LOCUS       Exported     2936 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2936
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      949..996
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
                     /label="T7 terminator"
     primer_bind     2824..2840
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2841..2872
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0290"
                     /note="TruB: tRNA pseudouridine(55) synthase"
                     /translation="MQKSGIFILNKPSNISTYQLINQVKKKLNIKKVGHCGTLDLLATGVVICLVNNATKISDYLLNANKAYQVKIKLFTLTDSFDAEGNIIQTQTPFNISLDQINKVISKYNNYTYNQCPPIYSSIKVDGKKLYEYALTNQDVEIKSRKVTIYKTSLLDYDQKNYEIFLDVKCSKGTYIRSLAVDICKDLNTIGYVVYLNRTLSGNFKLENATDLNNISWDHLISINDAIKTNDFKVVNYQNSLEIIQGKKIILNDINDDLVFISDDQINILAVYQKYDNNIFKIKRGGLNNDIY"
     primer_bind     complement(1001..1017)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(1084..1672)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1843..2703)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2704..2808)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0290"
                     /note="TruB: tRNA pseudouridine(55) synthase"
                     /translation="MQKSGIFILNKPSNISTYQLINQVKKKLNIKKVGHCGTLDLLATGVVICLVNNATKISDYLLNANKAYQVKIKLFTLTDSFDAEGNIIQTQTPFNISLDQINKVISKYNNYTYNQCPPIYSSIKVDGKKLYEYALTNQDVEIKSRKVTIYKTSLLDYDQKNYEIFLDVKCSKGTYIRSLAVDICKDLNTIGYVVYLNRTLSGNFKLENATDLNNISWDHLISINDAIKTNDFKVVNYQNSLEIIQGKKIILNDINDDLVFISDDQINILAVYQKYDNNIFKIKRGGLNNDIY"
ORIGIN
        1 ATGCAAAAAA GTGGAATTTT CATCCTGAAC AAGCCCAGCA ATATCTCAAC ATATCAGTTG
       61 ATTAACCAAG TCAAAAAGAA GCTTAATATC AAGAAAGTAG GCCACTGCGG TACTCTTGAT
      121 TTATTAGCGA CTGGTGTTGT GATCTGCTTA GTAAATAATG CAACGAAGAT TTCTGATTAC
      181 CTTCTGAACG CCAACAAAGC GTACCAAGTA AAAATTAAAT TGTTTACCTT AACCGATAGT
      241 TTCGACGCAG AAGGCAACAT CATTCAAACC CAAACTCCGT TTAATATTTC GCTTGATCAA
      301 ATTAACAAGG TTATTAGTAA ATACAACAAC TATACCTATA ATCAGTGTCC TCCCATTTAT
      361 TCTTCTATTA AGGTAGACGG GAAAAAATTG TACGAATATG CACTGACTAA CCAGGATGTT
      421 GAGATCAAGA GCCGTAAGGT TACGATTTAC AAAACATCTC TTCTGGACTA CGACCAGAAG
      481 AATTATGAGA TCTTCCTGGA TGTCAAGTGC AGTAAGGGGA CATACATCCG TTCGTTAGCG
      541 GTTGACATTT GCAAAGATTT GAACACTATC GGCTACGTGG TCTACTTAAA TCGCACTTTG
      601 TCTGGGAATT TCAAACTGGA GAATGCGACC GACTTAAACA ACATTTCGTG GGACCATCTG
      661 ATTTCTATCA ACGATGCTAT CAAAACCAAC GATTTCAAGG TCGTTAATTA CCAGAACTCG
      721 CTTGAGATTA TCCAAGGAAA AAAGATTATT CTGAACGACA TTAACGACGA CTTGGTTTTT
      781 ATTTCAGACG ACCAAATTAA TATCTTGGCA GTTTATCAGA AATATGACAA TAACATTTTC
      841 AAGATTAAGC GTGGCGGGCT GAACAATGAC ATCTACTGAA GAGCTTAGAG ACCGGTACTA
      901 GTTCCTGCAG GGCTCGAGGT GTCAATCGTC GGAGCCGCTG AGCAATAACT AGCATAACCC
      961 CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCATG GTCATAGCTG TTTCCTGAGA
     1021 GCTTGGCAGG TGATGACACA CATTAACAAA TTTCGTGAGG AGTCTCCAGA AGAATGCCAT
     1081 TAATTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG
     1141 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT
     1201 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG
     1261 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG
     1321 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG
     1381 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC
     1441 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG
     1501 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT
     1561 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG
     1621 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC
     1681 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT
     1741 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT
     1801 TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG
     1861 TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT
     1921 CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC
     1981 GCGAGAACCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC
     2041 CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG
     2101 GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC
     2161 AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG
     2221 ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC
     2281 TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT
     2341 GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC
     2401 AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT
     2461 ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC
     2521 TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC
     2581 TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA
     2641 AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT
     2701 CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG
     2761 ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CCTGCACCAG
     2821 TCAGTAAAAC GACGGCCAGT AGTCAAAAGC CTCCGACCGG AGGCTTTTGA CTTGGTCAGG
     2881 TGGAGTGAAT TCGCGGCCGC TTCTAGAGGC TCTTCGTAAT TACGAAGAGG TCTCTA
//