LOCUS       Exported     2894 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2894
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      907..954
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
                     /label="T7 terminator"
     primer_bind     2782..2798
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2799..2830
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0327"
                     /note="Putative ScpA segregation and condensation protein A"
                     /translation="MKHWQELTIDQFSGPIELLWLMIKEKKLDIIELSLIEIVDQYLAYIKQNQQLDIEIASEYLIIASQLIELKSRHLLFKDQQVDQEQVVDYDDLVYQISQYNQIKEISDRLFNAQEAYLQTFSKKRSKQNFKKDLVFENPDPLIDLNDLDLDKLTEIFYSVITNSNAFKYQADFDLETEIYQTLTTPSLTVHEVILDVVNKITSQKLKEWKLEELLEILELNLKNFVVIFLAVLDLVRYQILVIDSIDDQIYISLRKEVIENENLIAQQLEVIANESTI"
     primer_bind     complement(959..975)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1042..1630)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1801..2661)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2662..2766)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0327"
                     /note="Putative ScpA segregation and condensation protein A"
                     /translation="MKHWQELTIDQFSGPIELLWLMIKEKKLDIIELSLIEIVDQYLAYIKQNQQLDIEIASEYLIIASQLIELKSRHLLFKDQQVDQEQVVDYDDLVYQISQYNQIKEISDRLFNAQEAYLQTFSKKRSKQNFKKDLVFENPDPLIDLNDLDLDKLTEIFYSVITNSNAFKYQADFDLETEIYQTLTTPSLTVHEVILDVVNKITSQKLKEWKLEELLEILELNLKNFVVIFLAVLDLVRYQILVIDSIDDQIYISLRKEVIENENLIAQQLEVIANESTI"
ORIGIN
        1 ATGAAACATT GGCAGGAACT GACCATTGAT CAGTTTAGCG GCCCGATTGA ACTGCTGTGG
       61 CTGATGATTA AAGAGAAAAA ACTGGATATT ATTGAACTGA GCCTGATTGA AATTGTGGAT
      121 CAGTATCTGG CGTATATTAA ACAGAACCAG CAGCTGGATA TTGAAATTGC GAGCGAATAT
      181 CTGATTATTG CGAGCCAGCT GATTGAACTG AAAAGCCGCC ATCTGCTGTT TAAAGATCAA
      241 CAGGTGGATC AGGAACAGGT GGTGGATTAT GATGATCTGG TGTATCAGAT TAGCCAGTAT
      301 AACCAGATTA AAGAAATTAG CGATCGCCTG TTTAACGCGC AGGAAGCGTA TCTGCAGACC
      361 TTTAGCAAAA AACGCAGCAA ACAGAACTTT AAAAAAGATC TGGTGTTTGA AAACCCGGAT
      421 CCGCTGATTG ATCTGAACGA TCTGGATCTG GATAAACTGA CCGAAATTTT TTATAGCGTG
      481 ATTACCAACA GCAACGCGTT TAAATATCAG GCGGATTTTG ATCTGGAAAC CGAAATTTAT
      541 CAGACCCTGA CCACCCCGAG CCTGACCGTG CATGAAGTGA TTCTGGATGT GGTGAACAAA
      601 ATTACCAGCC AGAAACTGAA AGAATGGAAA CTGGAAGAAC TGCTGGAAAT TCTGGAACTG
      661 AACCTGAAAA ACTTTGTGGT GATTTTTCTG GCGGTGCTGG ATCTGGTGCG CTATCAGATT
      721 CTGGTGATTG ATAGCATTGA TGATCAGATT TATATTAGCC TGCGCAAAGA AGTGATTGAA
      781 AACGAAAACC TGATTGCGCA GCAGCTGGAA GTGATTGCGA ACGAAAGCAC CATTTGAAGA
      841 GCTTAGAGAC CGGTACTAGT TCCTGCAGGG CTCGAGGTGT CAATCGTCGG AGCCGCTGAG
      901 CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCATGGT
      961 CATAGCTGTT TCCTGAGAGC TTGGCAGGTG ATGACACACA TTAACAAATT TCGTGAGGAG
     1021 TCTCCAGAAG AATGCCATTA ATTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA
     1081 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC
     1141 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC
     1201 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG
     1261 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA
     1321 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC
     1381 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC
     1441 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG
     1501 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA
     1561 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA
     1621 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA
     1681 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT
     1741 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG
     1801 TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT
     1861 AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC
     1921 CAGTGCTGCA ATGATACCGC GAGAACCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA
     1981 CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA
     2041 GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA
     2101 CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT
     2161 CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC
     2221 GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT
     2281 CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC
     2341 TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG
     2401 CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT
     2461 CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC
     2521 CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG
     2581 CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC
     2641 ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG
     2701 TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT
     2761 TCCGCGCACC TGCACCAGTC AGTAAAACGA CGGCCAGTAG TCAAAAGCCT CCGACCGGAG
     2821 GCTTTTGACT TGGTCAGGTG GAGTGAATTC GCGGCCGCTT CTAGAGGCTC TTCGTAATTA
     2881 CGAAGAGGTC TCTA
//