LOCUS       Exported     3548 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3548
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      1561..1608
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3436..3452
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3453..3484
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0325"
                     /note="membrane protein, putative"
                     /translation="MFSWDLYIINPLLIVIWLIVASYLFYKNSISKQKGLFYLEISSFWIVINFLIQIITNYIDSPILKSFSSSTLTILLFLSSYFLYATILNPFALWLTLKLQSRRIWIWISLFSCFLSVMIAFLSNVNITSIIFISLFLAVGISAQIIYFLFFNEQFNERLFPVFSSIKAGFVISFATFISYEVYSLLNLNLISNHNNYTNWIIFSLSLVCLIICLVVSIFVKERKIKVIKYKEDIVEQLQRYGYKVLIGLIVMSFLITSVNVIIKSDIFELFLVSKLKQQSYTSLNVWNYLQSFRLSFVLGQLLLGYLFYKLVIKVIGIVKSISILTSLTMFGVILITFIHNIYLLTIMMWVFGLFFFVMFYLWFGIALMWDYRSTKVSVLSTFLTVTFLTLSIWYLVISICKVNNIGLFSIFKSVFEVINNTDLNKNYLFIKKITEVYYICCILIFCLLGIYLTTFIWTANYIIAEYMDLKQIKLKMTSLAKSDIQSKMITRLIRE"
     primer_bind     complement(1613..1629)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1696..2284)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2455..3315)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3316..3420)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0325"
                     /note="membrane protein, putative"
                     /translation="MFSWDLYIINPLLIVIWLIVASYLFYKNSISKQKGLFYLEISSFWIVINFLIQIITNYIDSPILKSFSSSTLTILLFLSSYFLYATILNPFALWLTLKLQSRRIWIWISLFSCFLSVMIAFLSNVNITSIIFISLFLAVGISAQIIYFLFFNEQFNERLFPVFSSIKAGFVISFATFISYEVYSLLNLNLISNHNNYTNWIIFSLSLVCLIICLVVSIFVKERKIKVIKYKEDIVEQLQRYGYKVLIGLIVMSFLITSVNVIIKSDIFELFLVSKLKQQSYTSLNVWNYLQSFRLSFVLGQLLLGYLFYKLVIKVIGIVKSISILTSLTMFGVILITFIHNIYLLTIMMWVFGLFFFVMFYLWFGIALMWDYRSTKVSVLSTFLTVTFLTLSIWYLVISICKVNNIGLFSIFKSVFEVINNTDLNKNYLFIKKITEVYYICCILIFCLLGIYLTTFIWTANYIIAEYMDLKQIKLKMTSLAKSDIQSKMITRLIRE"
ORIGIN
        1 ATGTTTAGCT GGGATCTGTA TATTATTAAC CCGCTGCTGA TTGTGATTTG GCTGATTGTG
       61 GCGAGCTATC TGTTTTATAA AAACAGCATT AGCAAACAGA AAGGCCTGTT TTATCTGGAA
      121 ATTAGCAGCT TTTGGATTGT GATTAACTTT CTGATTCAGA TTATTACCAA CTATATTGAT
      181 AGCCCGATTC TGAAAAGCTT TAGCAGCAGC ACCCTGACCA TTCTGCTGTT TCTGAGCAGC
      241 TATTTTCTGT ATGCGACCAT TCTGAACCCG TTTGCGCTGT GGCTGACCCT GAAACTGCAG
      301 AGCCGCCGCA TTTGGATTTG GATTAGCCTG TTTAGCTGCT TTCTGAGCGT GATGATTGCG
      361 TTTCTGAGCA ACGTGAACAT TACCAGCATT ATTTTTATTA GCCTGTTTCT GGCGGTGGGC
      421 ATTAGCGCGC AGATTATTTA TTTTCTGTTT TTTAACGAAC AGTTTAACGA ACGCCTGTTT
      481 CCGGTGTTTA GCAGCATTAA AGCGGGCTTT GTGATTAGCT TTGCGACCTT TATTAGCTAT
      541 GAAGTGTATA GCCTGCTGAA CCTGAACCTG ATTAGCAACC ATAACAACTA TACCAACTGG
      601 ATTATTTTTA GCCTGAGCCT GGTGTGCCTG ATTATTTGCC TGGTGGTGAG CATTTTTGTG
      661 AAAGAACGCA AAATTAAAGT GATTAAATAT AAAGAAGATA TTGTGGAACA GCTGCAGCGC
      721 TATGGCTATA AAGTGCTGAT TGGCCTGATT GTGATGAGCT TTCTGATTAC CAGCGTGAAC
      781 GTGATTATTA AAAGCGATAT TTTTGAACTG TTTCTGGTGA GCAAACTGAA ACAGCAGAGC
      841 TATACCAGCC TGAACGTGTG GAACTATCTG CAGAGCTTTC GCCTGAGCTT TGTGCTGGGC
      901 CAGCTGCTGC TGGGCTATCT GTTTTATAAA CTGGTGATTA AAGTGATTGG CATTGTGAAA
      961 AGCATTAGCA TTCTGACCAG CCTGACCATG TTTGGCGTGA TTCTGATTAC CTTTATTCAT
     1021 AACATTTATC TGCTGACCAT TATGATGTGG GTGTTTGGCC TGTTCTTTTT TGTGATGTTT
     1081 TATCTGTGGT TTGGCATTGC GCTGATGTGG GATTATCGCA GCACCAAAGT GAGCGTGCTG
     1141 AGCACCTTTC TGACCGTGAC CTTTCTGACC CTGAGCATTT GGTATCTGGT GATTAGCATT
     1201 TGCAAAGTGA ACAACATTGG CCTGTTTAGC ATTTTTAAAA GCGTGTTTGA AGTGATTAAC
     1261 AACACCGATC TGAACAAAAA CTATCTGTTT ATTAAGAAAA TTACCGAAGT GTATTATATT
     1321 TGCTGCATTC TGATTTTTTG CCTGCTGGGC ATTTATCTGA CCACCTTTAT TTGGACCGCG
     1381 AACTATATTA TTGCGGAATA TATGGATCTG AAACAGATTA AACTGAAAAT GACCAGCCTG
     1441 GCGAAAAGCG ATATTCAGAG CAAAATGATT ACCCGCCTGA TTCGCGAGTG AAGAGCTTAG
     1501 AGACCGGTAC TAGTTCCTGC AGGGCTCGAG GTGTCAATCG TCGGAGCCGC TGAGCAATAA
     1561 CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCA TGGTCATAGC
     1621 TGTTTCCTGA GAGCTTGGCA GGTGATGACA CACATTAACA AATTTCGTGA GGAGTCTCCA
     1681 GAAGAATGCC ATTAATTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG
     1741 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG
     1801 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT
     1861 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT
     1921 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG
     1981 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT
     2041 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT
     2101 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT
     2161 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC
     2221 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC
     2281 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG
     2341 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA
     2401 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA
     2461 ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC
     2521 CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC
     2581 TGCAATGATA CCGCGAGAAC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC
     2641 AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT
     2701 TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT
     2761 TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC
     2821 CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG
     2881 CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT
     2941 TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC
     3001 TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG
     3061 CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT
     3121 TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC
     3181 GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC
     3241 TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA
     3301 ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG
     3361 TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG
     3421 CACCTGCACC AGTCAGTAAA ACGACGGCCA GTAGTCAAAA GCCTCCGACC GGAGGCTTTT
     3481 GACTTGGTCA GGTGGAGTGA ATTCGCGGCC GCTTCTAGAG GCTCTTCGTA ATTACGAAGA
     3541 GGTCTCTA
//