LOCUS Exported 3302 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3302 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 1315..1362 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 3190..3206 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 3207..3238 /note="bidirectional E. coli tonB-P14 transcription terminator" /label="tonB terminator" CDS 1..0 /label="MMSYN1_0288" /note="" /translation="MLQKPRGTQDFFLDEAKLWNKVETKLKEILDQFNYSEIRTPMFESKELFIRSIGSTTDIVSKEMYEFVDKKNRSLVLKPEGTASVVRAVIENKLYKEENLPLKVYYISPMFRYERPQNGRYRQFHQLGIEVFGSDSIQQDYEVLNIATKIINQFKLNENIKIYTNFLITGKNREDYILELKKYLSDFKLCNDCNTRLEKNPLRVLDCKIDDKQFKNVPSMQDFLTKEQKTRYDQTLELFKKTNISVIHDDKLVRGLDYYTGFIFEIKYLNNNNEQTIIAGGRYNNLVNEIGNINLAACGFGMGLERFINIIKEQNSSLVNQKTNIDLYTICIDDLAIELNQQILDLTRSIGLKADSNYYHLSLKSALKKADKLNPKYVIILGSNEAKTNEFIIKDQINKTQIKTTLTKFIKYLK" primer_bind complement(1367..1383) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" rep_origin complement(1450..2038) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2209..3069) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(3070..3174) /note="ampicillin resistance gene promoter" /label="bla promoter" CDS complement(1..0) /label="MMSYN1_0288" /note="" /translation="MLQKPRGTQDFFLDEAKLWNKVETKLKEILDQFNYSEIRTPMFESKELFIRSIGSTTDIVSKEMYEFVDKKNRSLVLKPEGTASVVRAVIENKLYKEENLPLKVYYISPMFRYERPQNGRYRQFHQLGIEVFGSDSIQQDYEVLNIATKIINQFKLNENIKIYTNFLITGKNREDYILELKKYLSDFKLCNDCNTRLEKNPLRVLDCKIDDKQFKNVPSMQDFLTKEQKTRYDQTLELFKKTNISVIHDDKLVRGLDYYTGFIFEIKYLNNNNEQTIIAGGRYNNLVNEIGNINLAACGFGMGLERFINIIKEQNSSLVNQKTNIDLYTICIDDLAIELNQQILDLTRSIGLKADSNYYHLSLKSALKKADKLNPKYVIILGSNEAKTNEFIIKDQINKTQIKTTLTKFIKYLK" ORIGIN 1 ATGTTACAAA AACCTCGCGG GACACAGGAC TTCTTTTTAG ATGAAGCCAA ACTGTGGAAC 61 AAAGTCGAAA CGAAACTTAA GGAGATCCTG GATCAGTTTA ACTATAGCGA GATCCGTACA 121 CCTATGTTCG AGAGTAAGGA GCTGTTTATC CGTTCCATTG GGTCGACAAC GGATATCGTT 181 TCAAAAGAGA TGTACGAATT CGTAGATAAA AAGAATCGCA GTCTTGTGTT GAAACCCGAA 241 GGCACTGCAT CCGTCGTGCG CGCAGTGATT GAGAACAAGT TGTACAAAGA GGAGAACTTA 301 CCCTTGAAGG TCTATTACAT CAGTCCCATG TTTCGCTATG AACGCCCCCA GAATGGACGC 361 TACCGCCAAT TTCATCAGTT GGGAATTGAA GTGTTCGGCT CAGACTCAAT CCAGCAAGAC 421 TATGAGGTCC TTAATATCGC TACTAAAATC ATTAATCAGT TCAAATTGAA TGAGAACATC 481 AAAATTTATA CTAATTTTCT GATTACAGGG AAAAACCGCG AGGATTATAT TCTTGAATTG 541 AAGAAATATT TATCGGATTT CAAACTTTGC AATGACTGTA ATACGCGCCT GGAGAAAAAT 601 CCACTTCGTG TCTTGGACTG TAAAATCGAT GATAAGCAAT TCAAAAACGT ACCGTCGATG 661 CAGGACTTTC TTACCAAAGA GCAAAAGACT CGCTATGACC AGACATTGGA ACTTTTCAAA 721 AAGACTAATA TCTCCGTCAT CCATGATGAT AAACTGGTAC GCGGACTTGA CTACTATACC 781 GGCTTTATTT TCGAGATCAA ATACTTGAAT AATAACAACG AGCAGACTAT TATCGCTGGA 841 GGACGCTATA ATAACCTGGT TAACGAGATC GGCAATATTA ATTTAGCCGC GTGTGGGTTC 901 GGTATGGGGT TAGAACGCTT CATTAACATC ATCAAAGAGC AAAACTCCTC CCTTGTCAAT 961 CAAAAGACGA ATATCGATTT GTACACTATC TGCATTGACG ACTTAGCAAT TGAGCTGAAT 1021 CAGCAGATCT TGGATCTGAC TCGTTCGATT GGTTTAAAGG CCGACAGTAA TTATTACCAT 1081 TTGTCGTTGA AGAGTGCCTT AAAGAAAGCA GATAAACTTA ATCCGAAATA CGTCATTATC 1141 TTGGGGTCGA ACGAAGCGAA AACCAACGAG TTCATTATTA AGGATCAGAT CAACAAAACT 1201 CAAATCAAAA CTACACTGAC GAAATTTATT AAGTATCTTA AGTGAAGAGC TTAGAGACCG 1261 GTACTAGTTC CTGCAGGGCT CGAGGTGTCA ATCGTCGGAG CCGCTGAGCA ATAACTAGCA 1321 TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCATGGTCA TAGCTGTTTC 1381 CTGAGAGCTT GGCAGGTGAT GACACACATT AACAAATTTC GTGAGGAGTC TCCAGAAGAA 1441 TGCCATTAAT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 1501 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 1561 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 1621 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 1681 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 1741 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 1801 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 1861 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 1921 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 1981 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 2041 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 2101 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 2161 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT 2221 AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT 2281 CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT 2341 GATACCGCGA GAACCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG 2401 AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG 2461 TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT 2521 TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC 2581 CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT 2641 CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC 2701 AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA 2761 GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC 2821 GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA 2881 ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA 2941 ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG 3001 AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG 3061 AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT 3121 GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACCTG 3181 CACCAGTCAG TAAAACGACG GCCAGTAGTC AAAAGCCTCC GACCGGAGGC TTTTGACTTG 3241 GTCAGGTGGA GTGAATTCGC GGCCGCTTCT AGAGGCTCTT CGTAATTACG AAGAGGTCTC 3301 TA //