LOCUS       Exported     3778 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3778
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
                     /label="T7 terminator"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2010..3764
                     /label="MMSYN1_0300"
                     /note="NusA: Transcription terminationantitermination protein"
                     /translation="MLNGTELLESIKLIEKEKGISKESIINGLKEGLQKAYERFYDTDAIIKIDINENTGLITMHQELKVVDDEQLDDDWLEITLSKAKLKNPDIQIGDTIYKPIEFSEEFSRMVVNQVRQIFQQKIREAERARIYEQFVSLEGEVVQAKVVGMNRENNYVLDINGTTAYLWKSKTINNEIFQINEIIDVYIEVVEKESKLSQISISRTAPNFLTKLIEREVPEVRMGIVEIKAVSREPGKRSKVAVITHNNNVEPIGAIIGVGGNRINRISDILKGEKIDIIRWDEDQITYLINAMTPVKVISINKIGDEYDIVVPDTQLSLAIGKQGVAAKLIASLLKTKINIFSYSTALKENMDILWNGDTTIQEVETNTYTPKTKATKKEEKPVITTTKKPIKQTTKKEENQIDVDALIAFQAEVEHEQELKDQEELLKQESMYKEYENNFNDFENEKEILLAEKQLETQNQIIKEPVVEVQEFEIEKQSKIEDQITENKQPEIKTEVETKPNIVEQVNKLNTNKPNNKFEQNRFNYKKQKQKEEELELDFDIKNEPDIDEIDANLKAFNDAILKQEDDEDLDIDLDDYDKYYD"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGCTTAACGG CACTGAGCTT TTAGAAAGCA
     2041 TTAAACTTAT CGAAAAGGAG AAAGGGATTT CTAAAGAGAG CATCATTAAC GGCTTAAAGG
     2101 AGGGATTACA GAAGGCTTAT GAGCGCTTTT ATGATACCGA CGCAATCATT AAAATTGATA
     2161 TTAATGAGAA TACCGGGCTG ATCACCATGC ACCAAGAATT AAAAGTAGTA GATGACGAAC
     2221 AGCTGGATGA TGACTGGCTG GAAATTACCC TGTCCAAGGC GAAGCTGAAG AATCCTGACA
     2281 TCCAGATCGG CGACACTATC TACAAACCCA TCGAGTTTAG CGAGGAATTT TCGCGCATGG
     2341 TAGTAAATCA GGTGCGTCAA ATCTTCCAGC AGAAAATCCG TGAAGCTGAG CGCGCACGCA
     2401 TCTATGAGCA ATTTGTGAGT TTGGAAGGTG AGGTCGTTCA GGCGAAAGTA GTTGGGATGA
     2461 ATCGTGAAAA CAATTATGTG TTGGACATCA ATGGTACTAC CGCCTACCTG TGGAAGTCCA
     2521 AAACCATCAA CAATGAAATC TTCCAAATTA ACGAAATTAT CGATGTATAT ATCGAGGTGG
     2581 TGGAGAAAGA ATCTAAACTG TCACAAATCT CCATCTCTCG CACTGCACCT AATTTTTTAA
     2641 CGAAACTTAT TGAGCGTGAA GTACCCGAGG TTCGCATGGG AATCGTTGAA ATTAAGGCCG
     2701 TTTCACGTGA ACCTGGCAAA CGCTCCAAGG TCGCCGTCAT TACTCACAAC AATAATGTGG
     2761 AACCGATCGG CGCGATCATC GGCGTTGGTG GAAATCGCAT CAACCGTATT AGTGACATCT
     2821 TAAAGGGGGA AAAGATCGAC ATCATTCGTT GGGATGAGGA CCAGATTACA TACCTTATCA
     2881 ACGCAATGAC TCCGGTCAAA GTCATTAGTA TTAATAAAAT TGGAGATGAA TACGATATTG
     2941 TAGTACCCGA TACGCAGCTG AGTTTGGCGA TTGGAAAGCA GGGCGTGGCG GCCAAGTTAA
     3001 TTGCGAGTTT ATTAAAGACC AAGATCAATA TCTTTTCATA CTCTACGGCG TTAAAAGAGA
     3061 ATATGGATAT TTTGTGGAAT GGGGATACGA CGATCCAAGA AGTAGAAACG AATACATACA
     3121 CGCCAAAGAC TAAAGCAACC AAAAAGGAGG AGAAGCCGGT TATCACTACA ACTAAGAAGC
     3181 CAATTAAGCA AACTACAAAG AAAGAGGAGA ACCAAATCGA TGTTGACGCC TTGATCGCTT
     3241 TCCAAGCCGA AGTTGAGCAC GAACAGGAAC TGAAGGACCA AGAGGAGCTG CTTAAACAGG
     3301 AGTCTATGTA CAAAGAATAC GAGAATAATT TTAACGACTT TGAAAATGAA AAAGAAATCC
     3361 TGTTGGCGGA AAAGCAGCTG GAAACGCAGA ATCAGATTAT TAAGGAGCCT GTCGTGGAAG
     3421 TACAGGAATT TGAGATCGAG AAACAAAGCA AGATTGAGGA TCAGATTACT GAGAATAAGC
     3481 AGCCGGAAAT TAAGACAGAG GTCGAAACCA AACCTAATAT TGTGGAACAG GTCAATAAAT
     3541 TGAATACAAA CAAGCCCAAT AACAAATTCG AACAAAATCG CTTCAATTAC AAGAAACAAA
     3601 AGCAGAAAGA GGAGGAACTG GAGCTGGATT TTGACATTAA GAACGAGCCG GACATCGATG
     3661 AAATCGACGC AAATCTTAAG GCTTTTAACG ATGCGATTTT GAAACAAGAA GATGACGAGG
     3721 ACCTTGATAT CGACTTGGAT GATTACGATA AGTATTATGA TTGAAGAGCT TAGAGACC
//