LOCUS Exported 3587 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3587 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 1600..1647 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 3475..3491 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 3492..3523 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 1..0 /label="MMSYN1_0359" /note="RNase_Y: ribonuclease Y" /translation="MEENIIIILSVFLGIFFICFVISSVILLYFWKSKSRKHLIEQYTKEAKQAKKQVLANGYKEISEAKMLFLKRSELEKNELDRVKEQLDLRANDLKRSQEIVESKSQRLDAGILDLEKRKFLVDQKEEYLIKLLEDVSGLTKYQAKELLIKQIKNKSEKELISILKNAELQAHSKAKIISNNILISAMERIKVELTSQRTTNIVKLPSDDLKGRIIGKDGRNMKTFEQIGGVDIVVDETPNVVVVSSFNPIRREIATRTLEQLIIDGRIQPVKIENELKKQEQELEYIIQETGLNTIKELNINDIDIELVKLIGKLKFRTSYGQNVLAHSIEVAKLSGAIASELGLDVEKAIRAGLLHDIGKAIDFEKQGSHVVLGAEIARKYNEDPIIINSIESHHEDKEKSSEIAAIVAIADSISASRPGARYNAIDEFILRMHEIEKIGNSIPGVAKTYALQSGRQIRLIVDPLVASDLDLALILEKMKEQIKDKVIIPGEITITVIREKKETDILK" primer_bind complement(1652..1668) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1735..2323) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2494..3354) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(3355..3459) /label="bla promoter" /note="ampicillin resistance gene promoter" CDS complement(1..0) /label="MMSYN1_0359" /note="RNase_Y: ribonuclease Y" /translation="MEENIIIILSVFLGIFFICFVISSVILLYFWKSKSRKHLIEQYTKEAKQAKKQVLANGYKEISEAKMLFLKRSELEKNELDRVKEQLDLRANDLKRSQEIVESKSQRLDAGILDLEKRKFLVDQKEEYLIKLLEDVSGLTKYQAKELLIKQIKNKSEKELISILKNAELQAHSKAKIISNNILISAMERIKVELTSQRTTNIVKLPSDDLKGRIIGKDGRNMKTFEQIGGVDIVVDETPNVVVVSSFNPIRREIATRTLEQLIIDGRIQPVKIENELKKQEQELEYIIQETGLNTIKELNINDIDIELVKLIGKLKFRTSYGQNVLAHSIEVAKLSGAIASELGLDVEKAIRAGLLHDIGKAIDFEKQGSHVVLGAEIARKYNEDPIIINSIESHHEDKEKSSEIAAIVAIADSISASRPGARYNAIDEFILRMHEIEKIGNSIPGVAKTYALQSGRQIRLIVDPLVASDLDLALILEKMKEQIKDKVIIPGEITITVIREKKETDILK" ORIGIN 1 ATGGAGGAGA ATATCATTAT CATTCTTTCA GTTTTTCTGG GCATATTTTT CATTTGTTTT 61 GTCATTTCTA GTGTCATCCT TCTGTACTTC TGGAAGTCCA AGAGTCGCAA ACATCTTATC 121 GAACAGTATA CCAAAGAGGC CAAACAAGCC AAGAAACAGG TGTTGGCTAA CGGTTATAAA 181 GAGATTTCAG AGGCCAAGAT GTTATTCCTT AAGCGCTCAG AGCTTGAAAA GAATGAACTT 241 GATCGCGTGA AAGAACAATT GGACTTACGT GCCAACGATC TTAAACGTTC TCAAGAGATC 301 GTGGAATCTA AGAGTCAGCG TTTAGATGCC GGCATTCTGG ATTTGGAAAA ACGCAAGTTT 361 CTGGTCGACC AGAAGGAGGA ATATCTTATT AAGCTGCTTG AGGACGTTTC TGGCCTTACA 421 AAATATCAGG CGAAAGAGCT TCTTATTAAA CAGATCAAGA ACAAGTCAGA AAAGGAACTT 481 ATCTCGATCC TGAAGAATGC AGAGTTACAG GCCCATTCGA AAGCGAAGAT TATCAGTAAT 541 AATATCCTGA TCTCCGCTAT GGAGCGTATT AAAGTAGAGC TTACATCCCA ACGCACGACT 601 AACATTGTTA AATTGCCTTC AGACGATCTT AAAGGTCGCA TCATTGGTAA AGACGGCCGT 661 AACATGAAAA CATTCGAACA GATTGGCGGT GTGGACATTG TGGTTGATGA AACACCGAAT 721 GTCGTAGTTG TGAGCTCCTT CAACCCCATT CGTCGTGAAA TCGCGACCCG TACGCTGGAA 781 CAGCTTATTA TCGACGGTCG TATTCAGCCC GTTAAAATCG AGAACGAGTT GAAAAAGCAG 841 GAGCAGGAGC TTGAGTATAT CATCCAAGAA ACTGGATTGA ATACTATCAA AGAGTTGAAT 901 ATTAATGATA TCGATATTGA