LOCUS       Exported     3068 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3068
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1081..1128
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2956..2972
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2973..3004
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0308"
                     /note="TrpRS: Tryptophanyl-tRNA synthetase"
                     /translation="MQKQIMVSGITPSGTMTLGNYLGVVKRFIQYQNEYDLFIFIANLHAITLPQEKEKLKKNTKEIAALYFACGLDINKTTIFLQSDVLEHAQLGWILTTNTSMGELSRMTQFKDKSLKAESINGKGYIPTGLFTYPALMAADILLYDPKYVPVGIDQKQHIEITRDIAIRMNNKYGEMFKIPEPLINSEIKIMDLQDPTKKMSKSSTNPKAIITMLDSIDEIKSKIKAAVTDSENLIKYDPINKPGVSNLITIYCQLKNISIKQAEKHWENKNYKDLKDDVTQVLIDEIIPIQTKFKELYNSKQVEQWLELGANKARYIANKKLNKVQNLMGLNYNRK"
     primer_bind     complement(1133..1149)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1216..1804)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1975..2835)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(2836..2940)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
     CDS             complement(1..0)
                     /note="TrpRS: Tryptophanyl-tRNA synthetase"
                     /translation="MQKQIMVSGITPSGTMTLGNYLGVVKRFIQYQNEYDLFIFIANLHAITLPQEKEKLKKNTKEIAALYFACGLDINKTTIFLQSDVLEHAQLGWILTTNTSMGELSRMTQFKDKSLKAESINGKGYIPTGLFTYPALMAADILLYDPKYVPVGIDQKQHIEITRDIAIRMNNKYGEMFKIPEPLINSEIKIMDLQDPTKKMSKSSTNPKAIITMLDSIDEIKSKIKAAVTDSENLIKYDPINKPGVSNLITIYCQLKNISIKQAEKHWENKNYKDLKDDVTQVLIDEIIPIQTKFKELYNSKQVEQWLELGANKARYIANKKLNKVQNLMGLNYNRK"
                     /label="MMSYN1_0308"
ORIGIN
        1 ATGCAGAAGC AGATCATGGT TTCTGGTATT ACCCCATCGG GCACTATGAC CCTGGGCAAT
       61 TACTTGGGTG TCGTAAAGCG TTTCATCCAA TATCAGAATG AATATGACCT TTTTATCTTT
      121 ATCGCTAATC TTCATGCGAT TACTTTGCCT CAGGAAAAGG AGAAACTGAA GAAAAACACG
      181 AAGGAGATTG CTGCGCTTTA CTTCGCATGT GGGTTGGATA TCAATAAGAC AACTATTTTC
      241 TTGCAGTCGG ATGTTCTGGA GCACGCCCAG TTGGGGTGGA TTTTAACAAC TAACACATCG
      301 ATGGGTGAGT TGTCGCGTAT GACACAATTT AAAGATAAGT CGTTGAAGGC AGAGAGTATC
      361 AACGGTAAAG GTTATATTCC TACCGGACTG TTTACATACC CGGCATTAAT GGCAGCTGAC
      421 ATCTTGTTGT ATGACCCTAA ATATGTCCCC GTGGGGATCG ATCAAAAGCA ACACATTGAA
      481 ATTACGCGCG ATATCGCCAT CCGTATGAAC AACAAGTACG GAGAAATGTT CAAAATTCCC
      541 GAGCCTTTGA TCAATAGTGA AATCAAAATC ATGGACTTAC AAGACCCGAC TAAGAAGATG
      601 AGTAAGTCGT CAACTAACCC CAAGGCTATT ATTACAATGT TAGATTCTAT CGATGAGATT
      661 AAAAGCAAAA TTAAGGCCGC AGTTACCGAT AGCGAGAATC TTATTAAATA TGATCCGATC
      721 AATAAGCCCG GTGTCTCTAA TCTGATTACA ATTTATTGTC AGTTAAAGAA CATTAGTATT
      781 AAGCAAGCAG AGAAACATTG GGAAAATAAA AATTACAAAG ATTTAAAAGA TGATGTCACC
      841 CAAGTTCTTA TTGACGAGAT CATTCCTATC CAAACAAAGT TCAAGGAACT TTATAATAGC
      901 AAACAGGTGG AACAGTGGCT GGAGCTTGGC GCCAACAAGG CCCGTTATAT CGCAAACAAA
      961 AAACTGAATA AAGTGCAAAA CTTGATGGGC TTAAACTATA ACCGTAAGTG AAGAGCTTAG
     1021 AGACCGGTAC TAGTTCCTGC AGGGCTCGAG GTGTCAATCG TCGGAGCCGC TGAGCAATAA
     1081 CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCA TGGTCATAGC
     1141 TGTTTCCTGA GAGCTTGGCA GGTGATGACA CACATTAACA AATTTCGTGA GGAGTCTCCA
     1201 GAAGAATGCC ATTAATTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG
     1261 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG
     1321 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT
     1381 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT
     1441 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG
     1501 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT
     1561 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT
     1621 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT
     1681 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC
     1741 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC
     1801 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG
     1861 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA
     1921 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA
     1981 ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC
     2041 CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC
     2101 TGCAATGATA CCGCGAGAAC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC
     2161 AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT
     2221 TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT
     2281 TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC
     2341 CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG
     2401 CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT
     2461 TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC
     2521 TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG
     2581 CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT
     2641 TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC
     2701 GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC
     2761 TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA
     2821 ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG
     2881 TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG
     2941 CACCTGCACC AGTCAGTAAA ACGACGGCCA GTAGTCAAAA GCCTCCGACC GGAGGCTTTT
     3001 GACTTGGTCA GGTGGAGTGA ATTCGCGGCC GCTTCTAGAG GCTCTTCGTA ATTACGAAGA
     3061 GGTCTCTA
//