LOCUS       Exported     2687 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2687
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      700..747
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2575..2591
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2592..2623
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0328"
                     /note="Putative ScpB segregation and condensation protein B"
                     /translation="MNQQYSAIIEGLLFIYGDDGVSLLDIQTVLDNLKPTEIKEIIIELNKKYLADPSSAFCIQTYKKNNYRLQTKPELHEYFAKLEQYNENKKLSHSTIEVLSIIAYKQPITKQQIDEIRNVDSTYQLYKLREKKLIKVVGKDLENNRSNLYGITDNFFKVFNIKGGIEELPTISDDDIKQAIENNNEIKQEQQNLDLYGNIDDFNLDSQNQ"
     primer_bind     complement(752..768)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(835..1423)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1594..2454)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(2455..2559)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0328"
                     /note="Putative ScpB segregation and condensation protein B"
                     /translation="MNQQYSAIIEGLLFIYGDDGVSLLDIQTVLDNLKPTEIKEIIIELNKKYLADPSSAFCIQTYKKNNYRLQTKPELHEYFAKLEQYNENKKLSHSTIEVLSIIAYKQPITKQQIDEIRNVDSTYQLYKLREKKLIKVVGKDLENNRSNLYGITDNFFKVFNIKGGIEELPTISDDDIKQAIENNNEIKQEQQNLDLYGNIDDFNLDSQNQ"
ORIGIN
        1 ATGAACCAAC AGTACTCAGC AATTATCGAA GGATTGTTGT TTATTTATGG TGACGACGGT
       61 GTGAGCCTTT TGGATATTCA AACAGTTCTG GACAATTTGA AACCGACTGA GATCAAGGAG
      121 ATTATCATCG AACTTAACAA AAAATACTTG GCTGACCCCT CCTCGGCATT TTGTATTCAA
      181 ACTTACAAGA AGAATAATTA TCGCCTTCAA ACCAAGCCCG AGCTTCATGA ATACTTCGCG
      241 AAGTTGGAAC AATATAACGA GAATAAGAAA CTGTCCCACT CAACCATCGA AGTGTTATCC
      301 ATCATTGCCT ACAAACAGCC AATCACCAAA CAGCAAATTG ATGAGATTCG TAATGTTGAC
      361 TCGACGTATC AGCTTTATAA GTTGCGTGAA AAGAAATTGA TCAAAGTCGT GGGCAAAGAT
      421 TTAGAGAATA ATCGCTCAAA TTTGTACGGC ATTACTGACA ATTTCTTTAA GGTATTTAAC
      481 ATTAAAGGCG GGATTGAGGA ACTTCCCACC ATCAGTGATG ATGATATTAA GCAGGCCATT
      541 GAGAACAATA ATGAAATTAA GCAAGAGCAA CAAAACCTGG ATCTTTATGG GAATATCGAT
      601 GATTTCAATT TAGATTCACA GAATCAGTGA AGAGCTTAGA GACCGGTACT AGTTCCTGCA
      661 GGGCTCGAGG TGTCAATCGT CGGAGCCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC
      721 TCTAAACGGG TCTTGAGGGG TTTTTTGCAT GGTCATAGCT GTTTCCTGAG AGCTTGGCAG
      781 GTGATGACAC ACATTAACAA ATTTCGTGAG GAGTCTCCAG AAGAATGCCA TTAATTTCCA
      841 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA
      901 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC
      961 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC
     1021 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT
     1081 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG
     1141 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG
     1201 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA
     1261 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG
     1321 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT
     1381 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT
     1441 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG
     1501 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT
     1561 CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC
     1621 TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT
     1681 AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGAACC
     1741 ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG
     1801 AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG
     1861 AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT
     1921 GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG
     1981 AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT
     2041 TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC
     2101 TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC
     2161 ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA
     2221 TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG
     2281 AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC
     2341 CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG
     2401 GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT
     2461 CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT
     2521 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACCTGCACCA GTCAGTAAAA
     2581 CGACGGCCAG TAGTCAAAAG CCTCCGACCG GAGGCTTTTG ACTTGGTCAG GTGGAGTGAA
     2641 TTCGCGGCCG CTTCTAGAGG CTCTTCGTAA TTACGAAGAG GTCTCTA
//