LOCUS       Exported     3134 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3134
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      1147..1194
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3022..3038
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3039..3070
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /translation="MTKHEIINELLEKNNADAILLYSPENRYWFSKFHSSLGYLIITKTQSHLFLDGRYITAARNNKNINKDIELHHFSKNLKQDLIDILNQNNVKTLAFESDWTYFEQYQAYKNHWFKDFDLIGINCSKIRMIKDDWEIANIKKACDITDQVFQAALDFIKPGITEKQLQRFIDDKFLEFGADKISFDTIIASGVNGSMPHAVPSDKVINNNELITIDMGCFYNGYCSDQTRTIALGDVDPKLVEIYNIVYEAQSLGISLVKEGVIAGDIHKQVYDFIDKKGYGKYFDHGLGHGIGVEIHEEPSVGSTGSEVLKENMTITIEPGIYIPDLGGVRIEDDVLVTKTGCKLLTSSPRILLKLQK"
                     /label="MMSYN1_0305"
                     /note=""
     primer_bind     complement(1199..1215)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1282..1870)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2041..2901)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2902..3006)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0305"
                     /note=""
                     /translation="MTKHEIINELLEKNNADAILLYSPENRYWFSKFHSSLGYLIITKTQSHLFLDGRYITAARNNKNINKDIELHHFSKNLKQDLIDILNQNNVKTLAFESDWTYFEQYQAYKNHWFKDFDLIGINCSKIRMIKDDWEIANIKKACDITDQVFQAALDFIKPGITEKQLQRFIDDKFLEFGADKISFDTIIASGVNGSMPHAVPSDKVINNNELITIDMGCFYNGYCSDQTRTIALGDVDPKLVEIYNIVYEAQSLGISLVKEGVIAGDIHKQVYDFIDKKGYGKYFDHGLGHGIGVEIHEEPSVGSTGSEVLKENMTITIEPGIYIPDLGGVRIEDDVLVTKTGCKLLTSSPRILLKLQK"
ORIGIN
        1 ATGACGAAGC ACGAAATCAT TAACGAATTA CTGGAGAAAA ACAACGCTGA CGCAATCTTG
       61 TTATATTCGC CGGAGAATCG CTATTGGTTT TCCAAGTTCC ATTCAAGCCT TGGCTATCTT
      121 ATCATTACAA AAACGCAGTC ACACCTGTTC TTAGACGGTC GCTATATCAC CGCCGCCCGT
      181 AACAACAAAA ATATTAACAA AGACATCGAA CTTCACCATT TCAGCAAAAA TCTGAAGCAA
      241 GATTTGATCG ATATTCTGAA CCAGAACAAT GTGAAAACAT TGGCTTTTGA GAGCGACTGG
      301 ACATACTTTG AACAATACCA AGCATATAAG AACCACTGGT TCAAAGACTT CGATTTGATT
      361 GGGATCAATT GCAGCAAGAT CCGTATGATC AAGGATGACT GGGAGATCGC AAATATCAAA
      421 AAGGCTTGCG ACATTACCGA CCAGGTGTTT CAAGCCGCAT TAGACTTTAT CAAGCCGGGC
      481 ATCACAGAGA AACAACTTCA GCGTTTCATC GATGACAAGT TTCTTGAATT CGGCGCTGAC
      541 AAGATCAGTT TCGATACGAT CATTGCTTCA GGGGTCAATG GAAGTATGCC TCACGCTGTT
      601 CCCTCCGATA AAGTGATCAA TAATAACGAG TTGATCACCA TTGATATGGG CTGTTTTTAT
      661 AACGGGTACT GCTCCGATCA GACCCGCACT ATCGCTTTAG GCGACGTAGA TCCTAAGCTG
      721 GTCGAAATCT ATAATATCGT CTATGAAGCC CAAAGTTTAG GTATTAGCCT GGTAAAAGAG
      781 GGGGTCATTG CCGGCGACAT CCACAAACAG GTGTACGATT TTATCGATAA AAAGGGATAT
      841 GGGAAGTACT TTGACCATGG TCTTGGTCAC GGGATTGGGG TGGAAATCCA CGAGGAGCCC
      901 TCGGTAGGCT CTACGGGAAG TGAAGTCCTG AAAGAAAACA TGACTATTAC AATTGAGCCG
      961 GGTATCTATA TTCCAGACCT TGGGGGAGTA CGCATTGAGG ACGACGTATT GGTGACAAAG
     1021 ACGGGCTGCA AACTTTTGAC GAGTTCACCG CGCATCCTTT TGAAATTGCA AAAGTGAAGA
     1081 GCTTAGAGAC CGGTACTAGT TCCTGCAGGG CTCGAGGTGT CAATCGTCGG AGCCGCTGAG
     1141 CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCATGGT
     1201 CATAGCTGTT TCCTGAGAGC TTGGCAGGTG ATGACACACA TTAACAAATT TCGTGAGGAG
     1261 TCTCCAGAAG AATGCCATTA ATTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA
     1321 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC
     1381 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC
     1441 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG
     1501 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA
     1561 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC
     1621 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC
     1681 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG
     1741 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA
     1801 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA
     1861 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA
     1921 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT
     1981 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG
     2041 TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT
     2101 AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC
     2161 CAGTGCTGCA ATGATACCGC GAGAACCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA
     2221 CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA
     2281 GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA
     2341 CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT
     2401 CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC
     2461 GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT
     2521 CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC
     2581 TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG
     2641 CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT
     2701 CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC
     2761 CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG
     2821 CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC
     2881 ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG
     2941 TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT
     3001 TCCGCGCACC TGCACCAGTC AGTAAAACGA CGGCCAGTAG TCAAAAGCCT CCGACCGGAG
     3061 GCTTTTGACT TGGTCAGGTG GAGTGAATTC GCGGCCGCTT CTAGAGGCTC TTCGTAATTA
     3121 CGAAGAGGTC TCTA
//