LOCUS       Exported     2792 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2792
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      805..852
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2680..2696
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2697..2728
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0314"
                     /note=""
                     /translation="MLFFLTNGAAICVIILLFAIAYMMDPKFLKTITTTKITMMAMQVALIVLLTNFLGYSGVFGARLMLGNFILFLSGMLFGPMGGALVGALSYTAGMVNPGIFIHFSFMAAYMIYAMLGSLVFIKKQKSRLSFMISVFVLLFIASFTLTFISHPIAMLAIGKNAYVYVTLVKKFIVFPIDAVIEPILIISTFEVSILVLKRVPNTWNQLWCTRFDSLEFLNKQEKKSKKDLKITQNEPIITSQASN"
     primer_bind     complement(857..873)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(940..1528)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1699..2559)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(2560..2664)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0314"
                     /note=""
                     /translation="MLFFLTNGAAICVIILLFAIAYMMDPKFLKTITTTKITMMAMQVALIVLLTNFLGYSGVFGARLMLGNFILFLSGMLFGPMGGALVGALSYTAGMVNPGIFIHFSFMAAYMIYAMLGSLVFIKKQKSRLSFMISVFVLLFIASFTLTFISHPIAMLAIGKNAYVYVTLVKKFIVFPIDAVIEPILIISTFEVSILVLKRVPNTWNQLWCTRFDSLEFLNKQEKKSKKDLKITQNEPIITSQASN"
ORIGIN
        1 ATGTTATTCT TTTTAACTAA CGGAGCTGCG ATCTGTGTTA TCATCCTTCT TTTCGCGATT
       61 GCATATATGA TGGACCCTAA ATTTCTCAAG ACAATCACCA CTACAAAAAT CACCATGATG
      121 GCCATGCAAG TCGCCTTAAT TGTTTTATTA ACGAATTTCT TAGGCTACTC TGGGGTTTTT
      181 GGTGCCCGCC TTATGCTTGG GAATTTCATT CTGTTCCTTA GTGGGATGTT GTTCGGGCCT
      241 ATGGGTGGGG CGTTGGTAGG GGCATTGAGC TATACAGCTG GAATGGTAAA TCCCGGTATT
      301 TTCATCCACT TCTCGTTCAT GGCCGCATAT ATGATTTACG CTATGTTGGG ATCCCTGGTG
      361 TTTATTAAAA AGCAAAAATC CCGCTTAAGC TTCATGATTA GTGTATTTGT TCTTCTTTTT
      421 ATTGCAAGCT TTACATTAAC ATTTATCAGT CATCCAATCG CCATGTTAGC AATTGGCAAG
      481 AATGCATACG TTTATGTCAC CTTAGTAAAG AAATTCATCG TGTTTCCTAT TGACGCTGTC
      541 ATTGAGCCAA TCCTTATTAT CTCTACCTTT GAGGTTTCTA TTTTAGTTCT TAAGCGTGTG
      601 CCTAATACCT GGAACCAGCT TTGGTGCACC CGCTTCGATA GTTTAGAGTT CCTTAATAAA
      661 CAAGAGAAAA AGAGCAAGAA GGACTTGAAG ATTACGCAGA ATGAACCAAT CATCACGTCA
      721 CAGGCGAGTA ACTGAAGAGC TTAGAGACCG GTACTAGTTC CTGCAGGGCT CGAGGTGTCA
      781 ATCGTCGGAG CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG
      841 AGGGGTTTTT TGCATGGTCA TAGCTGTTTC CTGAGAGCTT GGCAGGTGAT GACACACATT
      901 AACAAATTTC GTGAGGAGTC TCCAGAAGAA TGCCATTAAT TTCCATAGGC TCCGCCCCCC
      961 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA
     1021 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC
     1081 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC
     1141 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA
     1201 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC
     1261 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG
     1321 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG
     1381 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG
     1441 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA
     1501 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA
     1561 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT
     1621 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA
     1681 GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG
     1741 TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA
     1801 GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GAACCACGCT CACCGGCTCC
     1861 AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC
     1921 TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC
     1981 AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC
     2041 GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC
     2101 CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT
     2161 GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC
     2221 ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG
     2281 TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG
     2341 CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT
     2401 CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC
     2461 ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA
     2521 AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA
     2581 TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA
     2641 AAATAAACAA ATAGGGGTTC CGCGCACCTG CACCAGTCAG TAAAACGACG GCCAGTAGTC
     2701 AAAAGCCTCC GACCGGAGGC TTTTGACTTG GTCAGGTGGA GTGAATTCGC GGCCGCTTCT
     2761 AGAGGCTCTT CGTAATTACG AAGAGGTCTC TA
//