LOCUS Exported 3994 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3994 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 38..85 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 1933..1949 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 1950..1981 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 2010..3980 /label="MMSYN1_0316" /note="tkt: transketolase" /translation="MKTSKNDNLNALRILGVSAINKAKSGHPGIVLGAAGIVYVLFNKIMNFNPKNPEWFNRDRFILSAGHGSALLYSALHLSGYDLSIDDLKQFRQWDSKTPGHPEKTLTCGVEVTTGPLGQGVGMGVGMAVAEAHLASVYNKHDFNLIHHYTYVLCGDGDLQEGISQEAISFAGKHKLNKLILIHDSNDVQLDSNVVDVQIEDMHKRFKACNWNTIKVSDGEDLNSIYKAIRKAQLSDKPTYIEVKTIIGLGSTKQGTKDVHGAPLNDDITKVKKYFDWDYDDFIIPDSVYKHWSINAKKGEIEEEYWNQLKAKYSLKYPELSSYLDNAINKNITFDFSSLLKDIPNNDEATRLSSGKIFEKIANHEKMLIGGSADLSSSTRIKGADNQFTSLNKTGRNIMYGVREFGMGAINNGIAAHKGLIPVASGFFVFADYLKPAMRLASIMQLQQLYVFTHDSIAVGEDGPTHQPIEQLSMLRTIPNHVVLRPADYSETIACYKVALTKLTKTPTSIILTRQNVRQLQHNDVLNQVERGAYIISDQTDATVSLIASGSEVGLAIDVQKELLNHNIKAKVISMVSTSLFDKQDKEYQNLIINKNTKRIAIEMGSSAIWYKYVGDDGLVFGIDRFGESAPANSVIKEFGFTKENLTNKILEYLNK" primer_bind complement(90..106) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(173..761) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(952..1812) /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" promoter complement(1813..1917) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC 61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT 121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA 181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG 721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA 781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG 961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG 1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC 1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC 1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA 1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC 1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG 1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC 1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT 1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG 1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC 1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG 1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT 1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG 1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG 1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC 1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGAAAACCTC AAAGAACGAT AACCTTAACG 2041 CATTGCGTAT CCTGGGTGTC TCCGCCATCA ACAAGGCTAA GTCGGGACAC CCCGGGATTG 2101 TACTGGGGGC TGCCGGGATC GTCTACGTTC TGTTTAATAA AATTATGAAC TTCAATCCTA 2161 AGAATCCAGA ATGGTTCAAC CGCGATCGCT TTATTTTAAG TGCAGGACAC GGAAGTGCGT 2221 TACTGTATTC CGCACTGCAT TTATCAGGTT ATGATCTGAG TATCGACGAC TTAAAACAAT 2281 TCCGCCAGTG GGATAGTAAG ACACCCGGAC ATCCTGAAAA GACCTTGACA TGTGGGGTTG 2341 AAGTCACCAC AGGGCCATTA GGGCAGGGAG TGGGTATGGG CGTGGGGATG GCAGTAGCTG 2401 AAGCCCACCT TGCGAGCGTT TATAATAAGC ATGATTTCAA TCTGATTCAT CACTATACGT 2461 ATGTACTTTG CGGCGACGGG GATTTGCAGG AAGGCATCTC CCAAGAGGCT ATTTCTTTCG 2521 CCGGCAAACA TAAACTTAAT AAGCTTATCT TAATCCATGA TTCTAATGAC GTTCAGTTAG 2581 ATTCAAATGT TGTCGATGTT CAAATCGAAG ATATGCACAA ACGCTTCAAG GCATGTAATT 2641 GGAACACCAT CAAGGTGTCT GATGGCGAAG ATTTGAATTC AATCTACAAA GCTATCCGCA 2701 AGGCTCAATT GTCAGATAAA CCCACCTACA TTGAAGTTAA GACGATCATT GGCTTGGGTT 2761 CGACAAAGCA AGGGACAAAG GATGTTCATG GAGCACCCTT AAACGATGAC ATTACCAAGG 2821 TGAAGAAATA CTTTGATTGG GATTATGATG ACTTTATTAT TCCGGACTCA GTATACAAGC 2881 ATTGGTCAAT CAACGCTAAA AAAGGAGAAA TTGAGGAGGA ATATTGGAAT CAGTTGAAAG 2941 CTAAGTATTC GCTTAAATAT CCAGAATTAT CTTCTTATTT GGATAACGCC ATCAACAAGA 3001 ACATTACGTT CGATTTCTCC TCGCTTTTGA AAGATATTCC AAATAATGAT GAAGCTACAC 3061 GCTTATCTTC AGGTAAAATC TTTGAGAAGA TTGCTAATCA TGAAAAGATG TTAATTGGTG 3121 GAAGCGCTGA CCTGTCGAGT TCCACACGCA TTAAAGGGGC CGATAATCAG TTCACTTCCC 3181 TGAACAAAAC CGGCCGCAAT ATTATGTATG GAGTGCGCGA GTTTGGGATG GGGGCGATTA 3241 ATAATGGTAT CGCTGCGCAT AAAGGATTAA TTCCTGTAGC CTCGGGATTC TTCGTCTTTG 3301 CAGATTACTT GAAGCCGGCA ATGCGCTTAG CGTCAATCAT GCAGTTACAA CAATTATATG 3361 TGTTCACTCA CGACTCGATT GCAGTTGGAG AGGACGGTCC GACTCATCAG CCCATCGAGC 3421 AGTTGTCCAT GTTACGTACT ATTCCGAATC ACGTAGTTTT GCGTCCCGCT GACTATTCAG 3481 AAACGATTGC ATGCTATAAA GTCGCTCTTA CGAAGCTTAC TAAGACTCCA ACCTCTATCA 3541 TCCTTACTCG TCAAAACGTC CGTCAGTTGC AGCACAATGA CGTATTGAAT CAGGTGGAAC 3601 GTGGCGCCTA TATTATCTCC GACCAAACGG ATGCAACAGT TTCTCTTATC GCATCTGGGA 3661 GTGAAGTTGG TTTAGCTATT GATGTCCAGA AGGAACTGTT GAATCATAAT ATTAAAGCTA 3721 AAGTCATCTC CATGGTAAGT ACATCTCTGT TCGATAAGCA GGACAAAGAG TACCAAAATT 3781 TGATCATTAA TAAAAACACT AAACGCATTG CCATCGAAAT GGGTTCCAGT GCCATTTGGT 3841 ATAAGTACGT CGGCGACGAT GGGCTTGTTT TCGGAATCGA TCGCTTCGGT GAAAGTGCTC 3901 CTGCCAACTC AGTCATTAAG GAGTTCGGAT TCACGAAGGA AAATCTTACA AATAAAATTT 3961 TGGAGTATCT TAATAAGTGA AGAGCTTAGA GACC //