LOCUS       Exported     3994 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3994
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2010..3980
                     /label="MMSYN1_0316"
                     /note="tkt: transketolase"
                     /translation="MKTSKNDNLNALRILGVSAINKAKSGHPGIVLGAAGIVYVLFNKIMNFNPKNPEWFNRDRFILSAGHGSALLYSALHLSGYDLSIDDLKQFRQWDSKTPGHPEKTLTCGVEVTTGPLGQGVGMGVGMAVAEAHLASVYNKHDFNLIHHYTYVLCGDGDLQEGISQEAISFAGKHKLNKLILIHDSNDVQLDSNVVDVQIEDMHKRFKACNWNTIKVSDGEDLNSIYKAIRKAQLSDKPTYIEVKTIIGLGSTKQGTKDVHGAPLNDDITKVKKYFDWDYDDFIIPDSVYKHWSINAKKGEIEEEYWNQLKAKYSLKYPELSSYLDNAINKNITFDFSSLLKDIPNNDEATRLSSGKIFEKIANHEKMLIGGSADLSSSTRIKGADNQFTSLNKTGRNIMYGVREFGMGAINNGIAAHKGLIPVASGFFVFADYLKPAMRLASIMQLQQLYVFTHDSIAVGEDGPTHQPIEQLSMLRTIPNHVVLRPADYSETIACYKVALTKLTKTPTSIILTRQNVRQLQHNDVLNQVERGAYIISDQTDATVSLIASGSEVGLAIDVQKELLNHNIKAKVISMVSTSLFDKQDKEYQNLIINKNTKRIAIEMGSSAIWYKYVGDDGLVFGIDRFGESAPANSVIKEFGFTKENLTNKILEYLNK"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGAAAACCTC AAAGAACGAT AACCTTAACG
     2041 CATTGCGTAT CCTGGGTGTC TCCGCCATCA ACAAGGCTAA GTCGGGACAC CCCGGGATTG
     2101 TACTGGGGGC TGCCGGGATC GTCTACGTTC TGTTTAATAA AATTATGAAC TTCAATCCTA
     2161 AGAATCCAGA ATGGTTCAAC CGCGATCGCT TTATTTTAAG TGCAGGACAC GGAAGTGCGT
     2221 TACTGTATTC CGCACTGCAT TTATCAGGTT ATGATCTGAG TATCGACGAC TTAAAACAAT
     2281 TCCGCCAGTG GGATAGTAAG ACACCCGGAC ATCCTGAAAA GACCTTGACA TGTGGGGTTG
     2341 AAGTCACCAC AGGGCCATTA GGGCAGGGAG TGGGTATGGG CGTGGGGATG GCAGTAGCTG
     2401 AAGCCCACCT TGCGAGCGTT TATAATAAGC ATGATTTCAA TCTGATTCAT CACTATACGT
     2461 ATGTACTTTG CGGCGACGGG GATTTGCAGG AAGGCATCTC CCAAGAGGCT ATTTCTTTCG
     2521 CCGGCAAACA TAAACTTAAT AAGCTTATCT TAATCCATGA TTCTAATGAC GTTCAGTTAG
     2581 ATTCAAATGT TGTCGATGTT CAAATCGAAG ATATGCACAA ACGCTTCAAG GCATGTAATT
     2641 GGAACACCAT CAAGGTGTCT GATGGCGAAG ATTTGAATTC AATCTACAAA GCTATCCGCA
     2701 AGGCTCAATT GTCAGATAAA CCCACCTACA TTGAAGTTAA GACGATCATT GGCTTGGGTT
     2761 CGACAAAGCA AGGGACAAAG GATGTTCATG GAGCACCCTT AAACGATGAC ATTACCAAGG
     2821 TGAAGAAATA CTTTGATTGG GATTATGATG ACTTTATTAT TCCGGACTCA GTATACAAGC
     2881 ATTGGTCAAT CAACGCTAAA AAAGGAGAAA TTGAGGAGGA ATATTGGAAT CAGTTGAAAG
     2941 CTAAGTATTC GCTTAAATAT CCAGAATTAT CTTCTTATTT GGATAACGCC ATCAACAAGA
     3001 ACATTACGTT CGATTTCTCC TCGCTTTTGA AAGATATTCC AAATAATGAT GAAGCTACAC
     3061 GCTTATCTTC AGGTAAAATC TTTGAGAAGA TTGCTAATCA TGAAAAGATG TTAATTGGTG
     3121 GAAGCGCTGA CCTGTCGAGT TCCACACGCA TTAAAGGGGC CGATAATCAG TTCACTTCCC
     3181 TGAACAAAAC CGGCCGCAAT ATTATGTATG GAGTGCGCGA GTTTGGGATG GGGGCGATTA
     3241 ATAATGGTAT CGCTGCGCAT AAAGGATTAA TTCCTGTAGC CTCGGGATTC TTCGTCTTTG
     3301 CAGATTACTT GAAGCCGGCA ATGCGCTTAG CGTCAATCAT GCAGTTACAA CAATTATATG
     3361 TGTTCACTCA CGACTCGATT GCAGTTGGAG AGGACGGTCC GACTCATCAG CCCATCGAGC
     3421 AGTTGTCCAT GTTACGTACT ATTCCGAATC ACGTAGTTTT GCGTCCCGCT GACTATTCAG
     3481 AAACGATTGC ATGCTATAAA GTCGCTCTTA CGAAGCTTAC TAAGACTCCA ACCTCTATCA
     3541 TCCTTACTCG TCAAAACGTC CGTCAGTTGC AGCACAATGA CGTATTGAAT CAGGTGGAAC
     3601 GTGGCGCCTA TATTATCTCC GACCAAACGG ATGCAACAGT TTCTCTTATC GCATCTGGGA
     3661 GTGAAGTTGG TTTAGCTATT GATGTCCAGA AGGAACTGTT GAATCATAAT ATTAAAGCTA
     3721 AAGTCATCTC CATGGTAAGT ACATCTCTGT TCGATAAGCA GGACAAAGAG TACCAAAATT
     3781 TGATCATTAA TAAAAACACT AAACGCATTG CCATCGAAAT GGGTTCCAGT GCCATTTGGT
     3841 ATAAGTACGT CGGCGACGAT GGGCTTGTTT TCGGAATCGA TCGCTTCGGT GAAAGTGCTC
     3901 CTGCCAACTC AGTCATTAAG GAGTTCGGAT TCACGAAGGA AAATCTTACA AATAAAATTT
     3961 TGGAGTATCT TAATAAGTGA AGAGCTTAGA GACC
//