEFDIKNKATIILNIGKYAEISQLMGAIRISLAKMFNLETKDDFKLLWVVDFPLFEFSEQENRYVAAHHPFTSPKEECLTDFDTNKKDALACAYDLVMNGFEIGGGSQRITNPEIQQRMFDAVELTAKQVETNFGWFMNAYKYGAPYHAGIAWGLDRISMILTDSNSIRDVIAFPKNSLGIDMMSNAPDLVSEKQLEELNIKIVK"
                     /label="MMSYN1_0287"
                     /note="AspRS: Aspartyl-tRNA synthetase"
ORIGIN
        1 ATGAAACGTA CGCACACATG TGGTGAGCTG ACTATCGATA ACATTGACCA AGAAGTTATT
       61 CTGCAGGGTT GGGTTAAGAA GATTCGTAAA TTGGGGGCAA TGGTATTTAT TGACTTGAAA
      121 GACCGCTACG GCATCACTCA GTTAGTGATT GAGCAGGAGA ACATTGATTT GATTAATAAC
      181 GTAAAAAATG AGTATGTAAT CGAAATTAAG GGAAACGTCG TGAAACGTAA GTCGATCAAT
      241 AAAGAATTAG TGACCGGCGA CATCGAAGTT ATCGTAAAGG AGTTATTTAT TATTAATAAA
      301 TCTGAACTGA CCCCATTCGT ACTTGAGAAC GACGTTAACG TGAATGAAGA TACGCGCTTA
      361 ACCTATCGCT ACTTAGACTT GCGTCGTCAG GTTATGCAAA ATAATCTGAT TATTCGCGCC
      421 AAAATCAACC ACATCATTCG TAACTTCCTT ACGGATCTTA ACTTCTTAGA GGTTGAAACC
      481 CCGTACTTTG CCAAGTCAAC ACCTGAAGGG GCGCGTGACT TCCTGGTTCC GAGTCGCCTG
      541 AACAAAAACA AGTTCTATGC ACTGCCCCAA TCGCCCCAAT TATTTAAACA GTTGCTTATG
      601 ATTAGCGGCA TCGACCGTTA TTACCAGATC GTGCGCTGCT TTCGTGACGA GGACTTGCGC
      661 ATCGACCGTC AACCTGAATT TACTCAACTT GACTTGGAGA TGTCTTTTGC GACGTCTGAA
      721 GATGTCATGC AGATTAGCGA GAGCTTGATT AAGAAGATTT TGAAGGAGGT CAAAAACTTC
      781 GAAATTAAAG AACCATTGTT GCGTTTGAGT TACAAAGATG CGATTGATTT GTATGGTTCA
      841 GACAAACCCG ATTTACGCTA CGACCTGAAG ATCCACACAC TGAACGACAT TTTCAAGAAT
      901 ACGAATATTA AATTTCTGAA CAATCCAGAT TTATTTATCC GCGCGATTTG TATTGACCAG
      961 CTTTTGTCAA AGAAGCAATT AGAAGATCTG AATCAACAGG CAAAACAATT CTCTTTCAAC
     1021 AGTATCGCCT TCATCAAATT CGAGGACAAT AACTGGTCTG GATCTTTGGC CAGTCAGTTG
     1081 ACTGAAAATG AAAAAGAACT TCTGATTAAG GAGTTCGATA TCAAAAACAA AGCAACCATC
     1141 ATCTTGAACA TTGGGAAGTA TGCTGAGATT AGCCAGTTGA TGGGTGCCAT TCGCATTTCG
     1201 TTAGCAAAGA TGTTTAACCT GGAAACCAAG GATGACTTCA AGTTGCTGTG GGTGGTTGAC
     1261 TTTCCGTTAT TCGAATTTTC GGAGCAGGAG AATCGCTATG TCGCCGCGCA CCACCCGTTC
     1321 ACTAGCCCCA AAGAGGAATG TCTGACTGAT TTTGATACTA ACAAAAAAGA TGCGCTTGCT
     1381 TGCGCGTACG ATCTTGTTAT GAATGGCTTT GAAATCGGTG GGGGCAGCCA GCGCATCACC
     1441 AATCCGGAAA TCCAACAACG CATGTTCGAC GCTGTCGAGT TAACTGCCAA ACAAGTTGAA
     1501 ACCAATTTCG GCTGGTTTAT GAATGCCTAC AAATATGGTG CTCCGTACCA CGCTGGTATT
     1561 GCTTGGGGCC TGGACCGTAT CAGTATGATC CTTACAGACA GCAACTCGAT TCGCGACGTT
     1621 ATCGCCTTCC CAAAGAATTC GCTTGGTATC GACATGATGT CGAATGCACC TGACTTGGTT
     1681 TCAGAGAAGC AACTGGAAGA GTTAAATATC AAGATCGTGA AGTGAAGAGC TTAGAGACCG
     1741 GTACTAGTTC CTGCAGGGCT CGAGGTGTCA ATCGTCGGAG CCGCTGAGCA ATAACTAGCA
     1801 TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCATGGTCA TAGCTGTTTC
     1861 CTGAGAGCTT GGCAGGTGAT GACACACATT AACAAATTTC GTGAGGAGTC TCCAGAAGAA
     1921 TGCCATTAAT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG
     1981 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC
     2041 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC
     2101 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT
     2161 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT
     2221 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC
     2281 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA
     2341 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA
     2401 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG
     2461 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA
     2521 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG
     2581 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG
     2641 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT
     2701 AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT
     2761 CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT
     2821 GATACCGCGA GAACCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG
     2881 AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG
     2941 TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT
     3001 TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC
     3061 CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT
     3121 CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC
     3181 AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA
     3241 GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC
     3301 GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA
     3361 ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA
     3421 ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG
     3481 AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG
     3541 AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT
     3601 GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACCTG
     3661 CACCAGTCAG TAAAACGACG GCCAGTAGTC AAAAGCCTCC GACCGGAGGC TTTTGACTTG
     3721 GTCAGGTGGA GTGAATTCGC GGCCGCTTCT AGAGGCTCTT CGTAATTACG AAGAGGTCTC
     3781 TA
//