ATTGGTCAAG TTGATTGGAA AACTTAAGTT TCGTACAAGC 961 TACGGCCAGA ATGTGTTGGC ACACAGCATT GAAGTGGCAA AGCTTTCTGG CGCAATCGCA 1021 TCGGAACTGG GTCTTGATGT AGAAAAAGCA ATTCGTGCAG GATTACTGCA CGATATCGGT 1081 AAAGCGATCG ATTTCGAAAA GCAAGGTAGC CACGTCGTAT TAGGAGCCGA GATCGCGCGC 1141 AAGTATAATG AAGATCCGAT TATTATCAAT AGCATCGAGT CGCATCATGA GGATAAGGAA 1201 AAATCGAGCG AGATCGCAGC TATTGTAGCT ATTGCTGATA GTATCTCGGC CAGCCGTCCT 1261 GGCGCACGCT ACAATGCTAT CGACGAATTC ATCTTGCGTA TGCACGAAAT TGAGAAGATC 1321 GGCAATTCGA TCCCTGGCGT AGCGAAAACA TACGCCTTAC AGTCGGGGCG CCAGATTCGT 1381 TTGATTGTGG ATCCGCTTGT AGCGTCCGAT CTGGATTTAG CGCTGATTTT AGAAAAAATG 1441 AAGGAGCAGA TTAAGGATAA AGTCATCATC CCGGGAGAGA TCACCATCAC AGTAATCCGT 1501 GAAAAGAAAG AAACAGATAT CCTGAAGTGA AGAGCTTAGA GACCGGTACT AGTTCCTGCA 1561 GGGCTCGAGG TGTCAATCGT CGGAGCCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC 1621 TCTAAACGGG TCTTGAGGGG TTTTTTGCAT GGTCATAGCT GTTTCCTGAG AGCTTGGCAG 1681 GTGATGACAC ACATTAACAA ATTTCGTGAG GAGTCTCCAG AAGAATGCCA TTAATTTCCA 1741 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 1801 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 1861 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 1921 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 1981 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 2041 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 2101 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 2161 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 2221 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 2281 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 2341 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 2401 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 2461 CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC 2521 TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT 2581 AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGAACC 2641 ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG 2701 AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG 2761 AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT 2821 GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG 2881 AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT 2941 TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC 3001 TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC 3061 ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA 3121 TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG 3181 AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC 3241 CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG 3301 GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT 3361 CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT 3421 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACCTGCACCA GTCAGTAAAA 3481 CGACGGCCAG TAGTCAAAAG CCTCCGACCG GAGGCTTTTG ACTTGGTCAG GTGGAGTGAA 3541 TTCGCGGCCG CTTCTAGAGG CTCTTCGTAA TTACGAAGAG GTCTCTA //