LOCUS       Exported     3401 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3401
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      1414..1461
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3289..3305
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3306..3337
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /translation="MGFGDFLSKRMQKSIEKNMKNSTLNEENIKETLKEIRLSLLEADVNIEAAKEIINNVKQKALGGYISEGASAHQQMIKIVHEELVNILGKENAPLDINKKPSVVMMVGLQGSGKTTTANKLAYLLNKKNKKKVLLVGLDIYRPGAIEQLVQLGQKTNTQVFEKGKQDPVKTAEQALQYAKENNFDVVILDTAGRLQVDQFLMKELDNLKKKTSPNEILLVVDGMSGQEIINVTNEFNSKLKLSGVVVTKLDGDARGGATLSISYLTKLPIKFIGEGEGYNALAAFYPKRMADRLMGMGDIETLFERAVENIDERSIQKTMNRMFLGQFDLEDLRNQLAQIAKMGSLNKLMKMLPINKVSESQIQDAQRKLAVFSILMDSMTLKERRDPRVLKAISRKNRIIKGSGRSEKEFNELINSFEKGKKQVLEITKMIKSGRMPNLSKGGFKF"
                     /label="MMSYN1_0360"
                     /note="ffh: signal recognition particle protein"
     primer_bind     complement(1466..1482)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(1549..2137)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2308..3168)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(3169..3273)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0360"
                     /note="ffh: signal recognition particle protein"
                     /translation="MGFGDFLSKRMQKSIEKNMKNSTLNEENIKETLKEIRLSLLEADVNIEAAKEIINNVKQKALGGYISEGASAHQQMIKIVHEELVNILGKENAPLDINKKPSVVMMVGLQGSGKTTTANKLAYLLNKKNKKKVLLVGLDIYRPGAIEQLVQLGQKTNTQVFEKGKQDPVKTAEQALQYAKENNFDVVILDTAGRLQVDQFLMKELDNLKKKTSPNEILLVVDGMSGQEIINVTNEFNSKLKLSGVVVTKLDGDARGGATLSISYLTKLPIKFIGEGEGYNALAAFYPKRMADRLMGMGDIETLFERAVENIDERSIQKTMNRMFLGQFDLEDLRNQLAQIAKMGSLNKLMKMLPINKVSESQIQDAQRKLAVFSILMDSMTLKERRDPRVLKAISRKNRIIKGSGRSEKEFNELINSFEKGKKQVLEITKMIKSGRMPNLSKGGFKF"
ORIGIN
        1 ATGGGATTTG GCGATTTTCT TTCGAAACGC ATGCAAAAGT CTATTGAAAA GAACATGAAA
       61 AACTCAACTT TAAACGAGGA AAACATTAAA GAAACCTTGA AAGAAATTCG TTTGTCACTT
      121 TTAGAAGCTG ACGTTAACAT TGAAGCAGCA AAAGAAATCA TCAACAATGT AAAACAAAAA
      181 GCGTTGGGCG GGTATATCAG CGAGGGAGCG TCGGCCCACC AGCAGATGAT CAAAATTGTG
      241 CACGAGGAGT TAGTGAACAT CCTGGGCAAG GAAAATGCAC CCCTTGACAT CAACAAAAAA
      301 CCCTCAGTCG TCATGATGGT CGGCTTGCAA GGCTCTGGCA AGACTACCAC AGCAAATAAA
      361 TTAGCGTATC TGTTGAATAA GAAGAATAAG AAAAAGGTGC TTTTAGTTGG TCTTGATATT
      421 TACCGCCCTG GTGCAATCGA GCAATTAGTC CAATTAGGTC AAAAGACTAA CACTCAGGTC
      481 TTTGAGAAGG GTAAACAGGA CCCTGTTAAA ACAGCGGAGC AGGCCCTTCA ATACGCAAAA
      541 GAAAATAACT TTGATGTGGT TATCCTTGAC ACCGCTGGAC GCCTTCAAGT AGACCAATTT
      601 CTTATGAAGG AGTTGGACAA TCTTAAGAAA AAGACCAGCC CGAATGAGAT TTTGCTGGTT
      661 GTGGACGGTA TGAGCGGGCA GGAAATCATT AACGTAACCA ACGAATTCAA CAGTAAATTG
      721 AAATTATCGG GGGTCGTGGT AACTAAGCTG GACGGCGACG CCCGTGGTGG GGCAACATTG
      781 TCGATCTCGT ATCTTACAAA ATTGCCCATC AAGTTCATTG GTGAAGGGGA GGGATATAAT
      841 GCGCTTGCCG CCTTCTACCC GAAGCGTATG GCTGATCGTT TGATGGGTAT GGGGGACATC
      901 GAAACGCTTT TCGAGCGTGC TGTTGAAAAC ATTGATGAAC GTAGTATCCA AAAGACGATG
      961 AATCGCATGT TTCTGGGCCA GTTCGATCTT GAGGACTTGC GTAATCAACT TGCACAAATT
     1021 GCGAAGATGG GTAGCTTAAA CAAGCTGATG AAAATGTTAC CGATCAATAA AGTTAGTGAA
     1081 AGTCAGATTC AGGATGCCCA ACGTAAATTG GCAGTATTTT CGATCCTTAT GGACTCGATG
     1141 ACATTAAAAG AACGTCGCGA TCCTCGTGTG TTGAAGGCTA TTTCTCGTAA GAATCGTATC
     1201 ATCAAGGGGT CAGGTCGCTC GGAAAAAGAG TTCAATGAGC TTATCAACTC CTTTGAGAAA
     1261 GGCAAAAAGC AAGTGCTTGA GATCACTAAA ATGATTAAGA GTGGTCGCAT GCCGAATTTA
     1321 AGCAAAGGCG GCTTCAAGTT TTGAAGAGCT TAGAGACCGG TACTAGTTCC TGCAGGGCTC
     1381 GAGGTGTCAA TCGTCGGAGC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA
     1441 CGGGTCTTGA GGGGTTTTTT GCATGGTCAT AGCTGTTTCC TGAGAGCTTG GCAGGTGATG
     1501 ACACACATTA ACAAATTTCG TGAGGAGTCT CCAGAAGAAT GCCATTAATT TCCATAGGCT
     1561 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC
     1621 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC
     1681 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC
     1741 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG
     1801 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA
     1861 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG
     1921 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA
     1981 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG
     2041 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG
     2101 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC
     2161 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC
     2221 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG
     2281 TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC
     2341 AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC
     2401 GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG AACCACGCTC
     2461 ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG
     2521 TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG
     2581 TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC
     2641 ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC
     2701 ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG
     2761 AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC
     2821 TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG
     2881 AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC
     2941 GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT
     3001 CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG
     3061 ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA
     3121 TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT
     3181 TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG
     3241 TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACCTGC ACCAGTCAGT AAAACGACGG
     3301 CCAGTAGTCA AAAGCCTCCG ACCGGAGGCT TTTGACTTGG TCAGGTGGAG TGAATTCGCG
     3361 GCCGCTTCTA GAGGCTCTTC GTAATTACGA AGAGGTCTCT A
//